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Allelic variations in coding regions of the vitamin D receptor gene in dairy cows and potential susceptibility to periparturient hypocalcaemia

Published online by Cambridge University Press:  11 September 2012

Carolin Deiner ,
Maria Reiche ,
Dirk Lassner ,
Desirée Grienitz ,
Sven Twardziok ,
Anne Moesch ,
Peter Wenning  and
Holger Martens
Carolin Deiner*
Affiliation:
Institute of Veterinary Physiology, Faculty of Veterinary Medicine, FU Berlin, Berlin, Germany
Maria Reiche
Affiliation:
Institute of Veterinary Physiology, Faculty of Veterinary Medicine, FU Berlin, Berlin, Germany
Dirk Lassner
Affiliation:
IKDT, Berlin, Germany
Desirée Grienitz
Affiliation:
IKDT, Berlin, Germany
Sven Twardziok
Affiliation:
Institute of Molecular Biology and Bioinformatics, Charité Universitätsmedizin Berlin, Berlin, Germany
Anne Moesch
Affiliation:
Institute of Veterinary Physiology, Faculty of Veterinary Medicine, FU Berlin, Berlin, Germany
Peter Wenning
Affiliation:
Clinic for Cattle, University of Veterinary Medicine Hannover, Hannover, Germany
Holger Martens
Affiliation:
Institute of Veterinary Physiology, Faculty of Veterinary Medicine, FU Berlin, Berlin, Germany
*
*For correspondence; e-mail: [email protected]
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Abstract

Periparturient hypocalcaemia (milk fever) is a disorder of Ca metabolism in dairy cattle primarily affecting multiparous cows. The major reasons for the rapid decrease of blood Ca concentration after calving are the prompt increase of Ca secretion into the colostrum and the delayed activation of Ca regulation mechanisms including calcitriol, a metabolite of vitamin D. In man, vitamin D receptor (VDR) gene polymorphisms are reported to be associated with disturbances of Ca metabolism, whereas data confirming the same in dairy cows are still missing. Moreover, polymorphisms that only affect non-coding regions are sometimes difficult to ascribe to a specific disorder as pathways and unequivocal links remain elusive. Therefore, the idea of the present study was to investigate in a small group of dairy cows with documented clinical records whether polymorphisms in the coding regions of the VDR gene existed and whether these potentially found variations were correlated with the incidence of periparturient hypocalcaemia. For this purpose, blood DNA was isolated from 26 dairy cows in their 4th to 6th lactation, out of which 17 had experienced hypocalcaemia at least once, whereas 9 cows had never undergone periparturient hypocalcaemia in their lifetime. The 10 VDR exons and small parts of adjacent introns were sequenced and compared with the Bos taurus VDR sequence published on NCBI based on the DNA of one Hereford cow. In total, 8 sequence alterations were detected in the fragments, which were primarily heterozygous. However, only 4 of them were really located on exons thereby potentially causing changes of the encoded amino acid of the VDR protein, but were not correlated with the incidence of periparturient hypocalcaemia. Certainly, this lack of statistical correlation could be due to the small number of animals included; anyhow, it was not encouraging enough to initiate a larger study with hundreds of cows and document blood Ca levels post partum for at least four lactations.

Keywords

Vitamin D receptorpolymorphismdairy cowhypocalcaemia
Type
Research Article
Information
Journal of Dairy Research , Volume 79 , Issue 4 , November 2012 , pp. 423 - 428
Copyright
Copyright © Proprietors of Journal of Dairy Research 2012

