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Evidence for an interaction between linoleic acid intake and skin barrier properties in healthy dogs – a pilot study

Published online by Cambridge University Press:  21 September 2018

Adrian Watson ,
Gaelle Thomas ,
Christina Butowski  and
David Allaway
Adrian Watson*
Affiliation:
Royal Canin Research Centre, AimarguesFrance.
Gaelle Thomas
Affiliation:
Waltham Centre for Pet Nutrition, Leicestershire, UK.
Christina Butowski
Affiliation:
Waltham Centre for Pet Nutrition, Leicestershire, UK.
David Allaway
Affiliation:
Waltham Centre for Pet Nutrition, Leicestershire, UK.
*
§Corresponding author: [email protected]
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Abstract

Dogs suffer from skin associated issues with a disproportionate frequency, with consequent interest in providing nutrition to optimize their skin's natural defences. Linoleic acid (LA) is known as an essential nutrient in dogs and plays a critical part in the lipid component of skin barrier formation. Minimum requirements have been defined, primarily based on eliminating signs of deficiencies such as dry, flaky skin and inflammation. Zn has been shown as an important nutrient for maintaining epidermal health. This pilot study investigated whether there are skin barrier benefits from feeding both linoleic acid and Zn at levels significantly in excess of published minimum requirements. Eight Labrador retrievers were fed a diet containing 3.8 g/Mcal LA, 21 mg/Mcal Zn for 12 weeks to establish baseline conditions for all dogs (basal diet). After this period, for a further 12 weeks, the dogs were switched onto a diet containing 7.9 g/Mcal LA and 50 mg/Mcal Zn (test diet). Transepidermal water loss (TEWL measurements) were used as a measure of skin quality and integrity and were taken at the end of the initial feeding period, and at 6 and 12 weeks of the test feeding period. TEWL was reduced by 8.11 g/m2/h (P < 0.001) six weeks after instigation of the test diet, and by 7.52 g/m2/h (P < 0.005) at 12 weeks, compared to the levels (14.73 g/m2/h) at the end of the basal feeding trial period. The results showed evidence of improved barrier properties as TEWL when feeding higher levels of LA and Zn for six and 12 weeks.

Keywords

PUFATEWLepidermisceramidecanine
Type
Pilot Study
Information
Journal of Applied Animal Nutrition , Volume 6 , 2018 , e7
Copyright
Copyright © Cambridge University Press and Journal of Applied Animal Nutrition Ltd. 2018 

