Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-26T23:57:06.987Z Has data issue: false hasContentIssue false
  • English
  • Français

31P-nuclear magnetic resonance study of milk fractions

Published online by Cambridge University Press:  01 June 2009

Magnus Wahlgren ,
Torbjörn Drakenberg ,
Hans J. Vogel  and
Petr Dejmek
Magnus Wahlgren
Affiliation:
Department of Food Technology, University of Lund, Box 124, S-221 00 Lund, Sweden
Torbjörn Drakenberg
Affiliation:
Department of Physical Chemistry 2, University of Lund, Box 124, S-221 00 Lund, Sweden
Hans J. Vogel
Affiliation:
Department of Physical Chemistry 2, University of Lund, Box 124, S-221 00 Lund, Sweden
Petr Dejmek
Affiliation:
Department of Food Technology, University of Lund, Box 124, S-221 00 Lund, Sweden
Get access Rights & Permissions [Opens in a new window]

Summary

Milk serum, whey and milk ultrafiltrate were examined by 31P nuclear magnetic resonance (31P-NMR). About 20 phosphorylated milk constituents gave rise to resonances in the spectra. Most of these have been assigned to such well-known milk constituents as inorganic phosphate, N-acetylglucosamine-1-phosphate and glycerophosphorylcholine. Resonances from previously unknown constituents such as phosphocreatine were also observed. When the pH-dependence of inorganic phosphate, N-acetylglucosamine-1 -phosphate, glycerophosphorylcholine and gly-cerophosphorylethanolamine was examined it was observed that the resonance of inorganic phosphate overlapped that of N-acetylglucosamine-1-phosphate around neutral pH. This is the most probable explanation as to why this constituent was not observed in earlier 31P-NMR studies on milk.

Type
Original Articles
Information
Journal of Dairy Research , Volume 53 , Issue 4 , November 1986 , pp. 539 - 545
Copyright
Copyright © Proprietors of Journal of Dairy Research 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Belton, P. S., Lyster, R. L. J. & Richards, C. P. 1985 The 31P nuclear magnetic resonance spectrum of cows' milk. Journal of Dairy Research 52 47–54CrossRefGoogle ScholarPubMed
Gadian, D. G. 1982 Nuclear Magnetic Resonance and its Applications to Living Systems. Oxford: Oxford University PressGoogle Scholar
Griffin, M. C. A. & Roberts, G. C. K. 1985 A 1H-n.m.r. study of casein micelles. Biochemical Journal 228 273–276CrossRefGoogle ScholarPubMed
Ho, C. & Kurland, R. J. 1966 Phosphorus nuclear magnetic resonance study of phosphoproteins. Journal of Biological Chemistry 244 3002–3007CrossRefGoogle Scholar
Ho, C., Magnusson, J. A., Wilson, J. B., Magnusson, N. S. & Kurland, R. J. 1969 Phosphorus nuclear magnetic resonance studies of phosphoproteins and phosphorylated molecules. II. Chemical nature of phosphorus atoms in αs-casein B and phosvitin. Biochemistry 8 2074–2082CrossRefGoogle Scholar
Humphrey, R. S. & Jolley, K. W. 1982 31P-NMR studies of bovine β-casein. Biochimica et Biophysica Acta 708 294–299CrossRefGoogle ScholarPubMed
O'Neill, I. K., Sargent, M. & Trimble, M. L. 1980 Determination of phytate in foods by phosphorus-31 Fourier transform nuclear magnetic resonance spectrometry. Analytical Chemistry 52 1288–1291CrossRefGoogle ScholarPubMed
Rollema, H. S., Vreeman, H. J. & Brinkhuis, J. A. 1984 1H-NMR Spectra of bovine κ-casein micelles. 22nd Ampère Congress on Magnetic Resonance and Related Phenomena 494–495 (Eds Muller, K. A., Kind, R. & Roos, J. E.). ZürichGoogle Scholar
Sleigh, R. W., Mackinlay, A. G. & Pope, J. M. 1983 NMR studies of the phosphoserine regions of bovine αs1-and β-casein. Biochimica et Biophysica Acta 742 175–183CrossRefGoogle ScholarPubMed
Visser, J., Schaier, R. W. & Van GORKOM, M. 1979 The role of calcium, phosphate and citrate ions in the stabilization of casein micelles. Journal of Dairy Research 46 333–335CrossRefGoogle ScholarPubMed
Vogel, H. J., Lundberg, P., Fabiansson, S., Ruderus, H. & Tornberg, E. 1985 Post-mortem energy metabolism in bovine muscles studied by non-invasive phosphorus-31 nuclear magnetic resonance. Meat Science 13 1–18CrossRefGoogle ScholarPubMed
Walstra, P. 1979 The voluminosity of bovine casein micelles and some of its implications. Journal of Dairy Research 46 317–323CrossRefGoogle ScholarPubMed
Walstra, P. & Jenness, R. 1984 Dairy Chemistry and Physics. New York: J. Wiley & SonsGoogle Scholar
Webb, B. H., Johnson, A. H. & Alford, J. A. 1974 Fundamentals of Dairy Chemistry, 2nd ednWestport. CT: Avi Publishing Co. Inc.Google Scholar