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707. Phospholipids in New Zealand dairy products: I. The estimation of the total phospholipids and of lecithin, cephalin and sphingomyelin in butter

Published online by Cambridge University Press:  01 June 2009

A. K. R. McDowell
A. K. R. McDowell
Affiliation:
The Dairy Research Institute (N.Z.), Palmerston North, New Zealand
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Extract

A method has been described for the routine estimation of total phospholipids and of lecithin, cephalin and sphingomyelin in butter. Butter serum was treated with ether and light petroleum in a modified Röse-Gottlieb procedure to extract the phospholipids which then were estimated in the extract.

Chloroform-methanol extracted from the butter serum slightly more phosphorus than did the ether-light petroleum mixture. Ethanol-ether, benzene-ethanol and benzene-isopropanol, when used to extract a protein-phospholipid precipitate formed by the addition of trichloroacetic acid to butter serum, also removed slightly more phosphorus than did the ether-light petroleum mixture. The higher phosphorus contents of these extracts was due partly to a slightly more efficient extraction of phospholipids and partly to extraction of non-phospholipid phosphorus compounds.

Type
Original Articles
Information
Journal of Dairy Research , Volume 25 , Issue 2 , June 1958 , pp. 192 - 201
Copyright
Copyright © Proprietors of Journal of Dairy Research 1958

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