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Determination of gradient elution conditions for the separation of peptide mixtures by reversed-phase high-performance liquid chromatography: bovine β-casein tryptic digest

Published online by Cambridge University Press:  01 June 2009

Christophe Carles  and
Bruno Ribadeau-Dumas
Christophe Carles
Affiliation:
Institut National de la Recherche Agronomique, CNRZ, 78350 Jouy-en-Josas, France
Bruno Ribadeau-Dumas
Affiliation:
Institut National de la Recherche Agronomique, CNRZ, 78350 Jouy-en-Josas, France
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Summary

Taking as an example a tryptic hydrolysate of bovine β-casein, it was shown that the method used for the determination of optimal elution conditions for isocratic systems also applied to the separation of complex peptide mixtures by reversed-phase high-performance liquid chromatography with linear gradients. Using a C18 column and the same solvents, successive studies of the influence of flow rate, pH and temperature allowed a satisfactory separation of the sample in less than 30 min. Valuable information on the specificity of the action of trypsin on β-casein was deduced from the yield of the eluted peptides.

Type
Original Articles
Information
Journal of Dairy Research , Volume 53 , Issue 4 , November 1986 , pp. 595 - 600
Copyright
Copyright © Proprietors of Journal of Dairy Research 1986

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References

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