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Antioxidant and cholesterol assimilation activities of selected lactobacilli and lactococci cultures

Published online by Cambridge University Press:  29 July 2009

Shalini Jain ,
Hariom Yadav  and
Pushpalata Ravindra Sinha
Shalini Jain
Affiliation:
Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, India
Hariom Yadav*
Affiliation:
Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, India
Pushpalata Ravindra Sinha
Affiliation:
Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, India
*
*For correspondence; e-mail: [email protected]
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Abstract

In present study, three strains of lactic acid bacteria (LAB) viz. Lactobacillus casei, Lactobacillus acidophilus and Lactococcus lactis and milk fermented with these strains have been studied for antioxidant and cholesterol assimilation activities in-vitro and in-vivo, in addition to the effect on total lactobacilli, lactococci and coliform counts into the gut of mice fed with diets supplemented by fermented milk. All three selected strains exhibited potent 2,2-diphenyl-1-picrylhydrazyl, malonaldialdehyde and hydrogen peroxide radical scavenging abilities as well as inhibition of linoleic acid peroxidation activity. These activities were highest in Lb. casei as followed by Lb. acidophilus and Lc. lactis. In addition, these bacterial cultures also exhibited good in-vitro cholesterol assimilation potential. Oral administration to mice of milk fermented with selected LAB strains, slightly decreased blood cholesterol, increased colonization of total lactobacilli and lactococci, and decreased coliforms in the intestinal tissues as well as faecal samples. These results indicate that, selected LAB strains have good antioxidant, hypocholesterolemic and coliform removal activities. It may suggest that, a novel functional food can be obtained by supplementation of selected LAB in milk, which may have various health beneficial properties such as antioxidant and hypocholesterolemic activities.

Keywords

Lactic acid bacteriaLactobacillusAntioxidant activityCholesterol assimilation
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 4 , November 2009 , pp. 385 - 391
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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References

