Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-06T01:51:11.777Z Has data issue: false hasContentIssue false

Chemical characterization of milk concentrated by ultrafiltration

Published online by Cambridge University Press:  01 June 2009

Margaret L. Green
Affiliation:
*National Institute for Research in Dairying, (University of Reading), Shinfield, Reading RG2 9AT, UK,
K. John Scott
Affiliation:
*National Institute for Research in Dairying, (University of Reading), Shinfield, Reading RG2 9AT, UK,
Malcolm Anderson
Affiliation:
*National Institute for Research in Dairying, (University of Reading), Shinfield, Reading RG2 9AT, UK,
Mary C. A. Griffin
Affiliation:
*National Institute for Research in Dairying, (University of Reading), Shinfield, Reading RG2 9AT, UK,
Frank A. Griffin
Affiliation:
*National Institute for Research in Dairying, (University of Reading), Shinfield, Reading RG2 9AT, UK,

Summary

Whole milks concentrated 1·5–4-fold and acidified and citrated milks concentrated 2·8-fold by ultrafiltration at 50 °C were analysed for chemical changes relevant to further processing, storage or nutrition. Fat and protein were entirely retained in the concentrate. The retention of water-soluble vitamins, Ca, Mg, phosphate and trace minerals depended on the proportion bound to the protein. Ascorbic acid was rapidly destroyed during concentration. Because of the differential retention of nitrogenous components, protein comprised a progressively higher proportion of the total N as the milk became more concentrated. No denaturation of whey protein or disruption of casein micelles was detected during concentration of whole milk, but some solubilization of the casein occurred after citration. Reduction of fat globule size occurred early in the concentration process, damage to the fat globule membrane was indicated and the milk became more susceptible to lipolysis. Apart from a tendency for preacidified or precitrated concentrates to gel, no change in the susceptibility of the milks to heat damage was detected.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1984

