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Comparison of antioxidant defence parameters in colostrum and milk between Berrichon du Cher ewes and Uhrusk ewes

Published online by Cambridge University Press:  07 January 2010

Justyna Lipko-Przybylska ,
Edyta Albera  and
Marta Kankofer
Justyna Lipko-Przybylska
Affiliation:
Department of Animal Biochemistry and Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20–033 Lublin, ul. Akademicka 12, Poland
Edyta Albera
Affiliation:
Department of Animal Biochemistry and Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20–033 Lublin, ul. Akademicka 12, Poland
Marta Kankofer*
Affiliation:
Department of Animal Biochemistry and Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20–033 Lublin, ul. Akademicka 12, Poland
*
*For correspondence; e-mail: [email protected]
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Abstract

The aim of the study was to evaluate the profile of antioxidant parameters in ewes' colostrum and milk in relation to breed during 5 d post partum. Total antioxidant capacity (TAC) was analysed and the activity of the enzymic antioxidants, glutathione peroxidase (GSH-Px) and glutathione transferase (GSH-Tr), as well as the concentration of the non-enzymic antioxidants, vitamin C, vitamin A and β-carotene, were measured. Samples were collected from healthy animals belonging to two ewe breeds: Berrichon du Cher (n=15) and Uhrusk (n=15) kept in the Podlasie Province (Poland). Colostrum was sampled directly after parturition, as well as after 12, 24 and 48 h later and milk was sampled 5 d after parturition. Colostrum and milk for the evaluation of all parameters except for vitamin A and β-carotene were centrifuged, and the supernatant was used for further analysis. Spectrophotometric methods were used for biochemical measurements. The results showed dynamic changes of antioxidative parameters within the time period examined. TAC values and GSH-Px activity increased significantly during the experiment. GSH-Tr activity showed a similar tendency in Uhrusk ewes but an opposite relationship in Berrichon du Cher. Concentrations of examined vitamins followed the increasing trends noticed in the activities of antioxidative enzymes. Moreover, differences between breeds in the evaluated parameters were detected; these differences were not unequivocal however. The results are also a source of not previously published physiological antioxidant profile in colostrum and milk of ewes over the post-partum period.

Keywords

Antioxidantscolostrumewes
Type
Research Article
Information
Journal of Dairy Research , Volume 77 , Issue 1 , February 2010 , pp. 117 - 122
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