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References

Agresti, A 1992 A survey of exact inference for contingency tables. Statistical Science 7 131–153Google Scholar
Arai, H, Miyamoto, KI, Yoshida, M, Yamamoto, H, Taketani, Y, Morita, K, Kubota, M, Yoshida, S, Ikeda, M, Watabe, F, Kanemasa, Y & Takeda, E 2001 The polymorphism in the caudal-related homeodomain protein Cdx-2 binding element in the human vitamin D receptor gene. Journal of Bone and Mineral Research 16 1256–1264CrossRefGoogle ScholarPubMed
Bender, R & Lange, S 2001 Adjusting for multiple testing – when and how? Journal of Clinical Epidemiology 54 343–349Google Scholar
Bezerra, FF, Cabello, GM, Mendonca, LM & Donangelo, CM 2008 Bone mass and breast milk calcium concentration are associated with vitamin D receptor gene polymorphisms in adolescent mothers. Journal of Nutrition 138 277–281CrossRefGoogle ScholarPubMed
Degaris, PJ & Lean, IJ 2008 Milk fever in dairy cows: a review of pathophysiology and control principles. Veterinary Journal 176 58–69Google Scholar
Ebeling, PR, Sandgren, ME, Dimagno, EP, Lane, AW, Deluca, HF & Riggs, BL 1992 Evidence of an age-related decrease in intestinal responsiveness to vitamin D: relationship between serum 1,25-dihydroxyvitamin D3 and intestinal vitamin D receptor concentrations in normal women. Journal of Clinical Endocrinology and Metabolism 75 176–182Google Scholar
Fang, Y, Van Meurs, JB, Bergink, AP, Hofman, A, Van Duijn, CM, Van Leeuwen, JP, Pols, HA & Uitterlinden, AG 2003 Cdx-2 polymorphism in the promoter region of the human vitamin D receptor gene determines susceptibility to fracture in the elderly. Journal of Bone and Mineral Research 18 1632–1641Google Scholar
Fang, Y, Van Meurs, JB, D'alesio, A, Jhamai, M, Zhao, H, Rivadeneira, F, Hofman, A, Van Leeuwen, JP, Jehan, F, Pols, HA & Uitterlinden, AG 2005 Promoter and 3'-untranslated-region haplotypes in the vitamin d receptor gene predispose to osteoporotic fracture: the rotterdam study. American Journal of Human genetics 77 807–823Google Scholar
Freeman, GHH & Halton, JH 1951 Note on exact treatment of contingency, goodness of fit and other problems of significance. Biometrika 38 141–149Google Scholar
Haussler, MR, Haussler, CA, Jurutka, PW, Thompson, PD, Hsieh, JC, Remus, LS, Selznick, SH & Whitfield, GK 1997 The vitamin D hormone and its nuclear receptor: molecular actions and disease states. Journal of Endocrinology 154 (Suppl.) S57–S73Google Scholar
Hoenderop, JG, Nilius, B & Bindels, RJ 2005 Calcium absorption across epithelia. Physiological Reviews 85 373–422CrossRefGoogle ScholarPubMed
Holick, MF 1995 Noncalcemic actions of 1,25-dihydroxyvitamin D3 and clinical applications. Bone 17(2 Suppl.) 107S–111SGoogle Scholar
Horst, RL, Goff, JP & Reinhardt, TA 1990 Advancing age results in reduction of intestinal and bone 1,25-dihydroxyvitamin D receptor. Endocrinology 126 1053–1057Google Scholar
Horst, RL, Goff, JP & Reinhardt, TA 1994 Calcium and vitamin D metabolism in the dairy cow. Journal of Dairy Science 77 1936–1951Google Scholar
Kinyamu, HK, Gallagher, JC, Prahl, JM, Deluca, HF, Petranick, KM & Lanspa, SJ 1997 Association between intestinal vitamin D receptor, calcium absorption, and serum 1,25 dihydroxyvitamin D in normal young and elderly women. Journal of Bone and Mineral Research 12 922–928CrossRefGoogle Scholar
Lee, GS, Choi, KC, Park, SM, An, BS, Cho, MC & Jeung, EB 2003 Expression of human Calbindin-D(9k) correlated with age, vitamin D receptor and blood calcium level in the gastrointestinal tissues. Clinical Biochemistry 36 255–261.Google Scholar
Liang, CT, Barnes, J, Imanaka, S & Deluca, HF 1994 Alterations in mRNA expression of duodenal 1,25-dihydroxyvitamin D3 receptor and vitamin D-dependent calcium binding protein in aged Wistar rats. Experimental Gerontology 29 179–186Google Scholar
Liesegang, A, Riner, K & Boos, A 2007 Effects of gestation and lactation on vitamin D receptor amounts in goats and sheep. Domestic Animal Endocrinology 33 190–202CrossRefGoogle ScholarPubMed
Liesegang, A, Singer, K & Boos, A 2008. Vitamin D receptor amounts across different segments of the gastrointestinal tract in Brown Swiss and Holstein Frisean cows of different age. Journal of Animal Physiology and Animal Nutrition (Berlin) 92 316–323Google Scholar
Malloy, PJ, Eccleshall, TR, Gross, C, Van Maldergem, L, Bouillon, R & Feldman, D 1997 Hereditary vitamin D resistant rickets caused by a novel mutation in the vitamin D receptor that results in decreased affinity for hormone and cellular hyporesponsiveness. Journal of Clinical Investigation 99 297–304CrossRefGoogle ScholarPubMed
Merke, J, Milde, P, Lewicka, S, Hugel, U, Klaus, G, Mangelsdorf, DJ, Haussler, MR, Rauterberg, EW & Ritz, E 1989 Identification and regulation of 1,25-dihydroxyvitamin D3 receptor activity and biosynthesis of 1,25-dihydroxyvitamin D3. Studies in cultured bovine aortic endothelial cells and human dermal capillaries. Journal of Clinical Investigation 83 1903–1915Google Scholar
NCBI. 2011 dbSNP-Short genetic variations. http://www.ncbi.nlm.nih.gov/sites/entrez?db=snp&cmd=search&term=VDR+bovine. Accessed 18 September 2011Google Scholar
Uitterlinden, AG, Fang, Y, Bergink, AP, Van Meurs, JB, Van Leeuwen, HP & Pols, HA 2002 The role of vitamin D receptor gene polymorphisms in bone biology. Molecular and Cellular Endocrinology 197 15–21Google Scholar
Uitterlinden, AG, Fang, Y, Van Meurs, JB, Van Leeuwen, H & Pols, HA 2004 Vitamin D receptor gene polymorphisms in relation to Vitamin D related disease states. Journal of Steroid Biochemistry and Molecular Biology 89–90, 187–193Google Scholar
Uitterlinden, AG & Ralston, SH 2006. The association between common vitamin D receptor gene variations and osteoporosis: a participant-level meta-analysis. Annals of Internal Medicine 145 255–264Google Scholar
Vanraden, PM, Van Tassell, CP, Wiggans, GR, Sonstegard, TS, Schnabel, RD, Taylor, JF & Schenkel, FS 2009 Invited review: Reliability of genomic predictions for North American Holstein bulls. Journal of Dairy Science 92 16–24CrossRefGoogle ScholarPubMed
Whitfield, GK, Remus, LS, Jurutka, PW, Zitzer, H, Oza, AK, Dang, HT, Haussler, CA, Galligan, MA, Thatcher, ML, Encinas Dominguez, C & Haussler, MR 2001 Functionally relevant polymorphisms in the human nuclear vitamin D receptor gene. Molecular and Cellular Endocrinology 177 145–159Google Scholar
Wood, RJ & Fleet, JC 1998 The genetics of osteoporosis: vitamin D receptor polymorphisms. Annual Review of Nutrition 18 233–258CrossRefGoogle ScholarPubMed
Yamagashi, NMM & Naito, Y 2006 The expression of genes for transepithelial calcium-transporting proteins in the bovine duodenum. Veterinary Journal 171 363–366Google Scholar