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Ability of bovine mammary macrophages to enhance proliferation of autologous blood and mammary secretion lymphocytes

Published online by Cambridge University Press:  01 June 2009

Carlos Concha  and
Olof Holmbebg
Carlos Concha
Affiliation:
National Veterinary Institute and Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Box 7073, S-750 07 Uppsala, Sweden
Olof Holmbebg
Affiliation:
National Veterinary Institute and Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Box 7073, S-750 07 Uppsala, Sweden
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Summary

Cells were obtained by centrifuging the mammary secretion of healthy udders of 19 cows during the dry-period and during mid-lactation. The suspended cells were incubated in plastic wells. Those adhered cells classified as mammary macrophages were incubated with pokeweed mitogen (PWM). Autologous peripheral blood lymphocytes were added to wells containing untreated macrophage cultures or cultures pretreated with PWM. In seven cows autologous dry-period mammary lymphocytes were added instead of blood lymphocytes. The macrophages + lymphocyte cultures were subjected to the lymphocyte stimulation test (LST). For comparison, peripheral blood lymphocytes and dry-period secretion lymphocytes were also subjected to the LST in the presence of PWM. In all cases, mitogenic responses were higher in pretreated macrophage cultures than in background control cultures.

The stimulation indices (SI) showed that PWM-pretreated dry-period mammary macrophages enhanced the proliferation of autologous peripheral blood lymphocytes to a greater extent than did blood lymphoeytes plus PWM (49±10 v. 30 ± 6; P ≤ 0·05). Mammary macrophages taken from the same cows but during midlactation also clearly induced proliferation of autologous peripheral blood lymphocytes but to a lesser extent than dry-period macrophages (16 ± 2 v. 49±10; 16±2 v. 30±6; P ≤ 0·01 and P ≤ 0·05).

The PWM pretreatment of mammary macrophages increascd the proliferation of autologous dry-period mammary lymphocytes by at least a factor of three (28±8 v. 8±2 P ≤ O·05).

The present results indicate that bovine mammary macrophages pretreated with PWM enhance proliferation as well as modulation of mammary and peripheral blood lymphocytes. The modulation of lymphocyte stimulation as demonstrated here in vitro, has great significance regarding aspects of local immunostimulation related to modern treatment of mastitis.

Type
Original Articles
Information
Journal of Dairy Research , Volume 57 , Issue 1 , February 1990 , pp. 7 - 16
Copyright
Copyright © Proprietors of Journal of Dairy Research 1990

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References

REFERENCES

Bielefeldt Ohmann, H., Filion, L. G. & Babiuk, L. A. 1984 Cellular interactions in the generation of bovine Cou A-suppressor cells and in the mitogenic proliferative response. Veterinary Immunology and Immunopathology 6 320–307Google Scholar
Barta, O., Shaffer, L. M. & Huang, L. J. 1984 Separations of lymphocytes, monocytes and neutrophils. In Laboratory Techniques of Veterinary Clinical Immunology pp. 31–42 (Ed. Barta, O.) Springfield Illinois: Charles C. ThomasGoogle Scholar
Collins, R. A. & Oldham, G. 1986 Proliferative responses and IL-2 production by mononuclear cells from bovine mammary secretions, and the effect of mammary secretions on peripheral blood lymphocytes. Immunology 58 647–651Google ScholarPubMed
Concha, C., Holmberg, O. & Morein, B. 1980 Characterization and response to mitogens of mammary lymphocytes from the bovine dry-period secretion. Journal of Dairy Research 47 305–311CrossRefGoogle ScholarPubMed
Craven, N. & Williams, M. R. 1985 Defences of the bovine mammary gland against infection and prospects for their enhancement. Veterinary Immunology and Immunopathology 10 71–127CrossRefGoogle ScholarPubMed
Diaz-Jouanen, E. & Williams, R. C. 1974 T and B lymphocytes in human colostrum. Clinical Immunology and Immunopathology 3 248–255CrossRefGoogle Scholar
Enright, F. M. & Jeffers, G. W. 1984 Function tests for mononuclear phagocytes. In Laboratory Techniques of Veterinary Clinical Immunology pp. 58–64 (Ed. Barta, O.) Springfield Illinois: Charles C. ThomasGoogle Scholar
Fenichel, R. L. & Chirigos, M. A. 1984 Immune Modulation Agents and their Mechanisms. (Eds Fenichel, R. L. and Chirigos, M. A.) New York: M. DekkerGoogle Scholar
Klastrup, O. & Schmidt Madsen, P. 1974 [Scandinavian recommendations concerningexamination of udder samples for mastitis.] Nordisk Veterinaermedicin 26 197–204Google ScholarPubMed
Lee, C. S., Wooding, F. B. P. & Kemp, P. 1980 Identification, properties and differential counts of cell population using electron microscopy of dry cows secretion, colostrum and milk from normal cows. Journal of Dairy Research 47 39–50CrossRefGoogle ScholarPubMed
Mori, M. & Hayward, A. R. 1982 Phenotype and function of human milk monocytes as antigen presenting cells. Clinical Immunology and Immunopathology 23 94–99CrossRefGoogle ScholarPubMed
Ogra, S. S. & Ogra, P. L. 1979 Components of immunologie reactivity in human colostrum and milk. In Immunology of Breast Milk pp. 185–195 (Eds Ogra, P. L. and Dayton, D. H.), New York: Raven PressGoogle Scholar
Outteridge, P. M. & Lee, C. S. 1981 Cellular immunity in the mammary gland with particular reference to T, B lymphocytes and macrophages. In The Ruminant Immune System pp. 513–534. (Ed, Butler, J. E.). New York: Plenum Press (Advances in. Experimental Mediane and Biology 137)Google Scholar
Pitt, J. 1979 The milk mononuclear phagocyte. Pediatries 64, 745–749CrossRefGoogle ScholarPubMed
Raff, H. V., Cochrum, K. C. & Stobo, J. D. 1978 Macrophage-T cell interactions in the Con A induction of human suppressive T cells. Journal of Immunology 121 2311–2315CrossRefGoogle ScholarPubMed
Rosestreich, D. L., Farrar, J. J. & Dougherty, S. 1976 Absolute macrophage dependency of T lymphocyte activation by mitogens. Journal of Immunology 116 131–139CrossRefGoogle Scholar
Rosenwasser, L. J. 1986 Monocyte and macrophage function. In Manual of Clinical Laboratory Immunology pp. 321–325 (Eds Rose, N. L., Friedman, H. and Fahley, J. L.) Washington: American Society for MicrobiologyGoogle Scholar
Schore, C. E., Osburn, B. I., Jasper, D. E. & Tyler, D. E. 1981 B- and T-lymphocytes in bovine mammary gland: Rosette formation and mitogen response. Veterinary Immunology and Immunopathology 2 561–569CrossRefGoogle Scholar
Stevenson, H. C., Miller, P. J., Waxdal, M. J., Haynes, B. F., Thomas, C. A. & Fauci, A. S. 1983 Interaction of pokeweed mitogen with monocytes in the activation of human lymphocytes. Immunology 49 633–640Google ScholarPubMed
Unanue, E. R. 1981 The regulatory role of macrophages in antigenic stimulation. Part two: Symbiotic relationship between lymphocytes and macrophages. Advances in Immunology 31 1–136CrossRefGoogle ScholarPubMed
Unanue, E. R. 1984 Antigen-presenting function of the macrophage. Annual Reviews of Immunology 2 395–428CrossRefGoogle ScholarPubMed