Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-23T10:49:59.212Z Has data issue: false hasContentIssue false
  • English
  • Français

Composition, proteolysis indices and coagulating properties of ewe milk as affected by bulk tank somatic cell count

Published online by Cambridge University Press:  24 June 2015

Ana Martí-De Olives ,
María Jesús Navarro-Ríos ,
Joaquín Rubert-Alemán ,
Nemesio Fernández  and
Maria Pilar Molina
Ana Martí-De Olives*
Affiliation:
Departamento de Tecnología Agroalimentaria, Escuela Politécnica Superior de Orihuela, Universidad Miguel Hernández de Elche, 03312 Orihuela, Spain
María Jesús Navarro-Ríos
Affiliation:
Departamento de Tecnología Agroalimentaria, Escuela Politécnica Superior de Orihuela, Universidad Miguel Hernández de Elche, 03312 Orihuela, Spain
Joaquín Rubert-Alemán
Affiliation:
Departamento de Tecnología Agroalimentaria, Escuela Politécnica Superior de Orihuela, Universidad Miguel Hernández de Elche, 03312 Orihuela, Spain
Nemesio Fernández
Affiliation:
Instituto de Ciencia y Tecnología Animal, Universidad Politécnica de Valencia, 46022 Valencia, Spain
Maria Pilar Molina
Affiliation:
Instituto de Ciencia y Tecnología Animal, Universidad Politécnica de Valencia, 46022 Valencia, Spain
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The aim of this study was to assess the effect of ovine bulk tank somatic cell count (BTSCC) on composition, proteose-peptone (p-p) content and casein fractions as indicating parameters for proteolysis and coagulating properties of milk. A total of 97 samples of bulk tank milk from Manchega breed ewe flocks were grouped according to somatic cell count (SCC) into four classes: fewer than 500 000 cells/ml, from 500 000 to 10 00 000 cells/ml, from 10 00 000 to 15 00 000 and more than 15 00 000 cells/ml. The casein : protein ratio and lactose content decreased with BTSCC. Proteolysis increased with BTSCC, causing a drop in β-casein and an increase in the γ-caseins from a concentration of 500 000 cells/ml. Regarding coagulation behaviour, the rennet clotting time (RCT) and firming time (k20) rose from 10 00 000–15 00 000 cells/ml of milk. The results showed that the impairment of milk quality and milk ability to make cheese as affected by intramammary infection (IMI) can be inferred from the bulk tank milk of flocks with poor udder health.

