The possibilities for increasing total grain yield in organic cereal production through manipulation of crop rotation design were investigated in a field experiment on different soil types in Denmark from 1997 to 2000. Three experimental factors were included in the experiment in a factorial design: (1) proportion of grass-clover and pulses in the rotation, (2) catch crop (with and without) and (3) manure (with and without). Three four-course rotations were compared. Two of the rotations had 1 year of grass-clover as a green manure crop, either followed by spring wheat or by winter wheat. The grass-clover was replaced by winter cereals in the third rotation. Animal manure was applied as slurry in rates corresponding to 40% of the nitrogen (N) demand of the cereal crops.

Rotational grain yields of the cereal and pulse crops were calculated by summing yields for each plot over the 4 years in the rotation. The rotational yields were affected by all experimental factors (rotation, manure and catch crop). However, the largest effects on both dry matter and N yields were caused by differences between sites caused by differences in soils, climate and cropping history. The rotation without a green manure crop produced the greatest total yield. Dry matter and N yields in this rotation were about 10% higher than in the rotation with a grass-clover ley in 1 year in 4. Therefore, the yield benefits from the grass-clover ley could not adequately compensate for the yield reduction as a result of leaving 25% of the rotation out of production. There were no differences in dry matter and N yields in grains between the rotations, where either spring or winter cereals followed the grass-clover ley. The N use efficiency for ammonium-N in the applied manure corresponded to that obtained from N in commercial fertilizer. There were only very small yield benefits from the use of catch crops. However, this may change over time as fertility builds up in the system with catch crops.

The effects of straw incorporation (early and late cultivation) and straw burning were contrasted in a split-plot study examining the impact of long-term straw residue management, and six fertilizer nitrogen (N) rates on soil mineral nitrogen, crop fertilizer N requirements and nitrate leaching losses. The experiments ran from 1984 to 1997 on light-textured soils at ADAS Gleadthorpe (Nottinghamshire, UK) and Morley Research Centre (Norfolk, UK).

Soil incorporation of the straw residues returned an estimated 633 kg N/ha at Gleadthorpe and 429 kg N/ha at Morley on the treatment receiving 150 kg/ha per year fertilizer N since 1984. Straw disposal method had no consistent effect on grain and straw yields, crop N uptake, or optimal fertilizer N rates. In every year there was a positive response (P<0·001) to fertilizer N in straw/grain yields, N contents and crop N offtakes at both sites. Nitrate leaching losses were slightly reduced by less than 10 kg N/ha where straw residues had been incorporated, while fertilizer N additions increased nitrate leached at both sites.

At both sites there was a consistent effect (P<0·001) of straw disposal method on autumn soil mineral N, with values following the pattern burn>early incorporate>late plough. The incorporation of straw residues induced temporary N immobilization compared with the treatment where straw was burnt, while the earlier timing of tillage on the incorporate treatment resulted in slightly more mineral N compared with the later ploughed treatment. Fertilizer N rate increased (P<0·001) soil mineral nitrogen at both sites. At Morley, there was more organic carbon in the plough layer where straw had been incorporated (mean 1·09 g/100 g) rather than burnt (mean 0·89 g/100 g), and a strong positive relationship between organic carbon and fertilizer N rate (r2=93·2%, P<0·01). There was a detectable effect of fertilizer N on readily mineralizable N in the plough layer at both Gleadthorpe (P<0·001) and Morley (P<0·05). At Morley, there was a consistent trend (P=0·06) for readily mineralizable N to be higher where straw had been incorporated rather than burnt, indicating that ploughing-in residues may contribute to soil nitrogen supply over the longer term.