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References

Al-Gubory, KH, Bolifraud, P & Garrel, C 2008 Regulation of key antioxidant enzymatic systems in the sheep endometrium by ovarian steroids. Endocrinology 149 4428–4434CrossRefGoogle ScholarPubMed
Antunovic, Z, Steiner, Z, Sencic, D, Mandic, M & Klapec, T 2001 Changes in ewe milk composition depending on lactation stage and feeding season. Czech Journal of Animal Science 46 75–82Google Scholar
Benzie, IF & Strain, JJ 1996 The ferric reducing ability of plasma (FRAP) as a measure of ‘antioxidant power’: The FRAP assay. Analytical Biochemistry 239 70–76CrossRefGoogle ScholarPubMed
Bertoni, G, Maianti, MG & Cappa, V 1984 Changes in the lipidic and glucydic metabolism during the last period of pregnancy and early lactation in cows. In Proceedings of the Italian Society of Buiatric 16 223–236Google Scholar
Bhattacharya, ID, Picciano, MF & Milner, JA 1988 Characteristics of human milk glutathione peroxidase. Biology of Trace Element Research 18 59–70Google Scholar
Blum, JW, Hadorn, U, Sallmann, HP & Schuep, W 1997 Delaying colostrum intake by one day impairs plasma lipid, essential fatty acid, carotene, retinol and α-tocopherol status in neonatal calves. Journal of Nutrition 127 2024–2029Google Scholar
Blum, JW 2006 Nutritional physiology of neonatal calves. Journal of Animal Physiology and Animal Nutrition 90 1–86Google Scholar
Capper, JL, Wilkinson, RG, Kasapidou, E, Pattinson, SE, Mackenzie, AM & Sinclair, LA 2005 The effect of dietary vitamin E and fatty acid supplementation of pregnant and lactating ewes on placental and mammary transfer of vitamin E to the lamb. British Journal of Nutrition 93 549–557CrossRefGoogle Scholar
Dębski, B, Piccano, MF & Milner, JA 1987 Selenium content and distribution of human, cow and goat milk. Journal of Nutrition 117 1091–1097Google Scholar
Fadel, I, Owen, JB, Kassem, R & Juha, H 1989 A note on the milk composition of Awassi ewes. Animal Production 48 606–610Google Scholar
Fantova, M & Zıkova, E 1988 Spectrum of minerals in the colostrum of ewes. Zivoczisna Vyroba 33 635–642Google Scholar
Fürll, B, Wilken, H & Fürll, M 2003 Water-soluble antioxidant in ewes during their late pregnancy. Acta Veterinaria Scandinavica 98 Suppl 302Google Scholar
Halliwell, B & Gutteridge, JM 1995 The definition and measurement of antioxidants in biological systems. Free Radical Biology and Medicine 18 125–126Google Scholar
Halliwell, B 2006 Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiology 141 312–322Google Scholar
Hojo, Y 1986 Seqential study of glutathione peroxidase and selenium contents of human milk. Science of the Total Envirioment 52 83–91Google Scholar
Hoolbrook, JJ & Hicks, CL 1978 Variation of superoxide dismutase of bovine milk. Journal of Dairy Science 61 1072–1077Google Scholar
Kankofer, M & Lipko-Przybylska, J 2008 Physiological antioxidative/oxidative balance in bovine colostrum and mature milk. Acta Veterinaria Beograd 58 231–239Google Scholar
Kasapovice, J, Pejice, S, Mladenovice, M, Radlovice, N & Pajovice, SB 2005 Superoxide dismutase activity in colostrum, transitional and mature human milk. Turkish Journal of Pediatrics 47 343–347Google Scholar
Kiyosawa, I, Matuyama, J, Nyui, S, Yoshida, K 1993 Cu, Zn- and Mn-superoxide dismutase concentration in human colostrums and mature milk. Bioscience Biotechnology and Biochemistry 57 676–677Google Scholar
Kracmar, S, Kuchtik, J, Baran, M, Varadyova, Z, Kracmarova, E, Gajdusek, S, & Jelinek, P 2005 Dynamics of changes in contents of organic and inorganic substances in sheep colostrum within the first 72 h after parturition. Small Ruminant Research 56 183–188Google Scholar
Mohamed, HE, Mousa, HM & Beynen, AC 2005 Ascorbic acid concentrations in milk from Sudanese camels. Journal of Animal Physiology and Animal Nutrition 89 35–37Google Scholar
Morris, CA, Knight, TW, Newman, SAN, Hickey, SM, Death, AF, O`Neill, KT & Ridland, M 2002 Genetic studies of carotenoid concentration in the plasma and milk of New Zealand dairy Cattle. New Zealand Journal of Agricultural Research 45 27–33Google Scholar
Noziere, P, Graulet, B, Lucas, A, Martin, B, Grolier, P & Doreau, M 2006 Carotenoids for ruminants: from forages to dairy products. Animal Feed Science and Technology 131 418–450CrossRefGoogle Scholar
Omaye, ST, Turnbull, JD & Sauberlich, HE 1979 Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. Methods in Enzymology 62 3–11CrossRefGoogle ScholarPubMed
Paglia, DE & Valentine, WN 1967 Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine 70 15–169Google Scholar
Pajovic, SB & Saicic, ZS 2008 Modulation of antioxidant enzyme activities by sexual steroid hormones. Physiological Research 57 801–811CrossRefGoogle ScholarPubMed
Park, YW, Juarez, M, Ramos, M & Haenlein, GFW 2007 Physicochemical characteristics of goat and sheep milk. Small Ruminant Research 68 88–113Google Scholar
Piccione, G, Casella, S, Assenza, A, Fazio, F & Caola, G 2008 Evaluation of serum homocysteine and oxidative stress during lactation in ewes. Czech Journal of Animal Sciences 53 462–465Google Scholar
Pinelli-Saavedra, A & Scaife, JR 2005 Pre- and postnatal transfer of vitamins E and C to piglets in sows supplemented with vitamin E and vitamin C. Livestock Production Science 97 231–240Google Scholar
Przybylska, J, Albera, E & Kankofer, M 2007 Antioxidants in bovine colostrum. Reproduction in Domestic Animals 42 402–409Google Scholar
Rice-Evans, CA, Diplock, AT & Symons, MCR 1991 Techniques in Free Radical Research. Amsterdam, London, New York, Tokyo: ElsevierGoogle Scholar
Savice, D, Vojinovice, J, Zvezdanovice, L, Cosice, V & Savice, V 2005 Importance of breast-feeding in antioxidant defence. Srpski Arhiv za Celokupno Lekarstvo 133 108–112CrossRefGoogle Scholar
Snitynskyj, VV, Antoniak, HL & Bershadskyi, VI 1996 The age-related changes in the antioxidant system enzymes of the erythroid cells of swine at the early stages of postnatal development. Fiziologicheski Zhurnal 42 19–25Google Scholar
Storey, KB 1996 Oxidative stress: animal adaptations in nature. Brazilian Journal of Medical Biology Research 29 1715–1733Google Scholar
Suzuki, J & Katoh, N 1990 A simple and cheap method for measuring serum vitamin A in cattle using only a spectrophotometer. Japanese Journal of Veterinary Science 52 1281–1283Google Scholar
Yang, A & Tume, RK 1993 A comparison of beta-carotene-splitting activity isolated from intestinal mucosa of pasture-grazed sheep, goats and cattle. Biochemistry and Molecular Biology International 30 209–217Google Scholar
Yang, A, Larsen, TW & Tume, RK 1992 Carotenoid and retinol concentrations in serum, adipose-tissue and liver and carotenoid transport in sheep, goats and cattle. Australian Journal of Agricultural Research 43 1809–1817Google Scholar
Zhao, J, Liu, XJ, Ma, JW & Zheng, RL 2004 DNA damage in healthy neonate. Early Human Development 77 89–98Google Scholar