Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-26T22:49:18.461Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of g.9476869G>A myeloperoxidase (MPO) gene polymorphism on the antioxidant activity of milk from Polish Holstein-Friesian cows of the Black-and-White variety (HO)

Published online by Cambridge University Press:  19 May 2017

Joanna Pokorska ,
Karolina Poskart ,
Dominika Kułaj ,
Andrzej Ochrem ,
Magdalena Dusza ,
Zygmunt Gil ,
Ewa Świętek  and
Joanna Makulska
Joanna Pokorska*
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Karolina Poskart
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Dominika Kułaj
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Andrzej Ochrem
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Magdalena Dusza
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Zygmunt Gil
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Ewa Świętek
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Joanna Makulska
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Myeloperoxidase (MPO) is an important enzyme, which is one of the components of the antibacterial system in neutrophils and monocytes. MPO participates in the inflammatory response in multiple locations in the body, including the mammary glands. As a result of the activity of MPO, many oxidising compounds as well as reactive oxygen species are generated. It seems that myeloperoxidase may be a marker linking inflammation processes and oxidative stress. So far, there are no literature data on the association between the MPO gene polymorphism and the antioxidant properties of milk. The aim of the study was to analyse the effect of g.9476869G > A polymorphism of myeloperoxidase (MPO) gene and age of cows on the antioxidant activity of milk and other milk traits in Polish Holstein-Friesian cows. Polymorphism of MPO gene was identified by the PCR-RFLP method using the HphI endonuclease. The total antioxidant capacity of milk samples was measured by the Trolox Equivalent Antioxidant Capacity (TEAC) method. It was found that the GG genotype was the most frequent (0·606). The genotype at the tested MPO locus and the age of the animals affected the antioxidant activity of milk. Milk from cows with the GA genotype was characterised by a significantly higher antioxidant activity than milk from cows with the GG genotype (P < 0·0001). The analysis of interaction showed that cows with the GA genotype and older than 6·5 years produced milk with a significantly higher antioxidant activity compared with younger cows with the same genotype (P < 0·0001), as well as cows with the GG genotype of all ages (P < 0·0001).