Association of Official Analytical Chemists 1995 In: Official methods of analysis, (16th ed.). Garthersburg: AOAC InternatonalGoogle Scholar
Aubourg, SP 1993 Review: interaction of malondialdehyde with biological molecules—new trend about reactivity and significance. International Journal of Food Science and Technology 28 323–335Google Scholar
Baker, MA & He, SQ 1991 Elaboration of cellular DNA breaks by hydroperoxides. Free Radical Biology and Medicine 11 563–572CrossRefGoogle ScholarPubMed
Dave, RI & Shah, NP 1996 Evaluation of media for selective enumeration of Streptococcus thermophilus, Lactobacillus delbrueckii ssp.bulgaricus, Lactobacillus acidophilus and Bifidobacteria. Journal of Dairy Science 79 1529–1536Google Scholar
Decker, E & Faraji, H 1990 Inhibition of lipid peroxidation by carnosine. Journal of AOCS 67 650–652Google Scholar
DeCoster, VA 2008 Diabetes treatments. Journal of Gerontology and Social Work 50 105–129CrossRefGoogle ScholarPubMed
Fujiwara, D, Inoue, S, Wakabayashi, H & Fujii, T 2004 The anti-allergic effects of lactic acid bacteria are strain dependent and mediated by effects on both Th1/Th2 cytokine expression and balance. International Archives of Allergy and Immunology 135 205–215Google Scholar
Gilliland, SE & Walker, DK 1990 Factors to consider when selecting a culture of Lactobacillus acidophilus as a dietary adjunct to produce a hypocholesterolemic effect in humans. Journal of Dairy Science 73 905–911Google Scholar
Gilliland, SE, Nelson, CR & Maxwell, C 1985 Assimilation of cholesterol by Lactobacillus acidophilus. Applied and Environmental Microbiology 49 377–381Google Scholar
Gorbach, SL 1990 Lactic acid bacteria and human health. Annals of Medicine 22 37–41CrossRefGoogle ScholarPubMed
Grajek, W, Olejnik, A & Sip, A 2005 Probiotics, prebiotics and antioxidants as functional foods. Acta Biochemica Polonica 52 665–671Google Scholar
Halliwell, B & Chirico, S 1993 Lipid peroxidation, its mechanism, measurement and significance. American Journal of Clinical Nutrition 57 715S–725SCrossRefGoogle ScholarPubMed
Hosono, A & Tono-oka, T 1995 Binding of cholesterol with lactic acid bacteria cells. Milchwissenschaft 50 556–560Google Scholar
Kaizu, H, Sasaki, M, Nakajima, H & Suzuki, Y 1993 Effect of antioxidative lactic acid bacteria on rats fed a diet deficient in vitamin E. Journal of Dairy Science 76 2493–2499Google Scholar
Kimoto, H, Ohmomo, S & Okamoto, T 2002 Cholesterol removal from media by lactococci. Journal of Dairy Science 85 3182–3188CrossRefGoogle ScholarPubMed
Klaenhammer, TR 1982 Microbiological considerations in selection and preparation of Lactobacillus strains for use as dietary adjuncts. Journal of Dairy Science 65 1339–1341CrossRefGoogle Scholar
Lidbeck, A, Gustafasson, JA & Nord, C 1987 Impact of Lactobacillus acidophilus supplements on the human oropharyngeal and intestinal micro-flora. Scavandian Journal of Infectious Diseases 19 531–537Google Scholar
Lin, MY & Chang, FJ 2000 Antioxidative effect of intestinal bacteria Bifidobacterium longum ATCC 15708 and Lactobacillus acidophilus ATCC 4356. Digestive Diseases Science 45 1617–1622Google Scholar
Lin, MY & Yen, CL 1999 Reactive oxygen species and lipid peroxidation product-scavenging ability of yogurt organisms. Journal of Dairy Science 82 1629–1634Google Scholar
Liong, MT & Shah, NP 2005 Acid and bile tolerance and cholesterol removal ability of lactobacilli strains. Journal of Dairy Science 88 55–66CrossRefGoogle ScholarPubMed
Maramaldi, P, Dungan, S & Poorvu, NL 2008 Cancer treatments. Journal of Gerontology and Social Work 50 45–77Google Scholar
Ohkawa, H, Ohishi, N & Yagi, K 1979 Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 95 351–358Google Scholar
Ouwehand, AC, Kirjavainien, PV, Shortt, C & Salminen, S 1999 Probiotics: mechanisms and established effects. International Dairy Journal 9 43–52Google Scholar
Pham, M, Lemberg, DA & Day, AS 2008 Probiotics: sorting the evidence from the myths. The Medical Journal of Australia 188 304–308Google Scholar
Ravula, RR & Shah, NP 1998 Selective enumeration of Lactobacillus casei from yogurt and fermented milk drinks. Biotechnology Techniques 12 819–822Google Scholar
Roessner, A, Kuester, D, Malfertheiner, P & Schneider-Stock, R 2008 Oxidative stress in ulcerative colitis-associated carcinogenesis. Pathology Research and Practice 204 511–524Google Scholar
Rudel, LL & Moris, MD 1973 Determination of cholesterol using o-phthalaldehyde. Journal of Lipid Research 14 354–366Google Scholar
Sanford, PA 1992 Digestive system physiology. London: Edward ArnoldGoogle Scholar
Saxelin, M, Pessi, T & Salminen, S 1995 Faecal recovery following oral administration of Lactobacillus strain GG (ATCC 53103) in gelatine capsules to healthy volunteers. International Journal of Food Microbiology 25 199–203Google Scholar
Shibata, S, Natori, Y, Nishihara, T, Tomisaka, K, Matsumoto, K, Sansawa, H & Nguyen, C 2003 Antioxidant and anticataract effect of Chlorella on rats with streptozotocin-induced diabetes. Journal of Nutritional Science and Vitaminology 49 334–339Google Scholar
Sinclair, AJ, Barnett, AH & Lunec, J 1991 Free radicals and antioxidant systems in health and disease. Journal of Applied Medicine 21 409–417Google Scholar
Sonnenburg, JL, Chen, CT & Gordon, JI 2006 Genomic and metabolic studies of the impact of probiotics on a model gut symbiont and host. PLoS Biology 4 e413Google Scholar
Taranto, MP, Fernandez, ML, Murga, LG & deValdez, GF 2003 Bile salts and cholesterol induce changes in the lipid cell membrane of Lactobacillus reuteri. Journal of Applied and Microbiology 95 86–91Google Scholar
Terzaghi, BE & Sandine, W 1975 Improved medium for lactic acid streptococci and their bacteriophages. Applied Microbiology 29 807–813Google Scholar
Terzaghi, BE & Sandine, W 1975 Improved medium for lactic acid streptococci and their bacteriophages. Applied Microbiology 29 807–813Google Scholar
Wadstrom, T 1998 Adherence traits and mechanisms of microbial adhesion in the gut. Boiller's Clinical and Tropical Medical Common Disease 3 417–433Google Scholar
Willcox, BJ, Curb, JD & Rodriguez, BL 2008 Antioxidants in cardiovascular health and disease: key lessons from epidemiologic studies. American Journal of Cardiology 101 75D–86DCrossRefGoogle ScholarPubMed
Wolfe, WC 1962 Spectrophotometric determination of hydroperoxide in diethyl ether. Analytical Chemistry 34 1328–1330Google Scholar
Yadav, H, Jain, S & Sinha, PR 2006 Effect of skim milk and dahi (yogurt) on blood glucose, insulin and lipid profile in rats fed with high fructose diet. Journal of Medicinal Food 9 328–335Google Scholar
Yadav, H, Jain, S & Sinha, PR 2007 Antidiabetic effect of probiotic dahi containing Lactobacillus acidophilus, Lactobacillus casei and Lactococcus lactis bacteria in high fructose diet fed rats. Nutrition 72 62–68Google Scholar
Yadav, H, Jain, S & Sinha, PR 2008 Oral administration of dahi containing probiotic Lactobacillus acidophilus and Lactobacillus casei ameliorated the Streptozotocin-induced oxidative stress and dyslipidemia in rats. Journal of Dairy Research 75 189–195Google Scholar
Zisman, TL & Rubin, DT 2008 Colorectal cancer and dysplasia in inflammatory bowel disease. World Journal of Gastroenterology 14 2662–2669CrossRefGoogle ScholarPubMed
Zommara, M, Takagi, H, Sakono, M, Suzuki, Y & Imaizami, K 1994 Effect of milk whey and its fermentation products by lactic acid bacteria on mitochondrial lipid peroxide and hepatic injury in bile duct-ligated rats. Bioscience Biotechnology and Biochemistry 58 1213–1217CrossRefGoogle Scholar
Zommara, M, Toubo, H, Sakono, M & Imaizumi, K 1998 Prevention of peroxidative stress in rats fed on a low vitamin E containing diet by supplementing with fermented bovine milk whey preparation: Effect of lactic acid bacteria and β-lactoglobulin on antiperoxidative action. Bioscience Biotechnology and Biochemistry 62 710–717Google Scholar