Keywords

Ewe bulk tank milksomatic cell countproteolysismilk coagulating parameters
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 3 , August 2015 , pp. 344 - 349
Copyright
Copyright © Proprietors of Journal of Dairy Research 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Albenzio, M, Caroprese, M, Marino, M, Santillo, A, Taibi, L & Sevi, A 2004 Effects of somatic cell count and stage of lactation on the plasmin activity and cheesemaking properties of ewe milk. Journal of Dairy Science 87 533–542CrossRefGoogle ScholarPubMed
Albenzio, M, Caroprese, M, Santillo, A, Marino, R, Muscio, A & Sevi, A 2005 Proteolytic patterns and plasmin activity in ewe milk as affected by somatic cell count and stage of lactation. Journal of Dairy Research 72 86–92CrossRefGoogle ScholarPubMed
Albenzio, M, Santillo, A, Caroprese, M, d'Angelo, F, Marino, R & Sevi, A 2009 Role of endogenous enzymes in proteolysis of sheep milk. Journal of Dairy Science 92 79–86CrossRefGoogle ScholarPubMed
Albenzio, M, Santillo, A, Caroprese, M, Schena, L, Russo, DE & Sevi, A 2011 Composition, indigenous proteolytic enzymes and coagulating behaviour of ewe milk as affected by somatic cell count. Journal of Dairy Research 78 442–447CrossRefGoogle ScholarPubMed
Andrews, AT 1979 The formation and the structure of some proteose-peptone components. Journal of Dairy Research 46 215–218CrossRefGoogle ScholarPubMed
Arias, R, Oliete, B, Ramón, M, Arias, C, Gallego, R, Montoro, V, Gonzalo, C & Pérez-Guzmán, MD 2012 Long-term study of environmental effects on test-day somatic cell count and milk yield in Manchega sheep. Small Ruminant Research 106 92–97CrossRefGoogle Scholar
Ariznabarreta, A 1999 El recuento celular de la leche como método indirecto de diagnóstico de mastitis subclínicas en el ganado ovino de raza Churra [The SCC as indirect method of subclinic mastitis diagnose in Churra sheep breed]. PhD Thesis, Universidad de León, SpainGoogle Scholar
Auldist, M 2003 Effect on processing characteristics. In Encyclopedia of Dairy Sciences, Vol 3. pp. 2002–2007 (Eds Roginski, H, Fuquay, JW & Fox, PF). Bodmin, Cornwall, UK: MPG Books LtdGoogle Scholar
Auldist, MJ, Coats, S, Sutherlands, BJ, Mayes, JJ, McDowell, GH & Rogers, GL 1996 Effects of somatic cell count and stage of lactation on raw milk composition and the yield and quality of Cheddar cheese. Journal of Dairy Research 63 269–280CrossRefGoogle ScholarPubMed
Berthelot, X, Lagriffoul, G, Concordet, D, Barillet, F & Bergonier, D 2006 Physiological and pathological thresholds of somatic cell counts in ewe milk. Small Ruminant Research 62 27–31CrossRefGoogle Scholar
Bianchi, L, Bolla, A, Budelli, E, Caroli, A, Casoli, C, Pauselli, M & Duranti, E 2004 Effect of udder health status and lactation phase on the characteristics of Sardinian ewe milk. Journal of Dairy Science 87 2401–2408CrossRefGoogle ScholarPubMed
Burriel, AR 1997 Dynamics of intramammary infection in the sheep caused by coagulase-negative staphylococci and its influence on udder tissue and milk composition. Veterinary Record 140 419–423CrossRefGoogle ScholarPubMed
Delacroix-Buchet, A, Barillet, F & Lagriffoul, G 1994 Characterization of cheese making properties of individual Lacaune ewes’ milk samples with Formagraph. Lait 74 173–186CrossRefGoogle Scholar
Duranti, E & Casoli, C 1991 Variations in the nitrogen composition and in the lactodynamographic characteristics of ewes’ milk in relation to somatic cell content. Zootecnica e Nutrizione Animale 17 99–105Google Scholar
European Union 1994 Council Directive 94/71/EEC of 13 December 1994 amending Directive 92/46/EEC laying down the health rules for the production and placing on the market of raw milk, heat-treated milk and milk-based products. Official Journal of European Community L368 33–37Google Scholar
Gonzalo, C, Tardáguila, JA, Ariznabarreta, A, Romeo, M, Montoro, V, Pérez-Guzmán, MD & Marco, JC 2000 Recuentos de células somáticas en el ganado ovino lechero y estrategias de control. Situación en España [Somatic cells count in sheep milk and control strategies]. Ovis 66 21–27Google Scholar
Gonzalo, C, Ariznabarreta, A, Carriedo, JA & San Primitivo, F 2002 Mammary pathogens and their relationship to somatic cell count and milk yield losses in dairy ewes. Journal of Dairy Science 85 1460–1467CrossRefGoogle ScholarPubMed
Gonzalo, C, Carriedo, JA, Blanco, MA, Beneitez, E, Juarez, MT, De La Fuente, LF & San Primitivo, F 2005 Factors of variation influencing bulk tank somatic cell count in dairy sheep. Journal of Dairy Science 88 969–974CrossRefGoogle ScholarPubMed
Gonzalo, C, Carriedo, JA, Beneitez, E, Juarez, MT, De La Fuente, LF & San Primitivo, F 2006 Short Communication: bulk tank total bacterial count in dairy sheep: factors of variation and relationship with somatic cell count. Journal of Dairy Science 89 549–552CrossRefGoogle ScholarPubMed
Gonzalo, C, Carriedo, JA, García-Jimeno, MC, Pérez-Bilbao, M & De la Fuente, LF 2010 Factors influencing variation of bulk milk antibiotic residue occurrence, somatic cell count, and total bacterial count in dairy sheep flocks. Journal of Dairy Science 93 1587–1595CrossRefGoogle ScholarPubMed
IDF 1995 Counting somatic cells in milk: International IDF standard 148A. In Newsletters of the International Dairy Federation No 142-Mastitis Newsletter 20. pp. 14–25. Schällibaum, M ed. International Dairy Federation, Brussels, Belgium.Google Scholar