The effects of ploughing or no-tillage of long-term grass and grass-clover swards on changes in organic C and N pools and on CO2 and denitrified gas emissions were investigated in a 3-year field experiment in 1996–99 near Penicuik, Scotland. The decrease in soil C content between 1996 and 1999 was 15·3 t/ha (95% confidence limits were 1·7–28·9 t/ha). Field estimates of CO2 losses from deep-ploughed, normal-ploughed and no-tillage plots were 3·1, 4·5 and 4·6 t/ha over the sampling periods (a total of 257 days) in 1996–98. The highest N2O fluxes were from the fertilized spring barley under no-tillage. Thus no-tillage did not reduce C emissions, caused higher N2O emissions, and required larger inputs of N fertilizer than ploughing. By contrast, deep ploughing led to smaller C and N2O emissions but had no effect on yields, suggesting that deep ploughing might be an appropriate means of conserving C and N when leys are ploughed in. Subsoil denitrification losses were estimated to be 10–16 kg N/ha per year by measurement of 15N emissions from incubated intact cores. A balance sheet of N inputs and outputs showed that net N mineralization over 3 years was lower from plots receiving N fertilizer than from plots receiving no fertilizer.

Supplying a proportion of the N requirement of a wheat crop via the foliage would potentially reduce immobilization of fertilizer N in the soil organic matter and N losses by leaching or denitrification. A field experiment was carried out at Harper Adams in Shropshire to investigate the effect on crop yield of supplying the spring N application to winter wheat as different proportions of urea as a solution rather than as conventional soil-applied urea, and to determine the physiological basis of any yield differences. A solid ammonium nitrate treatment was included to represent alternative commercial practice to solid urea. Treatments were repeated on the same plots over the 3 years 1992, 1993 and 1994. Solid fertilizer was applied as a single dressing, whereas urea sprays were split over a number of days to reduce scorch. Nitrogen as urea sprays produced similar grain yields to N applied conventionally to the soil as solid ammonium nitrate or urea, but effects on above-ground dry matter production and harvest index depended on the time of application. Application of a large proportion of N as urea sprays, such that some of the N as urea solution was applied later in relation to crop development, produced less above-ground dry matter, but compensated by increasing harvest index. It is concluded that application of N as urea sprays could be successfully used to substitute for soil-applied N fertilizer at stem extension in winter wheat without loss of yield. Extra application costs, however, are likely to outweigh any efficiency or environmental benefits, except where applications of solid N are made to dry soils.

The effects of supplying the fertilizer nitrogen (N) as a recommended quantity of ammonium nitrate or as a commonly used dose of poultry manure on yield of sugarbeet infected with Beet mild yellowing virus (BMYV) or Beet yellows virus (BYV) were studied in field experiments at IACR-Broom's Barn in 1990, 1991 and 1992. Three N fertilizer treatments comprising Zero (N0), standard rate of 110 kg N/ha (N1) and poultry manure equivalent to c. 300 kg/ha of available N (N2) were applied to plots which were uninoculated or were subsequently inoculated with either BMYV or BYV. Averaged over virus treatments, N1 increased sugar yields by 23% relative to N0: there was no further increase when N2 was applied. When averaged over N treatments, early virus yellows infection reduced the sugar yields by 23%. Generally there was no significant interaction between N supply and virus infection. There was no evidence that the large N supply could reduce the yield effect of virus yellows infection, as had previously been thought. Crops infected from late July produced similar yields to uninoculated controls. The main effect of virus yellows was to reduce the efficiency of radiation conversion even when account was taken of the light intercepted by yellow foliage. Whilst the N2 treatment helped to maintain a green leaf cover throughout the season on virus yellows infected crops, it had no effect on virus replication. Beet processing quality was impaired by increasing the N supply and by virus infection, but again there were generally no significant interactions between infection and N rate.