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Anderson, M. 1982 Stability of lipoprotein lipase activity in bovine milk. Journal of Dairy Research 49 231237.CrossRefGoogle Scholar
Anderson, M. & Andrews, A. T. 1977 Progressive changes in individual milk protein concentrations associated with high somatic cell counts. Journal of Dairy Research 44 223235CrossRefGoogle ScholarPubMed
British Standards Institution 1963 Methods for the chemical analysis of liquid milk and cream. B.S. no. 1741Google Scholar
British Standards Institution 1969 Gerber method for the determination of fat in milk and milk products. B.S. no. 696, part 2Google Scholar
Brown, J. C. & Pusey, P. N. 1974 Measurement of diffusion coefficients of polydisperse solutes by photon correlation spectroscopy. Journal of Physics D 7 L31L35CrossRefGoogle Scholar
Brule, G. & Fauquant, J. 1981 Mineral balance in skim-milk and milk retentate: effect of physicochemical characteristics of the aqueous phase. Journal of Dairy Research 48 9197CrossRefGoogle Scholar
Brule, G., Maubois, J. L. & Fauquant, J. 1974 [Contents of mineral elements in products from ultrafiltered milk.] Lait 54 600615CrossRefGoogle Scholar
Ceriotti, G. 1971 Ultramicrodetermination of plasma urea by reaetion with diacetylmonoxime-antipyrine without deproteinization. Clinical Chemistry 17 400402CrossRefGoogle ScholarPubMed
Chandan, R. C. & Shahani, K. M. 1964 Milk lipases: a review. Journal of Dairy Science 47 471480CrossRefGoogle Scholar
Conway, E. J. 1957 Microdiffusion Analysis and Volumetric Error, 4th edn, pp. 9193. London: Crosby Lockwood & Son LtdGoogle Scholar
Davies, D. T. & White, J. C. D. 1960 The use of ultrafiltration and dialysis in isolating the aqueous phase of milk and in determining the partition of milk constituents between the aqueous and disperse phases. Journal of Dairy Research 27 171190CrossRefGoogle Scholar
Deeth, H. C. & Fitz-Gerald, C. H. 1978 Effects of mechanical agitation of raw milk on the milk-fat globule in relation to the level of induced lipolysis. Journal of Dairy Research 45 373380CrossRefGoogle Scholar
De Rham, O. & Andrews, A. T. 1982 The roles of native milk proteinase and its zymogen during proteolysis in normal bovine milk. Journal of Dairy Research 49 577585CrossRefGoogle ScholarPubMed
Ernstrom, C. A., Sutherland, B. J. & Jameson, G. W. 1980 Cheese base for processing. A high yield product from whole milk by ultrafiltration. Journal of Dairy Science 63 228234CrossRefGoogle Scholar
Evans, E. W. & Glover, F. A. 1978 Potential for membrane processes in dairy processing. 20th International Dairy Congress, Paris E 647648Google Scholar
Ford, J. E. 1967 The influence of the dissolved oxygen in milk on the stability of some vitamins towards heating and during subsequent exposure to sunlight. Journal of Dairy Research 34 239247CrossRefGoogle Scholar
Ford, J. E., Salter, D. N. & Scott, K. J. 1969 The folate-binding protein in milk. Journal of Dairy Research 36 435446CrossRefGoogle Scholar
Fukuwatari, Y., Tomita, M., Tamura, Y., Mizota, T., Kiyosawa, I. & Kuboyama, M. 1982 Membrane permeability to trace metal elements in whole milk by ultrafiltration. 21st International Dairy Congress, Moscow 1 (2) p. 447Google Scholar
Glover, F. A. 1971 Concentration of milk by ultrafiltration and reverse osmosis. Journal of Dairy Research 38 373379CrossRefGoogle Scholar
Green, M. L., Hobbs, D. G. & Morant, S. V. 1978 Intermicellar relationships in rennet-treated separated milk. I. Preparation of representative electron micrographs. Journal of Dairy Research 45 405411CrossRefGoogle Scholar
Green, M. L., Marshall, R. J. & Glover, F. A. 1983 Influence of homogenization of concentrated milks on the structure and properties of rennet curds. Journal of Dairy Research 50 341348CrossRefGoogle Scholar
Gregory, M. E., Ford, J. E. & Kon, S. K. 1952 A vitamin B12-binding factor in sow's milk. Biochemical Journal 51 xxixGoogle Scholar
Hartman, A. M. & Dryden, L. P. 1974 The vitamins in milk and milk products. In Fundamentals of Dairy Chemistry, 2nd edn pp. 325401 (Eds Webb, B. H., Johnson, A. H. and Alford, J. A.) Westport, CT: Avi Publishing Co. Inc.Google Scholar
Hill, A. W., Hibbitt, K. G. & Davies, J. 1982 Particles in bulk milk capable of causing falsely high electronic cell counts. Journal of Dairy Research 49 171177CrossRefGoogle Scholar
Hillier, R. M. 1976 The quantitative measurement of whey proteins using polyacrylamide-gel electro phoresis. Journal of Dairy Research 43 259265CrossRefGoogle Scholar
Hillier, R. M., Lyster, R. L. J. & Cheeseman, G. C. 1979 Thermal denaturation of α-lactalbumin and β-lactoglobulin in cheese whey: effect of total solids concentration and pH. Journal of Dairy Research 46 103111.CrossRefGoogle Scholar
Hurrell, R. F. 1980 Interaction of food components during processing. In Food and Health: Science and Technology pp. 369388 (Eds Birch, G. G. and Parker, K. J.) London: Applied ScienceCrossRefGoogle Scholar
Hurrell, R. F. & Carpenter, K. J. 1975 The use of three dye-binding procedures for the assessment of heat damage to food proteins. British Journal of Nutrition 33 101115CrossRefGoogle ScholarPubMed
Koops, J. & Klomp, H. 1977 Rapid colorimetric determination of free fatty acids (lipolysis) in milk by the copper soap method. Netherlands Milk and Dairy Journal 31 5674Google Scholar
Kreula, M., Kiviniemi, L., Vuorinen, E. & Heikonen, M. 1974 The design of an ultrafiltration process for whey and skim milk. Milchwissenschaft 29 129137Google Scholar
Lang, C. A. 1958 Simple microdetermination of Kjeldahl nitrogen in biological materials. Analytical Chemistry 30 16921694CrossRefGoogle Scholar
Lyster, R. L. J. 1964 The free and masked sulphydryl groups of heated milk and milk powder and a new method for their determination. Journal of Dairy Research 31 4151CrossRefGoogle Scholar
Mann, E. J. 1982 Ultrafiltration of milk for cheesemaking. Dairy Industries International 47 (12) 1112Google Scholar
Matthews, M. E., Amundson, C. H. & Hill, C. G. 1976 Changes in distribution of nitrogenous fractions of Cheddar cheese whey during ultrafiltration. Journal of Dairy Science 59 10331041CrossRefGoogle Scholar
Maubois, J. L. & Mocquot, G. 1975 Application of membrane ultrafiltration to preparation of various types of cheese. Journal of Dairy Science 58 10011007CrossRefGoogle Scholar
Rowland, S. J. 1938 The determination of the nitrogen distribution in milk. Journal of Dairy Research 9 4246CrossRefGoogle Scholar
Salter, D. N., Scott, K. J., Slade, H. & Andrews, P. 1981 The preparation and properties of folate-binding protein from cow's milk. Biochemical Journal 193 469476CrossRefGoogle ScholarPubMed
Scott, K. J., Bishop, D. R., Zechalko, A., Edwards-Webb, J. D., Jackson, P. A. & Scuffam, D. 1984 Nutrient content of liquid milk. I. Vitamins A, D3, C and of the B complex in pasteurized bulk liquid milk. Journal of Dairy Research 51 3750.CrossRefGoogle Scholar
Simonsen, D. G., Wertman, M., Westover, L. M. & Mehl, J. W. 1946 The determination of serum phosphate by the molybdivanadate method. Journal of Biological Chemistry 166 747755CrossRefGoogle ScholarPubMed
Slack, A. W., Amundson, C. H. Jr, Hill, C. G. & Jorgensen, N. A. 1982 a On-farm ultrafiltration of milk: Part 1 –Technical feasibility studies. Process Biochemistry 17 (4) 611Google Scholar
Slack, A. W., Amundson, C. H. Jr & Hill, C. G. 1982 b On-farm ultrafiltration of milk: Part 2 –Economic analysis. Process Biochemistry 17 (5) 2325, 30Google Scholar
Spies, J. R. 1957 Colorimetric procedures for amino acids. Methods in Enzymology 3 467477CrossRefGoogle Scholar
Walstra, P. 1979 The voluminosity of bovine casein micelles and some of its implications. Journal of Dairy Research 46 317323CrossRefGoogle ScholarPubMed
White, J. C. D. & Davies, D. T. 1963 The determination of citric acid in milk and milk sera. Journal of Dairy Research 30 171189CrossRefGoogle Scholar