Keywords

Myeloperoxidasemilk antioxidant activitygene polymorphism
Type
Research Article
Information
Journal of Dairy Research , Volume 84 , Issue 2 , May 2017 , pp. 159 - 164
Copyright
Copyright © Proprietors of Journal of Dairy Research 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Castillo, C, Hernández, J, Valverde, I, Pereira, V, Sotillo, J, Alonso, ML & Benedito, JL 2006 Plasma malonaldehyde (MDA) and total antioxidant status (TAS) during lactation in dairy cows. Research in Veterinary Science 80 133–139 Google Scholar
Celi, P 2010 The role of oxidative stress in small ruminats’ health and production. Revista Brasileira the Zootecnia 39 348–363 Google Scholar
Čipak Gašparović, A, Lovaković, T & Žarković, N 2010 Oxidative stress and antioxidants: biological response modifiers of oxidative homeostasis in cancer. Periodicum Biologorum 112 433–439 Google Scholar
Cooper, DN 2010 Functional intronic polymorphisms: buried treasure awaiting discovery within our genes. Human Genomic 4 284 CrossRefGoogle ScholarPubMed
Decker, EA, Livisay, SA & Zhou, S 2000 Mechanism of endogenous skeletal muscle antioxidants: chemical and physical aspects. In Antioxidants in Muscle Foods: Nutritional Strategies to Improve Quality, pp. 25–60 (Eds Decker, EA, Faustman, C & Lopez-Bote, CJ). John Wiley & Sons, Inc. Publication; NY, USA Google Scholar
Friguet, B 2006 Oxidized protein degradation and repair in ageing and oxidative stress. FEBS Letters 580 2910–2916 Google Scholar
Gurmessa, J, & Melaku, A 2012 Effect of lactation stage, pregnancy, parity and age on yield and major components of raw milk in bred cross Holstein Fresian cows. World Journal of Dairy & Food Sciences 7 146–149 Google Scholar
Hawkins, CL, Pattison, DI & Davies, MJ 2003 Hypochlorite-induced oxidation of amino acids, peptides and proteins. Amino Acids 25 259–274 Google Scholar
Klebanoff, SJ 2005 Myeloperoxidase: friend and foe. Journal of Leukocyte Biology 77 598–625 Google Scholar
Król, K & Konopka, T 2003 Reactive oxygen species and antioxidant mechanisms in the pathogenesis of periodontitis. Dental and Medical Problems 40 121–128 Google Scholar
Kuczyńska, B, Nałęcz-Tarwacka, T & Puppel, K 2013 Bioactive components as an indicator of the health-beneficial quality of milk. Medycyna Rodzinna 1 11–18 Google Scholar
Locksley, RM, Wilson, ChB & Klebanoff, SJ 1983 Increased respiratory burst in myeloperoxidase – deficient monocytes. Blood 62 902–909 Google Scholar
Lohrke, B, Viergutz, T, Kanitz, W, Gollnitz, K, Becker, F, Hurtienne, A & Schweigert, FJ 2004 High milk yield in dairy cows associated with oxidant stress. Online Journal of Veterinary Research 8 70–78 Google Scholar
Marchetti, C, Patriarca, P, Solero, GP, Baralle, FE & Romano, M 2004 Genetic studies on myeloperoxidase deficiency in Italy. Japanese Journal of Infectious Diseases 57 10–12 Google ScholarPubMed
Michalak, A, Krzeszowiak, J & Markiewicz-Górka, I 2014 Starzenie się organizmu a stres oksydacyjny oraz zmniejszona sprawność systemów naprawczych. Postępy Higieny i Medycyny Doświadczalnej 68 1483–1491 Google Scholar
Patrushev, LI & Kovalenko, TF 2014 Functions of noncoding sequences in mammalian genomes. Biochemistry (Moscow) 79 1442–1469 Google Scholar
Pilarska, M 2014 Wpływ pory roku i kolejnej laktacji na wydajność krów i parametry fizykochemiczne mleka. Wiadomości Zootechniczne 2 3–12 Google Scholar
Pisulewski, P 2007. Potencjał przeciwutleniający żywności pochodzenia zwierzęcego. In Przeciwutleniacze w żywności. Aspekty zdrowotne, technologiczne, molekularne i analityczne, pp. 217–229. red. (Ed. Grajek, W).Warszawa: Wydawnictwo Naukowo Techniczne.Google Scholar
Plust, D, Czerniejewska-Surma, B, Domiszewski, Z & Bienkiewicz, G 2011 Zawartość polifenoli, właściwości przeciwutleniające oraz zdolności redukujące naparów herbat białych liściastych. Folia Pomeranae Universitatis Technologiae Stetinensis, seria Agricultura. Alimentaria, Piscaria et Zootechnica 286 47–52 Google Scholar
Pokorska, J, Kułaj, D, Dusza, M, Żychlińska-Buczek, J & Makulska, J 2016 New Rapid Method of DNA Isolation from Milk Somatic Cells. Animal Biotechnology 27 113–117 Google Scholar
Rahal, A, Kumar, A, Singh, V, Yadav, B, Tiwari, R, Chakraborty, S & Dhama, K 2014 Oxidative stress, prooxidants, and antioxidants: the interplay. BioMed Research International 2014 761264 Google Scholar
Re, R, Pellegrini, N, Proteggente, A, Pannala, A, Yang, M & Rice-Evans, C 1999 Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine 26 1231–1237 Google Scholar
Roberts, CK & Sindhu, KK 2009 Oxidative stress and metabolic syndrome. Life Sciences 84 705–712 Google Scholar
Untergasser, A, Nijveen, H, Rao, X, Bisseling, T, Geurts, R & Leunissen, JAM 2007 Primer3Plus. an enhanced web interface to Primer3. Nuceic Acids Research 35 W71–W74 Google Scholar
Van den Berg, R, Haenen, GRMM, Van den Berg, H & Bast, A 1999 Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. Food Chemistry 66 511–517 Google Scholar