A field experiment was carried out at Harper Adams in Shropshire to investigate the effect of supplying the spring N application to winter wheat as different proportions of urea as a solution, rather than as conventional soil-applied solid urea, on N recovery in the above-ground crop, autumn soil mineral N and nitrate leaching over the subsequent winter. A solid ammonium nitrate treatment was also included to represent alternative commercial practice to solid urea. Treatments were repeated on the same plots over the 3 years 1992, 1993 and 1994. N recovery was measured in all 3 years by difference in N uptake between fertilized and unfertilized plots, and in 1993 for selected treatments, N was applied as 15N-labelled fertilizer to determine direct uptake of fertilizer N in the crop and soil. Both urea sprays and solid soil N applications were labelled with 15N. Urea sprays were split over several days to reduce scorch, whereas solid fertilizer was applied as a single dressing. For some urea spray treatments, apparent N recovery in the above-ground crop in 1992 and 1994 was less compared with soil-applied N treatments. These urea spray treatments were applied in the morning rather than the evening, and gaseous losses, most likely by volatilization, are suggested. In 1992 application of a large proportion of N as urea sprays, such that application of some N as urea solution was delayed to around GS 37, was associated with an increase in physiological N use efficiency. In 1993, there was no difference in direct or apparent recovery of fertilizer N in the crop or soil for N applied as ammonium nitrate, solid urea or as urea sprays. Mean nitrate concentration in the drainage water at 1 m was elevated for all N treatments in all years, but only in 1992 did nitrate concentration and leaching loss decrease with increasing proportion of N applied as urea sprays. It may therefore be possible to reduce gaseous losses by application of urea sprays under cool conditions in the evening and exploit the increased physiological N use efficiency for urea sprays applied later, such that total fertilizer N applied and N losses are reduced.

A 3-year field experiment was conducted in 1996–98 near Penicuik, Scotland, to investigate the fate of N released after cultivation of previously long-term grass and grass-clover swards. The effects of timing of cultivations (autumn and spring), tillage methods (no tillage, ploughing to 200 mm and ploughing to 300 mm) and fertilizer N for spring (0, 40, 80 and 120 kg N/ha) and winter barley (0, 60, 120, 180 kg N/ha) on yield, N uptake and nitrate leaching were measured.

In 1996, after spring cultivation, on plots previously in grass, spring barley grain yield and N uptake did not respond to N fertilizer, but on plots previously in grass-clover there was a nearly linear response to N. In 1997, the spring barley responded to N fertilizer at all levels. Yields of 1997 winter barley after grass did not show a response above 60 kg N/ha, but increased with fertilizer N up to at least 120 kg N/ha after grass-clover. In 1998, there were strong effects of N fertilizer and cultivation method on grain yield and N uptake of both spring barley and winter barley. Winter barley grain yield was significantly higher in plots previously in grass than in plots previously in grass-clover in 1998, though not in 1997. Winter barley yields were higher than spring barley at the same fertilizer N level.

Throughout the 3 years, the no-tillage plots had consistently lower yields than the ploughed plots, but there was no consistent difference between the ploughed and deep ploughed treatments. There were strong interactive effects between tillage and previous sward in 1997. No-tillage under spring barley generally yielded lower than ploughing due to difficulties in weed control and the frequent anaerobic conditions in the soil.

Annual leaching losses were relatively small (6·4–19·6 kg N/ha). In 1996–97, more N was leached from the plots left in stubble following spring barley than from those planted with winter barley after either spring barley or grass in 1996, but in 1997–98 more N was leached from plots in winter barley than from those in over-winter stubble. Nitrate leaching was least under no-tillage, though the effect was not significant.

Small-scale silos (60-litre polyethylene barrels) were used to examine the chemical composition of grass silage after the application of slurry, at 40 m3/ha, at a range of timings, at two sites with contrasting rainfall; North Wyke, in Devon (high rainfall) and Reaseheath College, Cheshire (low rainfall). Grass plots (60 m2) received cattle slurry via surface broadcast (S), shallow injection (I) or surface placement by trailing shoe (T) techniques at 10, 6 or 2 weeks before an early silage (first) cut in May/June, and 6 or 2 weeks before a second cut in June/July. Control plots (C) received no slurry treatment. The harvested grass was compressed and sealed in the experimental silos and samples of the herbage at ensiling and of ensilage (after >100-day fermentation) were analysed for various chemical components. Herbage yield was measured when the harvests were taken. Wetter than average weather conditions in both years resulted in silages with variable DM content (range 140–277 g/kg) being made at both sites. Generally, silages were poorly preserved with high pH and NH4+-N and low lactic acid contents. In particular, the silages from treatment S where slurry was applied 2 weeks prior to harvest exhibited characteristics indicative of clostridial growth, with high pH (range 4·0–5·2) and NH4+-N (106–213 g/kg total N) and low lactic acid (16–86 g/kg DM) contents. Damage to herbage caused by the injection tines on taller swards (treatments I at 6 and 2 weeks before cutting) was evident and the injection process was impeded by tall grass. This contributed to herbage contamination with soil and slurry on these treatments and, consequently, to poor silage fermentation. Silages made with slurry application method T at all timings showed improved fermentation characteristics compared with the other treatments, particularly at North Wyke. The results indicate that silage quality is unlikely to be compromised by slurry applications made in early spring, during February, March or even in April, where these are at agronomically sensible rates. Such practice will enable better utilization of slurry nitrogen (N), reducing risks of nutrient losses via leaching or surface runoff. Furthermore, shallow injection and, particularly, trailing shoe slurry application techniques may be used to increase flexibility of slurry management by allowing more immediate spreading before cutting than with conventional surface broadcasting, without detriment to silage quality.

