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Characterization and response to mitogens of mammary lymphocytes from the bovine dry-period secretion

Published online by Cambridge University Press:  01 June 2009

Carlos Concha ,
Olof Holmberg  and
Bror Morein
Carlos Concha
Affiliation:
Department of Biological Products, National Veterinary Institute, S-104 05 Stockholm, Sweden
Olof Holmberg
Affiliation:
Department of Bacteriology, National Veterinary Institute, S-750 07 Uppsala, Sweden
Bror Morein
Affiliation:
Department of Biological Products, National Veterinary Institute, S-104 05 Stockholm, Sweden
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Summary

From bovine mammary secretion during the dry period, the total number of cells was between 1·2 and 5·9 Ǻ 106/ml. A mean of 35% of these cells were classified as lymphocytes and ∼ 85% of them could be isolated by the Ficoll-isopac method. Centrifugation separated 6% of the cells into the fat; 5% of them were lymphocytes. About 47% of the lymphocytes bound Helix pomatia agglutinin, a T-cells marker, while the proportion of Ig-bearing cells was ∼ 28%. The mammary lymphocytes were stimulated by the lectins phytohaemagglutinin, pokeweed mitogen, concanavalin A and by lipopolysaccharide from Salmonella typhimurium. The stimulation indices of mammary lymphocytes were generally lower than those for peripheral blood lymphocytes from the same animals. The background values, i.e. counts/min of lymphocytes incubated without mitogen, were often higher for lymphocytes isolated from mammary secretion than from blood.

Type
Original Articles
Information
Journal of Dairy Research , Volume 47 , Issue 3 , October 1980 , pp. 305 - 311
Copyright
Copyright © Proprietors of Journal of Dairy Research 1980

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References

REFERENCES

Ahlstedt, S., Carlsson, B., Hanson, L.Ǻ. & Goldblum, R. M. (1975). Scandinavian Journal of Immunology 4, 535–539.CrossRefGoogle Scholar
Beer, A. E., Billingham, R. E. & Head, J. (1974). Journal of Investigative Dermatology 63, 65–74.CrossRefGoogle Scholar
Bøyum, A. (1968). Scandinavian Journal of Clinical and Laboratory Investigation 21, suppl. 97.Google Scholar
Butler, J. E., Maxwell, C. F., Pierce, C. S., Hylton, M. B., Asofsky, R. & Kiddy, C. A. (1972). Journal of Immunology 109, 38–46.CrossRefGoogle Scholar
Concha, C., Holmberg, O. & Morein, B. (1978). Journal of Dairy Research 45, 287–290.CrossRefGoogle Scholar
Crago, S. S., Prince, S. J. & Mestecky, J. (1979). Protides of Biological Fluids 27, 585–588.Google Scholar
Diaz-Jouanen, E. & Williams, R. C. Jr (1974). Clinical Immunology and Immunopathology 3, 248–255.CrossRefGoogle Scholar
Goldblum, R. M., Ahlstedt, S., Carlsson, B., Hanson, L.Ǻ., Jodal, U., Lidin-Janson, G. & Sohl-ǺKerlund, A. (1975). Nature 257, 797–799.CrossRefGoogle Scholar
Head, J. R. & Beer, A. E. (1978). In Lactation: A comprehensive Treatise, vol. 4, pp. 337–364. (Ed. Larson, B. L..) New York: Academic Press.Google Scholar
Hübler, C., Heeschen, W., Hamann, J. & Tolle, A. (1978). Milchwissenschaft 33, 101–105.Google Scholar
Johnson, K. & Morein, B. (1977). Research in Veterinary Science 22, 83–85.CrossRefGoogle Scholar
Junge, U., Hoekstra, J., Wolfe, L. & Deinhardt, F. (1970). Clinical and Experimental Immunology 7, 431–437.Google Scholar
Klareskog, L., Forsum, U. & Peterson, P. A. (1979). European Journal of Immunology. (In press.)Google Scholar
Lascelles, A. K. (1979). Journal of Dairy Science 62, 154–160.CrossRefGoogle Scholar
Ling, N. R. & Kay, J. E. (1975). Lymphocyte Stimulation, 2nd edn, p. 103. Amsterdam: North-Holland.Google Scholar
Morein, B., Hellström, U., Axelsson, L. G., Johansson, C. & Hammarström, S. (1979). Veterinary Immunology and Immunopathology 1, 27–36.CrossRefGoogle Scholar
Murillo, G. J. & Goldman, A. S. (1970). Pediatric Research 4, 71–75.CrossRefGoogle Scholar
Norcross, N. L. (1971). Journal of Dairy Science 54, 1880–1885.CrossRefGoogle Scholar
Ogra, S. S. & Ogra, P. L. (1978). Journal of Pediatrics 92, 550–555.CrossRefGoogle Scholar
Parmely, M. J. & Beer, A. E. (1977). Journal of Dairy Science 60, 655–665.CrossRefGoogle Scholar
Parmely, M. J., Beer, A. E. & Billinoham, R. E. (1976). Journal of Experimental Medicine 144, 358–370.CrossRefGoogle Scholar
Prescott, S. C. & Breed, R. S. (1910). Journal of Infectious Diseases 7, 632–640.CrossRefGoogle Scholar
Reiter, B. (1978). Journal of Dairy Research 45, 131–147.CrossRefGoogle Scholar
Rosenthal, A. (1978). Immunological Review 40, 136–152.CrossRefGoogle Scholar
Schlesinger, J. J. & Covelli, H. D. (1977). Lancet ii, 529–532.CrossRefGoogle Scholar
Smith, C. W. & Goldman, A. S. (1968). Pediatric Research 2, 103–109.CrossRefGoogle Scholar
Smith, J. W. & Schultz, R. D. (1977). Cellular Immunology 29, 165–173.CrossRefGoogle Scholar
Watson, D. L. (1976). Immunology 31, 159–165.Google Scholar
Watson, D. L. & Lascelles, A. K. (1975). Research in Veterinary Science 18, 182–185.CrossRefGoogle Scholar
Wiman, K., Curman, B.,Trägårdh, L. & Peterson, P. A. (1979). European Journal of Immunology 9, 190–195.CrossRefGoogle Scholar
Yokomizo, Y. & Norcross, N. L. (1978). American Journal of Veterinary Research 39, 511–516.Google Scholar