Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-26T08:39:24.751Z Has data issue: false hasContentIssue false
  • English
  • Français

Determination of chymosin and bovine pepsin A activity in combined rennets on the basis of immunochemical inhibition

Published online by Cambridge University Press:  01 June 2009

Eva Beránková ,
Pavel Rauch  and
Jan Káš
Eva Beránková
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Pavel Rauch
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Jan Káš
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Get access Rights & Permissions [Opens in a new window]

Summary

The milk clotting activity of chymosin in combined rennets was determined as the difference between the total and residual activity after specific inhibition of chymosin with the purified immunoglobulin fraction of chymosin antiserum. The milk clotting activity of bovine pepsin A was assessed in a similar manner. In bovine rennets milk clotting activity of chymosin was measured directly after immunochemical inactivation of bovine pepsin A. Any method may be used for the determination of milk clotting activity. High correlation was found between the proposed method, which is simple and rapid to perform, and Chromatographic assay.

Type
Original Articles
Information
Journal of Dairy Research , Volume 56 , Issue 4 , August 1989 , pp. 631 - 637
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Beránková, E., Rauch, P., Káš, J., Hušek, V. & Paluska, E. 1987 Indirect assay of milk clotting activity of Mucor miehei proteinase (Fromase) in combined rennets. Journal of Dairy Research 54 407–412Google Scholar
Beránková, E., Sajdok, J., Rauch, P. & Káš, J. 1988 Determination of chymosin and bovine pepsin content of bovine rennets by high performance liquid chromatography. Netherlands Milk and Dairy Journal 42 337–340Google Scholar
Berridge, N. J. 1955 Purification and assay of rennin. Methods in Enzymology 2 69–77Google Scholar
Collin, J. C., Martin, P., Garnot, P., Ribadeau Dumas, B. & Mocquot, G. 1981 Determination of chymosin and bovine pepsin A in commercial bovine rennets and pepsins. Milchwissenschaft 36 32–35Google Scholar
Garnot, P., Thapon, J. L., Mathieu, C. M., Maubois, J. L. & Ribadeau Dumas, B. 1972 Determination of rennin and bovine pepsins in commercial rennets and abomasal juices. Journal of Dairy Science 55 1641–1650CrossRefGoogle Scholar
Harboe, M. K. 1981 Analysis of milk-clotting enzymes in commercial rennets. Netherlands Milk, and Dairy Journal 35 367–369Google Scholar
Harboe, N. & Ingild, A. 1973 Immunization, isolation of immunoglobulins, estimation of antibody titre. In Manual of Quantitative Immunoelectrophoresis pp. 161–167 (Eds Axelsen, N. H., Krøll, J. and Weeke, B.) Oslo: Universitetsforlaget (Scandinavian Journal of Immunology 2 (Supplement 1))Google Scholar
Laurell, C.-B. 1966 Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Analytical Biochemistry 15 45–52Google Scholar
Martin, P., Collin, J. C., Garnot, P., Ribadeau Dumas, B. & Mocquot, G. 1981 Evaluation of bovine rennets in terms of absolute concentrations of chymosin and pepsin A. Journal of Dairy Research 48 447–456Google Scholar
Mulvihill, D. M. & Fox, P. F. 1977 Selective denaturation of milk coagulants in 5 M-urea. Journal of Dairy Research 44 319–324Google Scholar
Rothe, G. A. L., Axelsen, N. H., Johnk, P. & Foltmann, B. 1976 Immunochemical, Chromatographic, and milk-clotting activity measurements for quantification of milk-clotting enzymes in bovine rennets. Journal of Dairy Research 43 85–95Google Scholar
Rothe, G. A. L., Harboe, M. K. & Martiny, S. C. 1977 Quantification of milk-clotting enzymes in 40 commercial bovine rennets, comparing rocket immunoelectrophoresis with an activity ratio assay. Journal of Dairy Research 44 73–77Google Scholar
Weeke, B. 1973 Rocket immunoelectrophoresis. In Manual of Quantitative Immunoelectrophoresis pp. 37–46 (Eds Axelsen, N. H., Krøll, J. and Weeke, B.) Oslo: Universitetsforlaget (Scandinavian Journal of Immunology 2 (Supplement 1))Google Scholar