Twelve perennial ryegrass (Lolium perenne L.) varieties of different ploidy and maturity classifications were compared under a frequent cutting management in their second harvest year, equivalent to the simulated rotational grazing system employed in UK testing protocols. Varietal differences in canopy structure (proportion of lamina, green leaf mass, sward surface height, extended tiller height, bulk density) and in herbage nutritive value factors (water-soluble carbohydrate content and proportion of linoleic and α-linolenic fatty acids) were assessed and their importance evaluated with reference to total herbage production. Significant variety variation (P<0·001) was recorded in the annual means of all the canopy structure characteristics. Significant differences associated with ploidy were also recorded, with tetraploid varieties having significantly higher values than diploids in most plant characters, indicating better intake characteristics for these grasses. Temporal patterns of variation associated with maturity were also observed in several characters, thus making it impossible to designate a single assessment that would be representative of the annual ranking of varieties. Water-soluble carbohydrate concentration differed significantly (P<0·001) between varieties and although the tetraploids tended to have high contents, the highest value of all was recorded in a diploid variety, which had been selectively bred for this trait. The varieties did not differ in total lipid content but there were significant differences in the proportion of linoleic acid between varieties (P<0·001) while the proportion of α-linolenic acid differed between varieties (P<0·001), ploidy (P<0·001) and maturity (P<0·05) classes.

Overall evaluation of the extensive variety variation highlighted the need for better quantification of animal responses to differences of these magnitudes, before the high workload of including them in routine variety testing protocols could be justified. Potential for breeding improvement in these factors was also indicated and the future prospects for their use in farmer decision support systems was considered.

This study compared phosphorus (P) speciation and the relationship between bicarbonate extractable (Olsen) P and 0.01 M CaCl2 extractable P (a measure of potentially mobile P) in soils from plots of the Park Grass experiment started in 1856 at IACR-Rothamsted, UK and with and without nitrogen as (NH4)2SO4 and with and without calcium carbonate (CaCO3, lime). A point, termed the change point, was noted in Olsen P, above which 0.01 M CaCl2-P increased at a greater rate per unit increase in Olsen P than below this point. Previous findings have shown a change point for soils with a pH>5.8 at 56 mg Olsen P/kg and at 120 mg Olsen P/kg for soils below this pH. Soils given (NH4)2SO4 annually since 1856 and with lime periodically since 1903 mostly had a pH between 3.7 to 5.7, some of these (NH4)2SO4 treated soils were limed to pH 6.5 and above from 1965. Irrespective of their pH in 1991/92 all the soils had a similar change point (120 mg Olsen P/kg) to that found for other soils with pH<5.8 (112 mg Olsen P/kg). In a laboratory study lasting 30 days, the addition of CaCO3 to acid soils from the field experiment that had received (NH4)2SO4 had a similar change point to soils with pH<5.8 irrespective of pH, suggesting soil P chemistry was controlled by the long period of soil acidity and this was not reversed by a short period at a higher pH. The effect of pH was attributed to the creation of P sorptive surfaces on aluminium precipitates compared with less acidic soils (pH>5.8) where there was less exchangeable Al to be precipitated. This was confirmed with solid-state 31P nuclear magnetic resonance, which indicated that for soils of similar total P concentration and pH, there was twice as much amorphous Al-P in soils given (NH4)2SO4 compared with those without. Changes in pH as a result of applications of (NH4)2SO4 or lime can greatly change the concentration of potentially mobile P due to the effects on Al solubility. Although there was less potentially mobile P in soils with pH<5.8 than in soils above this pH, it is usually advised in temperate regions to maintain soils about pH 6.5 for arable crops.

