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An automated enzymic micromethod for the measurement of fat in human milk

Published online by Cambridge University Press:  01 June 2009

Alan Lucas ,
Geoffrey J. Hudson ,
Peter Simpson ,
Timothy J. Cole  and
Brian A. Baker
Alan Lucas
Affiliation:
Medical Research Council Dunn Nutrition Unit, Cambridge CB4 1XJ, UK
Geoffrey J. Hudson
Affiliation:
Medical Research Council Dunn Nutrition Unit, Cambridge CB4 1XJ, UK
Peter Simpson
Affiliation:
Medical Research Council Dunn Nutrition Unit, Cambridge CB4 1XJ, UK
Timothy J. Cole
Affiliation:
Medical Research Council Dunn Nutrition Unit, Cambridge CB4 1XJ, UK
Brian A. Baker
Affiliation:
Medical Research Council Dunn Nutrition Unit, Cambridge CB4 1XJ, UK
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Summary

Most (98%) of the fat in human milk is present as triglycerides. This paper describes the use of a clarification procedure that enables the level of human milk fat to be determined by measurement of glycerol released by enzymic hydrolysis of triglycerides. The method requires only 10–50 μl milk, thus presenting a possible technique for work with small mammals, and is suitable for use with autoanalysers, permitting rapid sample throughput.

Type
Original Articles
Information
Journal of Dairy Research , Volume 54 , Issue 4 , November 1987 , pp. 487 - 492
Copyright
Copyright © Proprietors of Journal of Dairy Research 1987

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References

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