OBJECTIVES/SPECIFIC AIMS: There has been substantial research showing that there are race and sex differences on alcohol use. Similarly, race and sex disparities are also seen in a variety of different factors that impact drinking behaviors and other health outcomes. One of these factors of interest is Adverse Childhood Experiences (ACEs) which is associated with an increased risk for excessive alcohol use and the harmful effects of drinking. Several studies have shown that racial minorities and females have a greater risk of ACEs, which may be partly related to various structural factors (i.e. poverty) and social norms. Although there has been a substantial amount of research done on ACEs, very few studies have looked at how their interaction with race and sex can influence alcohol-related behaviors. METHODS/STUDY POPULATION: 1,509 participants who self-identified as either Black or White were recruited through a screening protocol at the NIAAA where they completed a series of questionnaires. We categorized the participants into two groups based on the Structured Clinical Interview for DSM-IV disorders: Alcohol Dependent individuals (N=921) with either a past and/or current diagnosis and Non-dependent individuals (N=588). ACEs exposure was assessed using the Childhood Trauma Questionnaire (CTQ). We looked at both total score and the 5 subscales: emotional abuse, physical abuse, sexual abuse, physical neglect, and emotional neglect. Drinking behaviors were assessed using a 90-day Timeline Followback interview and the Alcohol Use Disorder Identification Test (AUDIT). The non-dependent sample was 63% White and 55% male while the alcohol dependent sample was 47% White and 70% male. We tested the interaction effects using ANOVA. RESULTS/ANTICIPATED RESULTS: In the ND sample, there were significant race*sex*ACEs effects for average drinks per day with CTQ total score (P = 0.007), physical abuse (P = 0.005), and physical neglect (P = 0.003). There was also a 3-way interaction with physical neglect on heavy drinking days (P = 0.039) and a 2-way race*ACEs interaction on AUDIT total with physical abuse (P = 0.048). In the AD sample, there were significant 2-way race*ACEs interactions for three drinking outcomes: heavy drinking days with physical neglect (P = 0.009), AUDIT-Harmful Use subscore with CTQ total score (P = 0.028) and physical neglect (P = 0.001), AUDIT-Total score with CTQ total score (P = 0.007), physical abuse (P = 0.042), sexual abuse (P = 0.024), and physical neglect (P = 0.003). There were also 3-way interactions for AUDIT-Harmful use (P = 0.013) and AUDIT-Total scores (P = 0.011) with emotional abuse. DISCUSSION/SIGNIFICANCE OF IMPACT: Our results indicate that there are both 2-way (race*ACEs) and 3-way (race*sex*ACEs) interaction effects on alcohol consumption and the related negative effects for both non-dependent and dependent samples. There were no sex*ACEs interaction effects in either sample implying that race may play a bigger role in differentiating drinking outcomes by ACEs across males and females. However, contrary to our expectations, race seemed to be protective factor for Black participants against both alcohol consumption and the negative effects despite having higher rates of ACEs exposure. Future analyses will explore personality measures as potential mediators of the relationship between ACEs and alcohol use. Also, analyses will look to see if there are any behavioral factors that may contribute to resiliency among minority populations.