In vivo digestibility trials were carried out using six young rams fed with Atriplex halimus biomass harvested in summer (A) and in early autumn (B), and with a mixture of mid-autumn Atriplex halimus and wheat straw (5[ratio ]1 ratio on fresh matter basis) (C). Atriplex halimus had a high protein content (139·0, 135·9 and 193·4 g/kg DM in A, B and C respectively), but was rich in sodium chloride, especially in summer (145·9 g/kg DM), limiting its use as feed. The summer forage had a higher organic matter (OM) digestibility coefficient than the autumn forage (0·663 v. 0·530) but lower digestible OM intake (16·8 v. 29·4 g/day per kg BW0·75). In autumn forage, the combination with straw did not influence the digestibility of organic matter, whereas it enhanced DOM intake in comparison with the Atriplex halimus on its own (35·7 v. 29·4 g/day per kg BW0·75).

Two systems for integrated weed control in winter wheat based around the combination of herbicides with cultural control have been investigated and compared with conventional practice in experiments between 1993 and 2001. These systems were (a) an overall spray of a reduced herbicide dose followed by spring tine harrow weeding and (b) the combination of herbicide applied over the crop row with a novel vision guided inter-row hoe. The latter required wheat to be established with a wider (22 cm) inter-row spacing than standard (12·5 cm). Experiments over 10 sites/seasons indicated that this increased spacing could be achieved without yield loss. Trials to measure the accuracy of hoe blade lateral positioning using the vision guidance system indicated that error was normally distributed with standard deviation of 12 mm and a bias that could be set to within 1 cm. This performance could be maintained through the normal hoeing period and the crop row location and tracking techniques were robust to moderate weed infestation. In the absence of weeds neither overall harrowing nor inter-row hoeing affected winter wheat yield, 1000-seed weight or specific weight in 12·5 or 22 cm rows. When combined with inter-row hoeing, manually targeted banded applications of fluazolate, pendimethalin or isoproturon reduced grass weed levels and increased yields over untreated controls, though better results were obtained using overall herbicides. However, improvements would be possible with more accurately targeted herbicide applications and more effective inter-row grass weed control. The implications and costs of using such an integrated system are discussed and requirements for future developments identified.

This work evaluated the effect of seed size and morphology on the development and biomass of durum wheat seedlings. Three different seed-grading sizes selected by sieving were used in glasshouse experiments, and a set of three developmental and 23 biomass-related indices were measured on eight genotypes, at two moisture levels. The influence of seed size on seedling development was studied at high and low temperatures (22/12 °C, and 15/5 °C day/night temperatures, respectively), in growth chambers.

The area of the seed and the area of the embryo were the seed morphological traits most affected by seed size. Seed size was strongly associated with seedling development and seedling biomass until the complete extension of the first two leaves, at the fourth leaf stage. The rate of first-leaf growth and the area of the first leaf were the developmental and biomass traits, respectively, most sensitive to seed-grading size.

Relationships between leaf expansion and MeOH-soluble (cytosol) and cell-wall fractions, and their sugar composition prior to silking in flint corn lines were studied. A greater husk leaf area of one genotype, X-15 is mainly due to prolonged and higher rate of expansion. Prior to rapid expansion of husk leaf area, neutral sugars in the cytosol fraction accounted for most of the non-starch carbohydrates (56–62%), while hemicellulose and cellulose fractions accounted for less than 20%. In mature leaf parts, however, sugars in the cytosol fraction decreased but those in hemicellulose and cellulose fractions increased by 30% and 42%, respectively. The predominant sugar in the cytosol fraction was glucose (Glc), while in the hemicellulose fraction xylose (Xyl) and arabinose (Ara) dominated. During rapid expansion of husk leaves, 13C was incorporated at a higher rate into hemicellulose than cellulose, and this process was more active in X-15 than in other genotypes. During an identical period, 13C atom % excess in Xyl increased markedly in the hemicellulose fraction, however it remained low in the cytosol one. The current results suggest that synthesis of Xyl and xylan plays an important role in renewal of hemicellulose, which may be required for expansion.

