Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-27T02:02:17.810Z Has data issue: false hasContentIssue false
  • English
  • Français

Discrete phosphorylation generates the electrophoretic heterogeneity of ovine β-casein

Published online by Cambridge University Press:  01 June 2009

Lina Chianese ,
Giuseppina Garro ,
Pasquale Ferranti ,
Antonio Malorni ,
Francesco Addeo ,
Araceli Rabasco  and
Pilar Molina Pons
Lina Chianese
Affiliation:
Dipartimento di Scienza degli Alimenti, Facoltà di Agraria, Università degli Studi di Napoli Federico II, Parco Gussone, 1-80055 Portici, Italia
Giuseppina Garro
Affiliation:
Dipartimento di Scienza degli Alimenti, Facoltà di Agraria, Università degli Studi di Napoli Federico II, Parco Gussone, 1-80055 Portici, Italia
Pasquale Ferranti
Affiliation:
Dipartimento di Scienza degli Alimenti, Facoltà di Agraria, Università degli Studi di Napoli Federico II, Parco Gussone, 1-80055 Portici, Italia
Antonio Malorni
Affiliation:
Servizio di Spettrometria di Massa, CNR, Via Pansini 5, 1-80131 Napoli, Istituto per la Chimica delle Molecole di Interesse Biologic, CNR, 1-80072 Arcofeli, Italia
Francesco Addeo
Affiliation:
Dipartimento di Scienza degli Alimenti, Facoltà di Agraria, Università degli Studi di Napoli Federico II, Parco Gussone, 1-80055 Portici, Italia
Araceli Rabasco
Affiliation:
Departamento Genetica, Facultad de Veterinar, Universidad de Estremadu, E-10071 Caceres, España
Pilar Molina Pons
Affiliation:
Departamento de Ciencia Animal, Universidad Politecnica de Valencia, E-46071 Valencia, España
Get access Rights & Permissions [Opens in a new window]

Summary

The heterogeneity of ovine β-casein, apparent after discontinuous gel electrophoresis at alkaline pH and gel isoelectric focusing either by staining with Coomassie blue or immunoblotting, was investigated in detail. Electrospray mass spectrometry was used to characterize ovine casein by direct examination of whole casein samples. β-Casein in individual milks consisted of several components which were characterized through the accurate determination of their molecular masses. It was demonstrated that protein species occurring simultaneously in individual ovine milks differed mainly in the number of phosphate groups.

Type
Original articles
Information
Journal of Dairy Research , Volume 62 , Issue 1 , February 1995 , pp. 89 - 100
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Arave, C. W., Gillett, T. A., Price, D. A. & Matthews, D. H. 1973 Polymorphisms in caseins of sheep milk. Journal of Animal Science 36 241–244CrossRefGoogle ScholarPubMed
Asohaffenbukg, R. & Drewry, J. 1959 New procedure for the routine determination of the various non-casein proteins of milk. 15th International Dairy Congress, London 3 1631–1637Google Scholar
Blakesley, R. W. & Boezi, J. A. 1977 A new staining technique for proteins in polyacrylamide gels using Coomassie brilliant blue G 250. Analytical Biochemistry 82 580–582CrossRefGoogle Scholar
Chianese, L., Addeo, F., Rabasco, A. & Molina Pons, P. 1991 [Discovery of a novel β-casein variant in ovine milk.] Proceedings of the XL Vth S.I.S. Vet. (Italian Veterinary Science Society National Congress, Palermo 45 222 (Abstr.))Google Scholar
Chianese, L., Garro, G., Nicolai, M. A., Mauriello, R., Ferranti, P., Pizzano, R., Cappuccio, U., Laezza, P., Addeo, F., Ramunno, L., Rando, A. & Rubino, R. 1993 The nature of β-casein heterogeneity in caprine milk. Lait 73 533–547CrossRefGoogle Scholar
Chianese, L., Mauriello, R., Moio, L., Intorcia, N. & Addeo, F. 1992 Determination of ovine casein heterogeneity using gel electrophoresis and immunochemical techniques. Journal of Dairy Research 59 39–47CrossRefGoogle ScholarPubMed
Chiofalo, L. & Micari, P. 1987 [Present knowledge of protein variants in ovine populations bred in Sicily. Experimental observations.] Scienza e Tecnica Lattiero-Casearia 38 104–114Google Scholar
European Economic Community 1992 [A reference method to detect bovine casein in ovine cheese.] (EEC Regulations no. 690/92.) Official Journal of the Italian Republic no. 44Google Scholar
Green, B. N., Oliver, R. W. A., Falick, A. M., Shackleton, C. H. L., Roitman, E. & Witowska, H. E. 1990 Electrospray MS, LS/MS and MS/MS for the rapid detection and characterization of variant hemoglobins. In Biological Mass Spectrometry, pp. 129–146 (Eds Burlingame, A. L. and McCloskey, J. A.). Amsterdam: ElsevierGoogle Scholar
Greenberg, R. 1986 The relationship of maternal factors to composition and structure of caseins. In Human Lactation 2 Maternal and Environmental Factors, pp. 187–193 (Eds Hamosh, M. and Goldman, A. S.). New York: Plenum PressCrossRefGoogle Scholar
Greenberg, R. & Groves, M. L. 1979 Human β-casein. Journal of Dairy Research 46 235–239CrossRefGoogle ScholarPubMed
King, J. W. B. 1967 The caseins of sheep's milk. In Polymorphismes Biochimiques des Animaux, pp. 427–431. Paris: INRA (European Conference on Animal Blood Groups and Biochemical Polymorphisms 10 1966)Google Scholar
Mercier, J. C. 1981 Phosphorylation of caseins. Present evidence for an amino acid triplet code posttranslationally recognized by specific kinases. Biochimie 63 1–17CrossRefGoogle ScholarPubMed
Mercier, J. C., Maubois, J. L., Poznanski, S. & Ribadeau-Dumas, B. 1968 [Preparative fractionation of bovine and ovine casein by DEAE-cellulose chromatography in a medium containing urea and 2- mercaptoethanol.] Bulletin de la Société de Chimie Biologique 50 521–530Google Scholar
Richardson, B. C. & Creamer, L. K. 1976 Comparative micelle structure. 5. The isolation and characterization of the major ovine caseins. New Zealand Journal of Dairy Science and Technology 11 46–53Google Scholar
Richardson, B. C. & Mercier, J. -C. 1979 The primary structure of ovine β-caseins. European Journal of Biochemistry 99 285–297Google ScholarPubMed
Shackleton, C. H. L., Falick, A. M., Green, B. N. & Witowska, H. E. 1991 Electrospray mass spectrometry in the clinical diagnosis of variant hemoglobins. Journal of Chromatography: Biomedical Applications 562 175–190CrossRefGoogle ScholarPubMed
Trieu-Cuot, P. & Gripon, J. -C. 1981 Electrofocusing and two-dimensional electrophoresis of bovine caseins. Journal of Dairy Research 48 303–310CrossRefGoogle Scholar