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Effect of different heat treatments on the strong binding interactions between whey proteins and milk fat globules in whole milk

Published online by Cambridge University Press:  01 June 2009

Milena Corredig  and
Douglas G. Dalgleish
Milena Corredig
Affiliation:
Department of Food Science, University of Guelph, Guelph, Ontario, Canada NIG 2W1
Douglas G. Dalgleish
Affiliation:
Department of Food Science, University of Guelph, Guelph, Ontario, Canada NIG 2W1
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Summary

The heat-induced binding of whey proteins to milk fat globule membranes in whole milk was investigated by quantitative electrophoresis and laser scanning densitometry. Both α-lactalbumin and β-lactoglobulin bound to the surfaces of fat globules when milk was heated in a water bath in the temperature range 65–85 °C. The interaction behaviour of α-lactalbumin did not seem to change with temperature, and the total amount of protein bound was ∼ 0·2 mg/g fat contained in the cream. The quantity of βlactoglobulin interacting with the milk fat globules increased with temperature from 02 to 0·7 mg/g fat between 65° and 85 °C. Even in whole milk heated at batch pasteurization temperatures (60–65 °C), α-lactalbumin and β-lactoglobulin were found attached to the fat globules. The interactions of the whey proteins with intact fat globule membranes were also investigated in milk heated in an industrial system (a pilot scale UHT and high temperature short time module), and the results were compared with those from the laboratory treatment (simple batch heating). The binding of the whey proteins to fat globules differed between milk heated by UHT using indirect steam heating or direct steam injection (DSI). However, the surface load in milk treated by DSI was not comparable to that of milk treated by batch heating or indirect steam heating, because of the changes in fat globule size and membrane composition caused by the DSI process.

Type
Original Articles
Information
Journal of Dairy Research , Volume 63 , Issue 3 , August 1996 , pp. 441 - 449
Copyright
Copyright © Proprietors of Journal of Dairy Research 1996

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References

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