ISO-IDF 2008 Milk and milk products — Guidance on sampling. International standard ISO 707-IDF 50, pp. 40. International Organization of Standardization, Geneva, Switzerland.Google Scholar
Klei, L, Yun, J, Sapru, A, Lynch, J, Barbano, D, Sears, P & Galton, D 1998 Effects of milk somatic cell count on Cottage cheese yield and quality. Journal of Dairy Science 81 1205–1213CrossRefGoogle ScholarPubMed
Leitner, G, Chaffer, M, Caraso, Y, Ezra, E, Kababea, D, Winkler, M, Glickman, A & Saran, A 2003 Udder infection and milk somatic cell count, NAGase activity and milk composition – fat, protein and lactose – in Israeli-Assaf and Awassi sheep. Small Ruminant Research 49 157–164CrossRefGoogle Scholar
Leitner, G, Chaffer, M, Shamay, A, Shapiro, F, Merin, U, Ezra, E, Saran, A & Silanikove, N 2004 Changes in milk composition as affected by subclinical mastitis in sheep. Journal of Dairy Science 87 46–52CrossRefGoogle ScholarPubMed
Leitner, G, Silanikove, N & Merin, U 2008 Short communication: estimate of milk and curd yield loss of sheep and goats with intrammamary infection and its relation to somatic cell count. Small Ruminant Research 74 221–225CrossRefGoogle Scholar
Le Roux, Y, Colin, O & Laurent, F 1995 Proteolysis in samples of quarters milk with varying somatic cell counts. 1. Comparison of some indicators of endogenous proteolysis in milk. Journal of Dairy Science 78 1289–1297CrossRefGoogle ScholarPubMed
Martí-De Olives, A, Le Roux, Y, Rubert-Alemán, J, Peris, C & Molina, MP 2011 Short communication: effect of subclinical mastitis on proteolysis in ovine milk. Journal of Dairy Science 94 5369–5374CrossRefGoogle ScholarPubMed
Martí-De Olives, A, Díaz, JR, Molina, MP & Peris, C 2013 Quantification of milk yield and composition changes as affected by subclinical mastitis during the current lactation in sheep. Journal of Dairy Science 96 7698–7708CrossRefGoogle ScholarPubMed
McMahon, DJ & Brown, RJ 1982 Evaluation of Formagraph for comparing rennet solutions. Journal of Dairy Science 65 1639–1642CrossRefGoogle Scholar
Paape, MJ, Wiggans, GR, Bannerman, DD, Thomas, DL, Sanders, AH, Contreras, A, Moroni, P & Miller, RH 2007 Monitoring goat and sheep milk somatic cell counts. Small Ruminant Research 68 114–125CrossRefGoogle Scholar
Papoff, CM, Law, AJR, Dalgleish, DG & Campus, RL 1993 Determination of the composition of ovine casein by anion-exchange FPLC. Scienza e tecnica lattiero-casearia 44 273–291Google Scholar
Park, YW 2007 Rheological characteristics of goat and sheep milk. Small Ruminant Research 68 73–87CrossRefGoogle Scholar
Pellegrini, O, Florent, R, Rivemale, M & Barillet, F 1997 Renneting properties of milk from individual ewes: influence of genetic and non genetic variables and relationship with physicochemical characteristics. Journal of Dairy Research 64 355–366CrossRefGoogle Scholar
Pinto, G, Caira, S, Nicolai, MA, Mauriello, R, Cuollo, M, Pirisi, A, Piredda, G, Chianese, L & Addeo, F 2013 Proteolysis and partial dephosphorylation of casein are affected by high somatic cell counts in sheep milk. Food Research International 53 510–521CrossRefGoogle Scholar
Pirisi, A, Piredda, G, Podda, F & Pintus, S 1996 Effect of somatic cell count on sheep milk composition and cheesemaking properties. In Somatic Cells and Milk of Small Ruminants. EAAP Publication No 77. pp. 245–252 (Ed. Rubino, R). Wageningen, The Netherlands: Wageningen Pers Publ.Google Scholar
Pirisi, A, Gaspard, CE, Piredda, G, Jaubert, A & Ledda, A 1999 Relation entre CCS et les caractéristiques du lait de brebis et de chèvre [Relationship between SCC and the characteristics of sheep and goat milk]. In Milking and Milk Production of Dairy Sheep and Goats. EAAP Publication No 95. pp. 495–500 (Eds Barillet, F & Zervas, NP). Wageningen, The Netherlands: Wageningen Pers Publ.Google Scholar
Raynal-Ljutovac, K, Pirisi, A, De Cremoux, R & Gonzalo, C 2007 Somatic cells and goat and sheep milk: analytical, sanitary, productive and technological aspects. Small Ruminant Research 68 126–144CrossRefGoogle Scholar
Revilla, I, Rodríguez-Nogales, GM & Vivar-Quintana, AM 2007 Proteolysis and texture of hard ewe's milk cheese during ripening as affected by somatic cell count. Journal of Dairy Research 74 127–136CrossRefGoogle Scholar
Revilla, I, Rodríguez-Nogales, JM & Vivar-Quintana, AM 2009 Effect of somatic cell counts on ewes’ milk protein profile and cheese-making properties in different sheep breeds reared in Spain. Journal of Dairy Research 76 210–215CrossRefGoogle ScholarPubMed
Ribadeau-Dumas, R & Grappin, R 1989 Milk protein analysis. Lait 69 357–366CrossRefGoogle Scholar
Rowland, SJ 1938 The precipitation of the protein in milk, I caseins, II total proteins, III globulins, IV albumin and proteose-peptone. Journal of Dairy Research 9 31–41CrossRefGoogle Scholar
SAS Institute 2011 SAS User's Guide: Statistics, Version 9.2 ed. SAS Inst. Inc., Cary, NCGoogle Scholar
Sevi, A, Albenzio, M, Taibi, L, Dantone, D, Massa, S & Annicchiarico, G 1999 Changes of somatic cell count through lactation and their effects on nutritional, renneting and bacteriological characteristics of ewe's milk. Advances in Food Science 21 122–127Google Scholar
Silanikove, N, Merin, U & Leitner, G 2006 Physiological role of endogenous milk enzymes: an overview of an evolving picture. International Dairy Journal 16 533–545CrossRefGoogle Scholar
Storry, JE, Grandison, AS, Millard, D, Owen, AJ & Ford, GD 1983 Chemical composition and coagulation properties of renneted milks from different breeds and species of ruminant. Journal of Dairy Research 50 215–229CrossRefGoogle Scholar