Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-26T11:58:34.389Z Has data issue: false hasContentIssue false
  • English
  • Français

A 43Ca and 31P NMR study of the calcium and phosphate equilibria in heated milk solutions

Published online by Cambridge University Press:  01 June 2009

N. Magnus Wahlgren ,
Petr Dejmek  and
Torbjörn Drakenberg
N. Magnus Wahlgren
Affiliation:
Department of Food Technology and University of Lund, Box 124, S-221 00 Lund, Sweden
Petr Dejmek
Affiliation:
Department of Food Technology and University of Lund, Box 124, S-221 00 Lund, Sweden
Torbjörn Drakenberg
Affiliation:
Department of Physical Chemistry 2, Chemical Centre, University of Lund, Box 124, S-221 00 Lund, Sweden
Get access Rights & Permissions [Opens in a new window]

Summary

Casein micelle suspensions, colloidal Ca phosphate-free casein solution and simulated milk ultrafiltrate (SMUF) were studied using high-resolution 43Ca and 31P NMR in the temperature range 4–64 °C. Only one 43Ca and one 31P signal was obtained for each solution. Signal intensities and line widths varied with the environment and were affected by the redistribution of Ca and P during heating and cooling. Based upon the temperature-dependent broadening of the 43Ca signal, five different Ca environments were discerned in the heated milk fractions. The Ca state, induced by heating of a casein-containing milk salt solution, differed from both the state of Ca present in micellar colloidal Ca phosphate and from the state of Ca induced by the heating of SMUF.

