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473. The serological classification of Streptococcus cremoris

Published online by Cambridge University Press:  01 June 2009

C. A. E. Briggs  and
L. G. M. Newland
C. A. E. Briggs
Affiliation:
National Institute for Research in Dairying, University of Reading
L. G. M. Newland
Affiliation:
National Institute for Research in Dairying, University of Reading
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Extract

1. Attempts to produce in rabbits group N sera for Streptococcus cremoris, using suspensions of living, disintegrated and heat-treated bacterial cells, yielded only non-specific products, cross-reactions occurring most commonly and intensively with HCl extracts of the streptococci of groups K and L.

2. The non-specific antibodies in these sera were completely removed by absorption with streptococci of group K or L, the group N reactions being only partially impaired.

3. In order to inactivate, before immunizing rabbits, that antigen in the ‘cremoris’ cells responsible for stimulating the production of non-specific antibodies, the organisms were ‘absorbed’ with an excess of group K or L antiserum.

4. With suspensions of organisms treated in this way, potent group N specific precipitating sera were obtained for both freshly isolated and freeze-dried strains of Str. cremoris.

5. The identity of Str. cremoris with serological group N is thus established.

Type
Original Articles
Information
Journal of Dairy Research , Volume 19 , Issue 2 , June 1952 , pp. 160 - 166
Copyright
Copyright © Proprietors of Journal of Dairy Research 1952

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References

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