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Changes in the microflora of La Serena ewes' milk cheese during ripening

Published online by Cambridge University Press:  01 June 2009

Buensuceso Fernandez Del Pozo
Affiliation:
Departamento de Bioquímica y Microbiología, INIA, Apartado 8111, Madrid 28080, Spain
Pilar Gaya
Affiliation:
Departamento de Bioquímica y Microbiología, INIA, Apartado 8111, Madrid 28080, Spain
Margarita Medina
Affiliation:
Departamento de Bioquímica y Microbiología, INIA, Apartado 8111, Madrid 28080, Spain
M. Asunción Rodríguez-Marín
Affiliation:
Departamento de Bioquímica y Microbiología, INIA, Apartado 8111, Madrid 28080, Spain
Manuel Nuñez
Affiliation:
Departamento de Bioquímica y Microbiología, INIA, Apartado 8111, Madrid 28080, Spain

Summary

The microflora of ten batches of La Serena cheese, made from raw milk of Merino ewes and using vegetable rennet as coagulant, was monitored throughout a 60 d ripening period. After 15 d, lactic acid bacteria predominated in the interior of the cheese, whilst lactic acid bacteria and lactic acid-utilizing species of yeasts and moulds predominated on the cheese surface. Coagulase-positive staphylococci were not detected in the interior or on the surface of any of the cheeses after 45 d and faecal coliforms were not found after 60 d. Higher death rates of staphylococci and coliforms were recorded for cheese made in spring than for the cheese made in winter.

Type
Original articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1988

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References

REFERENCES

Association Of Official Analytical Chemists 1984 Official Methods of Analysis, 14th edn.Arlington, VA: AOACGoogle Scholar
Baird-Parker, A. C. 1979 Methods for identifying staphylococci and micrococci. In Identification Methods for Microbiologists pp. 201210 (Eds Skinner, F. A. and Lovelock, D. W.). London: Academic Press. (Society for Applied Bacteriology, Technical Series no. 14)Google Scholar
Bautista, L., Bermejo, M. P. & Nuñez, M. 1986 Seasonal variation and characterization of Micrococcaceae present in ewes' raw milk. Journal of Dairy Research 53 15CrossRefGoogle ScholarPubMed
De Man, J. C., Rogosa, M. & Sharpe, M. E. 1960 A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology 23 130135CrossRefGoogle Scholar
Fernandez Del Pozo, B., Gaya, P., Medina, M., Rodríguez-Marín, M. A. & Nuñez, M. 1988 Changes in chemical and rheological characteristics of La Serena ewes' milk cheese during ripening. Journal of Dairy Research 55 457464CrossRefGoogle Scholar
Gaya, P., Medina, M., Bautista, L. & Nuñez, M. 1984 Survival of Staphylococcus aureus in raw sheep milk Manchego cheeses ripened at different temperatures. Journal of Applied Bacteriology 57 xviiGoogle Scholar
Gaya, P., Medina, M. & Nuñez, M. 1983 Accelerated decrease of Enterobacteriaceae counts during ripening of raw milk Manchego cheese by lactic culture inoculation. Journal of Food Protection 46 305308CrossRefGoogle ScholarPubMed
Gaya, P., Medina, M. & Nuñez, M. 1987 Enterobacteriaceae, coliforms, faecal coliforms and salmonellas in raw ewes' milk. Journal of Applied Bacteriology 62 321326CrossRefGoogle ScholarPubMed
Law, B. A., Sharpe, M. E., Mabbitt, L. A. & Cole, C. B. 1973 Microflora of Cheddar cheese and some of the metabolic products. In Sampling – Microbiological Monitoring of Environments pp. 19 (Eds Skinner, F. A. and Lovelock, D. W.). London: Academic Press. (Society for Applied Bacteriology, Technical Series no. 7)Google Scholar
Martinez Manso, P. & Fernández-Salguero, J. 1978 [Study of some microbial groups in La Serena cheese.] Archivos de Zootecnia 27 9397Google Scholar
Nuñez, J. A., Chavarri, F. J. & Nuñez, M. 1984 Psychrotrophic bacterial flora of raw ewes' milk, with particular reference to Gram-negative rods. Journal of Applied Bacteriology 57 2329CrossRefGoogle ScholarPubMed
Nuñez, M. 1978 Microflora of Cabrales cheese: changes during maturation. Journal of Dairy Research 45 501508CrossRefGoogle Scholar
Nuñez, M., Gaya, P. & Medina, M. 1985 Influence of manufacturing and ripening conditions on the survival of Enterobacteriaceae in Manchego cheese. Journal of Dairy Science 68 794800Google Scholar
Nuñez, M. & Martinez-Moreno, J. L. 1976 [Microbial flora of Manchego cheese. 1. Behaviour of the microbial flora in farm-produced Manchego cheese.] Anales del Instituto Nacional de Investigaciones Agrarias Serie General 4 1131Google Scholar
Ordoñez, J. A., Masso, J. A., Marmol, M. P. & Ramos, M. 1980 [Contribution to the study of Roncal cheese.] Lait 60 283294Google Scholar
Perry, K. D. & Sharpe, M. E. 1960 Lactobacilli in raw milk and in Cheddar cheese. Journal of Dairy Research 27 267275CrossRefGoogle Scholar
Sharpe, M. E. 1979 Identification of the lactic acid bacteria. In Identification Methods for Microbiologists, 2nd edn pp 233259 (Eds Skinner, F. A. and Lovelock, D. W.). London: Academic Press. (Society for Applied Bacteriology, Technical Series no. 14)Google Scholar
Tatini, S. R., Jezeski, J. J., Morris, H. A., Olson, J. C. & Casman, E. P. 1971 Production of staphylococcal enterotoxin A in Cheddar and Colby cheeses. Journal of Dairy Science 54 815825CrossRefGoogle Scholar
Vieira De Sá, F. & Barbosa, M. 1972 Cheese-making with a vegetable rennet from Cardo (Cynara cardunculus). Journal of Dairy Research 39 335343CrossRefGoogle Scholar