Type
Original Articles
Information
Journal of Dairy Research , Volume 57 , Issue 3 , August 1990 , pp. 355 - 364
Copyright
Copyright © Proprietors of Journal of Dairy Research 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Brulé, G., Real Del Sole, E., Fauquant, J. & Fiaud, G. 1978 Mineral salts stability in aqueous phase of milk: influence of heat treatments. Journal of Dairy Science 61 1225–1232CrossRefGoogle Scholar
Brulé, G. & Fauquant, J. 1981 Mineral balance in skim-milk and milk retentate: effect of physicochemical characteristics of the aqueous phase. Journal of Dairy Research 48 91–97CrossRefGoogle Scholar
Dalgleish, D. G. 1973 A study of the interaction of calcium ions with bovine αs1-casein using fluorescence spectroscopy. European Journal of Biochemistry 40 375–380CrossRefGoogle Scholar
Davies, D. T. & White, J. C. D. 1960 The use of ultrafiltration and dialysis in isolating the aqueous phase of milk and in determining the partition of milk constituents between the aqueous and disperse phases. Journal of Dairy Research 27 171–190CrossRefGoogle Scholar
Dickson, I. R. & Perkins, D. J. 1971 Studies on the interactions between purified bovine caseins and alkaline-earth-metal ions. Biochemical Journal 124 235–240CrossRefGoogle ScholarPubMed
Drakenberg, T. & Forsén, S. 1982 The alkaline earth-metals-biological applications. In The Multinuclear Approach to NMR Spectroscopy, pp. 309–328 (Eds Lambert, J. B. and Riddell, F. G.). Dordrecht: D. Reidel.Google Scholar
Drakenberg, T., Forsén, S. & Lilja, H. 1983 43Ca NMR studies of calcium binding to proteins: interpretation of experimental data by bandshape analysis. Journal of Magnetic Resonance 53 412–22Google Scholar
Glasstone, S., Laidler, K. J. & Eyring, H. 1941 The Theory of Rate Processes, pp. 153–201New York: McGraw HillGoogle Scholar
Harris, H. K. 1986 In Nuclear Magnetic Resonance Spectroscopy, pp. 9–11Avon: Longman Scientific & TechnicalGoogle Scholar
Holt, C. 1985 The milk salts: their secretion, concentrations and physical chemistry. In Developments in Dairy Chemistry-3, Lactose and minor constituents pp. 143–181 (Ed. Fox, P. F.). London: Elsevier Applied Science PublishersCrossRefGoogle Scholar
Holt, C., Parker, T. G. & Dalgleish, D. G. 1975 The thermochemistry of reactions between αs1-casein and calcium chloride. Biochimica et Biophysica Acta 379 638–644CrossRefGoogle Scholar
Holt, C., Dalgleish, D. G. & Jenness, R. 1981 Calculation of the ion equilibria in milk diffusate and comparison with experiment. Analytical Biochemistry 113 154–163CrossRefGoogle ScholarPubMed
Imade, T., Sato, Y. & Noguchi, H. 1977 Interaction of calcium ion with bovine caseins. Agricultural and Biological Chemistry 41 2131–2137Google Scholar
Jenness, R. & Koops, J. 1962 Preparation and properties of a salt solution which simulates milk ultrafiltrate. Netherlands Milk and Dairy Journal 16 153–164Google Scholar
Lyster, R. L. J. 1979 The equilibria of calcium and phosphate ions with the micellar calcium phosphate in cow's milk. Journal of Dairy Research 46 343–346CrossRefGoogle ScholarPubMed
Ono, T., Kaminogawa, S., Odagiri, S. & Yamauchi, K. 1976 A study on the binding of calcium ions to αs1- casein. Agricultural and Biological Chemistry 40 1717–1723Google Scholar
Parker, T. G. & Dalgleish, D. G. 1981 Binding of calcium ions to bovine β-casein. Journal of Dairy Research 48 71–76CrossRefGoogle ScholarPubMed
Pierre, A., Brulé, G. & Fauquant, J. 1983 [Study of Ca exchange in milk using 45Ca.] Lait 63 473–489CrossRefGoogle Scholar
Pouliot, Y., Boulet, M. & Paquin, P. 1989 a Observations on the heat-induced salt balance changes in milk. I. Effect of heating time between 4 and 90 °C. Journal of Dairy Research 56 185–192CrossRefGoogle Scholar
Pouliot, Y., Boulet, M. & Paquin, P. 1989 b Observations on the heat-induced salt balance changes in milk. II. Reversibility on cooling. Journal of Dairy Research 56 193–199CrossRefGoogle Scholar
Pyne, G. T. & McGann, T. C. A. 1960 The colloidal phosphate of milk II. Influence of citrate. Journal of Dairy Research 27 9–17CrossRefGoogle Scholar
Schmidt, D. G. 1982 Association of caseins and casein micelle structure. In Developments in Dairy Chemistry-1. Proteins. pp. 61–86 (Ed. Fox, P. F.). London: ElsevierGoogle Scholar
Schmidt, D. G., Both, P., Visser, S., Slangen, K. J. & Van Rooijen, P. J. 1987 Studies on the precipitation of calcium phosphate. II. Experiments in the pH range 7·3 to 5·8 at 25 and 50 °C in the presence of additives. Netherlands Milk and Dairy Journal 41 121–136Google Scholar
Slattery, C. W. 1975 Cation binding to αs1-casein B. A comparison of electrostatic models. Biophysical Chemistry 3 83–89CrossRefGoogle Scholar
Tsai, M.-D., Drakenberg, T., Thulin, E. & Forsén, S. 1987 Is the binding of magnesium(II) to calmodulin significant ? An investigation by magnesium-25 nuclear magnetic resonance. Biochemistry 26 3635–3643CrossRefGoogle ScholarPubMed
Visser, J., Minihan, A., Smits, P., Tjan, S. B. & Heertje, I. 1986 Effects of pH and temperature on the milk salt System. Netherlands Milk and Dairy Journal 40 351–368Google Scholar
Vogel, H. J. & Forsén, S. 1986 NMR studies of calcium-binding proteins. Biological Magnetic Resonance 7 249–309Google Scholar
Waugh, D. F., Slattery, C. W. & Creamer, L. K. 1971 Binding of cations to caseins. Site binding, Donnan binding and System characteristics. Biochemistry 10 817–823CrossRefGoogle ScholarPubMed
Wiechen, A. & Knoop, A. M. 1978 [Investigations on Ca distribution between serum and casein by means of 45Ca in cooled and pasteurized milk]. Milchwissenschaft 33 213–215Google Scholar
Yamauchi, K., Yoneda, Y., Koga, Y. & Tsugo, T. 1969 Exchangeability of colloidal calcium in milk with soluble calcium. Agricultural and Biological Chemistry 33 907–914CrossRefGoogle Scholar
Yamauchi, K. & Yoneda, Y. 1977 Effect of some treatments of milk on the exchangeability of colloidal calcium in milk with soluble calcium. Agricultural and Biological Chemistry 41 2395–2399Google Scholar