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258. The Vitamin B1 and Riboflavin of Milk

Published online by Cambridge University Press:  01 June 2009

J Houston ,
S. K. Kon  and
S.Y. Thompson
J Houston
Affiliation:
From the National Institute for Research in Dairying, University of Reading
S. K. Kon
Affiliation:
From the National Institute for Research in Dairying, University of Reading
S.Y. Thompson
Affiliation:
From the National Institute for Research in Dairying, University of Reading
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Extract

Part I

1. A method for the estimation of vitamin B1 in milk by a modified Jansen test involving takaphosphatase treatment is described in detail.

Part II

2. It is shown by ultra-filtration experiments, by treatment with pepsin and takaphosphatase, with trichloroacetic acid and with fuller's earth that vitamin Bx is present in milk unesterified and phosphorylated and that both forms may be freely diffusible or combined with protein either loosely or by firmer bonds.

Part III

3. The effect of the stage of lactation on the amounts and partition of vitamin B1 in milk has been studied. Samples of colostrum and early milk were found to contain large amounts of cocarboxylase (up to 80 % of the total). Hardly any cocarboxylase was present in mid or late lactation milks. The total vitamin B1 content was 60–100μg./100 ml. for colostrum, up to 60μg./100 ml. for early milk and 30–40μg./100 ml. for mid and late lactation.

4. A marked negative correlation exists between the cocarboxylase content of cow's and goat's milk and their alkaline phosphomonoesterase titre. As this enzyme cannot dephosphorylate cocarboxylase it is probably accompanied in milk by other enzymes more suited to this process.

5. The suggestion is put forward that the vitamin B1 of milk is mostly derived not directly from the blood but from the secretory cells of the mammary gland.

Type
Original Articles
Information
Journal of Dairy Research , Volume 11 , Issue 2 , May 1940 , pp. 145 - 183
Copyright
Copyright © Proprietors of Journal of Dairy Research 1940

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References

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