A greenhouse experiment was carried out from January–May 2001, to evaluate the effects of elemental sulphur inoculated with Thiobacillus, compared with gypsum, in the amendment of two saline sodic soils (Neosol Fluvic Salic sodic) from the Brazilian semi-arid region, and on growth of the tropical legumes cowpea and yam bean, inoculated with specific rhizobia strains. The treatments consisted of sulphur rates (0·6, 1·2 and 1·8 t/ha) and gypsum (1·8 and 3·6 t/ha), and irrigation water containing the salts NaHCO3, MgCl2, CaCl2, NaCl and KCl, with electrical conductivity 0·2 dS/m at 25 °C. There was a treatment with no sulphur or gypsum applied. The correctives increased Na+ and electrical conductivity on leached solution, and decreased soil pH and exchangeable cations, especially Na+. Sulphur inoculated with Thiobacillus was more efficient than gypsum, reducing soil-exchangeable sodium. Sulphur with Thiobacillus in Soil 1 reduced pH (8·2 to 4·7) and electrical conductivity of the soil saturation extract (15·3 to 1·7 mS/cm) to values below those used for classification as saline and sodic soil. The growth of the tropical legumes cowpea and yam bean was increased by rhizobia inoculation when soil ameliorants were used, especially sulphur in combination with Thiobacillus.

Each crop variety has a genotype-specific ability to maintain performance over a wide range of environmental conditions. This ability is usually referred to as the sensitivity or adaptability of a variety. Such an ability is an important property, because farmers naturally want to use varieties which perform well in their own fields. Assessing sensitivity has, however, proved difficult, because of problems involved in defining and measuring the wide diversity of natural environments. These problems often lead to split statistical analyses of trial data or statistical models including explanatory variables with no biological interpretation. That causes ambiguity in statistical inference and prediction. The present study shows how the latest advances in statistical research can be applied to overcome some of these difficulties. A key point is to use the conditional expectation of the yield given the environment as a latent explanatory variable. In this way the predicted yields of different varieties can be estimated at any expected environmental yield level. Discussion is restricted to yield data but similar methods can be applied to other performance characters. The Finnish statutory variety trial data are used to illustrate the methods and the results.

The marginal winter pastures of reindeer and caribou (Rangifer tarandus) in Arctic and sub-Arctic areas are assumed to be nitrogen- and mineral-poor. Reindeer eat a mixed diet consisting of nitrogen-deficient lichens and a wide variety of vascular plants in winter. Some reindeer populations manage to maintain carcass mass outside the growing season and very little is known about the ability of reindeer to utilize vascular plants in this season. The chemical composition and in vitro digestibility of 17 species of vascular forage plants and one species of moss collected mid-winter from beneath the snow at an inland winter pasture in northern Norway were determined. Wintergreen parts of graminoids had a high content of crude protein (0.072–0.108 g/kg dry matter (DM)) and water-soluble carbohydrates (0.098–0.167 g/kg DM) and were highly digestible (50–65% IVDMD) compared with the withered parts of the plants (27–53% IVDMD). The digestibility of both shrubs and graminoids was inversely related to content of cellulose and lignin, but positively correlated with increasing contents of water-soluble carbohydrates. Shrubs were relatively calcium-rich (3.6–6.1 g/kg DM) while wintergreen graminoids contained up to 10.0 g/kg DM of potassium, 1.5 g/kg DM magnesium and 2.1 g/kg DM phosphorus. The present study demonstrates that the wintergreen parts of several species of vascular plants are nutritious, containing high levels of nitrogen and minerals, and that they are highly digestible to reindeer in winter. Vascular plants may, therefore, be more important to reindeer and caribou in winter than previously realized.