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References

Bauer, J.J.E. (2008) Essential fatty acid metabolism in dogs and cats. Revista Brasileira de Zootecnia [online access]. 37.Google Scholar
Bettger, W.J., Reeves, P.G., Moscatelli, E.A., Reynolds, G. and O'Dell, B.L. (1979) Interaction of Zn and essential fatty acids in the rat. Journal of Nutrition, 109:480–488.Google Scholar
Boelsma, E., Hendriks, H.F. and Roza, L. (2001) Nutritional skin care: health effects of micronutrients and fatty acids. American Journal of Clinical Nutrition, 73: 853–64.Google Scholar
Breiden, B. and Sandhoff, K. (2014) The role of sphingolipid metabolism in cutaneous permeability barrier formation. Biochimica Biophysica Acta, 1841: 441–52.Google Scholar
Conti, A., Rogers, J., Verdejo, P., Harding, C.R., Rawlings, A.V. (1996) Seasonal influences on stratum corneum ceramide 1 fatty acids and the influence of topical essential fatty acids. International Journal of Cosmetic Science, 18: 1–12.Google Scholar
du Plessis, J., Stefaniak, A., Eloff, F., John, S., Agner, T., Chou, T.C., Nixon, R., Steiner, M., Franken, A., Kudla, I. and Holness, L. (2013) International guidelines for the in vivo assessment of skin properties in non-clinical settings: Part 2. Transepidermal water loss and skin hydration. Skin Research and Technology, 19: 265–78.Google Scholar
Hansen, H.S. and Jensen, B. (1985) Essential function of LAesterified in acylglucosylceramide and acylceramide in maintaining the epidermal water permeability barrier. Evidence from feeding studies with oleate, linoleate, arachidonate, columbinate and alpha-linolenate. Biochimica Biophysica Acta, 834: 357–63.Google Scholar
Huang, Y.S., Cunnane, S.C., Horrobin, D.F. and Davignon, J. (1982) Most biological effects of Zn deficiency corrected by gamma-linolenic acid (18: 3 omega 6) but not by linoleic acid (18:2 omega 6). Atherosclerosis. 1982 Feb;41(2–3):193–207Google Scholar
Ishikawa, J., Shimotoyodome, Y., Ito, S., Miyauchi, Y., Fujimura, T., Kitahara, T. and Hase, T. (2013) Variations in the ceramide profile in different seasons and regions of the body contribute to stratum corneum functions. Archives of Dermatological Research, 305: 151–62.Google Scholar
MacDonald, M.L., Rogers, Q.R. and Morris, J.G. (1983) Role of linoleate as an essential fatty acid for the cat independent of arachidonate synthesis. Journal of Nutrition, 113:1422–1433.Google Scholar
Marsella, R. (2012) Are transepidermal water loss and clinical signs correlated in canine atopic dermatitis? A compilation of studies. Veterinary Dermatolology. 23: 238-e49.Google Scholar
Meguro, S., Arai, Y., Masukawa, Y., Uie, K. and Tokimitsu, I. (2000) Relationship between covalently bound ceramides and transepidermal water loss (TEWL). Archives of Dermatological Research, 292: 463–8.Google Scholar
National Research Council. (2006) Nutrient Requirements of Dogs and Cats. Washington, DC: The National Academies Press. https://doi.org/10.17226/10668Google Scholar
O′Neill, D.G., Church, D.B., McGreevy, P.D., Thomson, P.C. and Brodbelt, D.C. (2014) Prevalence of disorders recorded in dogs attending primary-care veterinary practices in England. PLoS ONE, 9: e90501.Google Scholar
Park, K. (2015) Role of micronutrients in skin health and function. Biomolecules & Therapeutics (Seoul), 23: 207–17.Google Scholar
Pinnagoda, J., Tupker, R.A., Smit, J.A., Coenraads, P.J. and Nater, J.P. (1989) The intra- and inter-individual variability and reliability of transepidermal water loss measurements. Contact Dermatitis, 21: 255–259.Google Scholar
Pinnagoda, J., Tupkek, R. A., Agner, T. and Serup, J. (1990) Guidelines for transepidermal water loss (TEWL) measurement. Contact Dermatitis, 22: 164–178.Google Scholar
Rogers, J., Harding, C., Mayo, A., Banks, J. and Rawlings, A. (1996) Stratum corneum lipids: the effect of ageing and the seasons. Archives of Dermatological Research, 288: 765–70.Google Scholar
Watson, A., Fray, T., Clarke, S., Yates, D. and Markwell, P. (2002) Reliable use of the ServoMed Evaporimeter EP-2 to assess transepidermal water loss in the canine. Journal of Nutrition, 132(6 Suppl 2): 1661S–4S.Google Scholar
Wei, K., Stella, C., Wehmeyer, K., Spruell, R. and Wickett, R. (2017) Effect of season on stratum corneum barrier function and skin surface biomarkers. Journal of the American Academy of Dermatology, 76 Supplement 1: AB108.Google Scholar
Wertz, P.W., Cho, E.S. and Downing, D.T. (1983) Effect of essential fatty acid deficiency on the epidermal sphingolipids of the rat. Biochimica Biophysica Acta, 753: 350–5.Google Scholar
Whelan, J and Fritsche, K. (2013) Nutrient Information. Linoleic Acid. Advances in Nutrition, 4: 311–312.Google Scholar