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Stereological estimation of volume-weighted mean glomerular volume from arbitrary sections of the equine kidney

Published online by Cambridge University Press:  19 October 2000

D. J. BEECH
Affiliation:
Fetal and Infant Toxico-Pathology, University of Liverpool, Liverpool, UK
E. D. ROCHE
Affiliation:
Fetal and Infant Toxico-Pathology, University of Liverpool, Liverpool, UK
P. D. SIBBONS
Affiliation:
Department of Surgical Research, Northwick Park Institute for Medical Research, Harrow, UK
P. D. ROSSDALE
Affiliation:
Rossdale and Partners, Newmarket, UK
J. C. OUSEY
Affiliation:
Rossdale and Partners, Newmarket, UK
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Abstract

Mean glomerular volume has previously been estimated, using stereological techniques, specifically the point-sampled intercept (PSI), either from isotropic or from vertical sections. As glomeruli are approximately spherical structures, the same stereological technique was carried out on vertical and arbitrary sections to determine whether section orientation had any effect on mean glomerular volume estimation. Equine kidneys from 10 individuals were analysed using the PSI method of estimating volume-weighted mean glomerular volume (MGV); for each kidney, arbitrary and vertical sections were analysed. MGVs were not significantly different between arbitrary and vertical sections (P = 0.691) when analysing the data with the paired t test; when plotting MGV estimates from arbitrary sections against those from vertical sections the intercept was found not to be significantly different from zero (P > 0.8) and the slope of the regression line not to be significantly different from 1.0 (P > 0.4). For the estimation of MGV in equine kidneys using PSI, arbitrary sections may be used if it is not possible to use isotropic or vertical sections, but some caution must be exercised in the interpretation of results so gained.

Type
Short Report
Copyright
© Anatomical Society of Great Britain and Ireland 2000

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