Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-05T14:29:57.432Z Has data issue: false hasContentIssue false

262. The microbiology of silage made by the addition of mineral acids to crops rich in protein

Published online by Cambridge University Press:  01 June 2009

A. Cunningham
Affiliation:
From The College of Agriculture, Edinburgh
A. M. Smith
Affiliation:
From The College of Agriculture, Edinburgh

Extract

1. The microflora of A.I.V. silage consists mainly of lactic acid bacteria—lactobacilli, streptococci, micrococci and sarcinae. Some samples also contain yeasts and yeast-like organisms.

2. Among the lactobacilli, both homo- and heterofermentative types are represented. The former include strains of Lactobacillus plantarum (Orla- Jensen) Bergey et. al.; in the heterofermentative group L. brevis (Orla-Jensen) Bergey et al. has been found.

3. Cultures of a motile homofermentative lactobacillus were isolated; this appears to be a new type. Among the unidentified heterofermentative lactobacilli, forms which may prove to be similar to the inactive group of Pederson were encountered.

4. The homofermentative streptococci were found to belong to the Streptococcus lactis group; the heterofermentative forms were identified with Leuconostoc mesenteroides (Cienkowski) van Tieghem.

5. Strains of unidentified homofermentative micrococci and sarcinae were isolated and shown to possess characteristics not usually associated with organisms of these types.

6. Streptococci, micrococci and motile lactobacilli were found mainly in fodder recently ensiled; the majority of the lactobacilli and sarcinae were associated with the older samples.

7. The characteristics which proved to be most valuable for the differentiations of the organisms were ability to produce carbon dioxide, percentage of lactic acid formed and lactic-acetic acid ratio.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1940

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

(1) Allen, L. A. & Harrison, J. (1936). Ann. appl. Biol. 23, 546–57.Google Scholar
(2) Clark, E. P. & Hillig, F. (1938). J. Ass. off. agric. Chem., Wash., 21, 684–8.Google Scholar
(3) Cunningham, A. (1934). Practical Bacteriology. Edinburgh: Oliver and Boyd.Google Scholar
(4) Cunningham, A. & Smith, A. M. (1939). Zbl. Bakt. II, 100, 394408.Google Scholar
(5) Demeter, K. J. (1930). Zbl. Bakt. 82, 7199.Google Scholar
(6) Dyer, D. C. (1917). J. biol. Chem. 28, 445–73.Google Scholar
(7) Fred, E. B., Peterson, W. H. & Anderson, J. A.Google Scholar
(a) (1921) J. biol. Chem. 48; 385412;Google Scholar
(b) (1922) J. biol. Chem. 53, 111–23.Google Scholar
(8) Fred, E. B., Peterson, W. H. & Davenport, A. (1919). J. biol. Chem. 39, 347–84.Google Scholar
(9) Fred, E. B., Peterson, W. H. & Davenport, A. (1920). J. biol. Chem. 42, 175–89.Google Scholar
(10) Gray, P. H. H. (1926). J. Bact. 12, 273–4.Google Scholar
(11) Hucker, G. J. (1928). Tech. Bull. N.Y. St. agric. Exp. Sta. no. 144.Google Scholar
(12) Hucker, G. J. & Pederson, C. S. (1931). Zbl. Bakt. II, 85, 65114.Google Scholar
(13) Karstrom, H. & Virtanen, A. I. (1937). Ann. Acad. Sci. fenn., Ser. A, 48, 28.Google Scholar
(14) Kopeloff, N. & Cohen, P. (1928). Stain Tech. 3, 6470.Google Scholar
(15) Orla-Jensen, S. (1904). Zbl. Bakt. II, 13, 435.Google Scholar
(16) Orla-Jensen, S. (1919). The Lactic Acid Bacteria. Kobenhavn.Google Scholar
(17) Orla-Jensen, A. D. (1933). J. Soc. chem. Ind. 52, 374–9 T.Google Scholar
(18) Pederson, C. S. (1936). J. Bad. 31, 217–24.Google Scholar
(19) Pederson, C. S. (1938). J. Bact. 35, 95108.Google Scholar
(20) Sherman, J. M. (1937). Bact. Rev. 1, 197.Google Scholar
(21) Smith, A. M. (1938). Analyst, 63, 777–81.CrossRefGoogle Scholar
(22) Virtanen, A. I. & Pulkki, L. (1928). J. Amer. chem. Soc. 50, 3138–51.CrossRefGoogle Scholar
(23) Virtanen, A. I. (1937). Zbl. Bakt. II, 95, 472–7.Google Scholar
(24) Virtanen, A. I. (1938). Cattle Fodder and Human Nutrition, pp. 78102. Camb. Univ. Press.Google Scholar
(25) Weinstein, L. & Rettoer, L. F. (1932). J. Bact. 24, 128.Google Scholar
(26) Wiegner, G. (1926). Anleit. z. quantitat. agrikulturchem. Prakt. Berlin.Google Scholar