No genetic estimates for resistance to Mal de Río Cuarto (MRC) disease in Zea mays (L.) are currently available in the literature. Therefore, the objectives of this investigation were (i) to estimate the variance and heritability of partial resistance to MRC disease and of other agronomic traits from maize families and (ii) to examine associations among MRC disease severity values across different environments and between MRC and other agronomic traits. These estimations, obtained in an endemic area, could contribute to the design of efficient enhancement programmes and evaluation activity for the improvement of MRC resistance. The research was conducted by testing 227 F3 derived-lines from a cross between a susceptible dent line, Mo17, and a partially resistant flint line, BLS14, for MRC disease at two Río Cuarto locations in each of 2 years. The resistance of the lines, measured with a disease severity index (DSI), was normally distributed across environments. Genotypic variances were highly significant on all scoring environments. Estimates of genotype–environment interaction were also significant, suggesting that certain genotypes have little stability over different environments. For disease severity index all estimates demonstrated moderate heritabilities ranging from 0.44 to 0.56 and were similar when based on individual environments or across environment. Confidence interval widths ranged from 34.88 to 50.30% as large as the heritability point estimate. The correlations between environments were small enough to indicate that families did not rank similarly in individual environments for MRC resistance. Disease severity index correlated significantly (P<0.01) with plant height, leaf surface, leaf border, leaf length and tassel type. Heritability estimates for plant height and tassel type were 0.48 and 0.38 respectively and for the various leaf traits heritability values were very low. On the basis of the substantial genotype–environment interaction and the little association between DSI values in the different environments, selection for an increased resistance to MRC disease would require evaluation of germplasm across multiple years and locations. Tassel type would be a useful predictor of DSI and can be used effectively to improve screening procedures.

Trifolium species are native to the Mediterranean Basin where they are an important component of permanent pastures. A number of species have become naturalized in Australia and are agriculturally important in annual pastures. To understand the importance of genetic and ecotypic variation in the adaptation of Trifolium species to a new environment, seeds of three predominantly inbreeding and two predominantly outcrossing species of Trifolium were collected from 12 sites in Sardinia in 1998. Detailed ecogeographic information was collected at each site. The progeny were grown at the University of Western Australia Field Station at Shenton Park, Western Australia in 1999, and were scored for 10 morphological characters. Spatial analysis was applied to the data to improve the estimation of accession means, and to investigate the relationship with environmental variables that characterized the sites of collection. The spatially adjusted means were used as the basis for a principal components analysis. Ecogeographical factors at the sites of collection, particularly soil pH, are suggested to be more important than breeding system in determining the extent of genetic variation within the species. The species that showed the greatest genetic variation between accessions were the predominantly inbreeding species T. glomeratum and T. subterraneum, and the predominantly outcrossing species T. nigrescens. It is suggested that the wide genetic variation of these three species is largely due to their being in an ecogeographic environment close to their optimum, and to the possession of a mating system that is neither completely outcrossing nor completely self-fertilizing. The remaining two species, T. tomentosum and T. resupinatum, both occur more frequently on alkaline soils, and it is suggested that for the collected accessions of these two species the acid soils of Sardinia are a stressful environment that does not promote high levels of genetic variation.

Field experiments were conducted at the Crop Research Centre of Govind Ballabh Pant University of Agriculture and Technology, Pantnagar during 1996/97 and 1997/98. Each experiment comprised 10 crop sequences: (a) wheat–rice, (b) chickpea–rice, (c) lentil–rice, (d) pea–rice, (e) wheat–mungbean green manure–rice, (f) wheat–Sesbania green manure–rice, (g) wheat–fodder–rice, (h) chickpea–fodder–rice, (i) lentil–fodder–rice and (j) pea–fodder–rice, in a randomized block design with four replications. The crop sequences were compared in terms of economic rice equivalent yield (REY), protein production, apparent nutrient balances and effect on soil fertility status. Amongst crop sequences involving two crops each year (200% cropping intensity), chickpea–rice gave highest REY and protein production. Of the sequences involving three crops each year (300% cropping intensity), chickpea–fodder–rice and wheat–fodder–rice were superior to others. The P balances were positive for all sequences, whereas K balances were generally negative except for sequences involving green manure legumes. Green manuring with Sesbania or mungbean helped restore soil fertility, indicating the advantage of green manure for higher productivity and sustainability of rice–wheat system. Chickpea–rice and chickpea–fodder–rice appeared promising alternatives to rice–wheat crop sequence.