The influence of herbicides at reduced rates and repeated stubble-cultivation on weeds and crop yields was estimated in five field trials with spring-sown cereals situated in the south of Sweden during the autumn of 1989 until the spring of 1997. Stubble-cultivation was accomplished during 1989–1996, while herbicides were applied at 0, 1/8, 1/4 or 1/2 of full dose during 1990–1996.

In the spring of 1997, i.e. after 7 years without herbicide application, seedling densities 3 weeks after weed emergence were 68–340/m2 at three sites and 535–610/m2 at two sites when averaged over tillage treatments.

Averaged over herbicide doses, stubble-cultivation reduced the plant density of annual broad- leaved weeds by 6–32% at three sites and increased the density by 25% at one site. At the remaining site, the density was not significantly influenced. Stubble-cultivation reduced the populations of two perennial and seven annual weed species, while one species was stimulated and nine species showed null, or inconsistent, responses. In the spring of 1997, i.e. one year after the last herbicide application, the densities of weed seedlings in 1/8, 1/4 and 1/2-doses were 34, 46 and 56% lower, respectively, than in the untreated controls.

Stubble-cultivation increased crop yields at four sites by 200 kg/ha as a mean over herbicide doses. At these four sites, averaged over 1993–1995, herbicides increased yields in plots that were not stubble-cultivated by 7, 8 and 10% in the 1/8, 1/4 and 1/2 of a full dose, respectively, relative to the untreated control. In 1996, herbicides increased yields at only two sites.

It is concluded that a fruitful way for weed management with a low input of agrochemicals is to combine the use of herbicides at reduced rates with repeated stubble-cultivation.

Data from three ryegrass spaced plant variety trials conducted at Crossnacreevy, Co. Down in each of 1989, 1990 and 1991 were investigated for evidence of spatial dependence. Spatial dependence was most evident in variates measuring the overall dimensions of the plants, especially late season variates. However, the ‘date of ear emergence’ variate, which identifies most of the variety distinctnesses that the trials are intended to detect, was not spatially dependent. On average, 45% of the residual variation in the plot means of the spatially dependent variates was estimated to be of a spatial nature. Consequently, it is likely that the efficiency of analysis of these variates would be improved by using spatial analysis instead of non-spatial analysis or by using alpha (incomplete block) designs instead of complete block designs. The spatial information was used to determine the optimal incomplete block sizes and the likely increase in efficiency due to using alpha designs instead of complete block designs. The optimal incomplete block size for a trial with 10 plants per plot was compared with the optimal incomplete block size for the same trial with single plant plots at the same spacing and was found to be smaller. The gains in efficiency were larger in the trial with 10 plants per plot than in the trial with single plant plots.

Two organic fertilizers were prepared from the same initial mixture of poplar sawdust, blood and flour either by composting in a reactor or by chemical oxidation. Both processes resulted in loss of c. 30% of the organic matter. Composting required 90 days in comparison to only a few hours with chemical oxidation. Extraction of the organic residues with 1 N KOH gave solutions containing 24·6 and 15·1 g/l of humic substances respectively. These humic solutions were applied to pot-grown Lolium multiflorum Lam. at 4 and 10 mg carbon per pot to assess the short-term uptake of macro and microelements by the plants. When the plants were short of phosphorus, the humic substances from the chemically decomposed sawdust supplied at 10 mg C per pot improved total P uptake and yield. Humic substances also increased copper and manganese uptake, and by enhancing root development, also improved nitrogen uptake and biomass yield.

Laboratory incubation in the UK of an Oxisol from Burundi and an Ultisol from Cameroon with 3·1% by weight of prunings of young shoots of Calliandra calothyrsus, Cassia siamea, Flemingia congesta, Grevillea robusta, Gliricidia sepium, Leucaena diversifolia and Leucaena leucocephala resulted in increased soil pH and decreased exchangeable aluminium content. The greatest increase in pH and corresponding decrease in exchangeable aluminium occurred during the first 14 days of incubation and the decrease continued at a slower rate until 42 days incubation. The acid neutralizing effect decreased after 42 days but was still important at the last sampling time at 98 days. Polyphenol to nitrogen ratio was not well correlated with observed change in soil pH whereas the total base cation (calcium, magnesium and potassium) content proved to be a good predictor of these changes in the Ultisol, but not in the Oxisol. The proposed mechanism giving rise to acid neutralization is complexation of protons and aluminium by organic anions. The total base cation content of the prunings ranged from 0·94 to 2·25 molc/kg and the buffer capacity of the Oxisol was 48 mmol OH−/pH/kg.

A 5-year study was conducted from 1988 to 1992 at three sites in Niger to determine the effects of crop rotation of a cereal and legumes and nitrogen fertilizer on chemical properties of the soil (0–20 cm) and yield of pearl millet (Pennisetum glaucum (L.) R.BR.), cowpea (Vigna unguiculata (L.) Walp.), and groundnut (Arachis hypogea L.). Four N levels and rotation treatments including continuous fallow were investigated. Soil samples taken from the top 20 cm depth at the end of the experiment from treatments without nitrogen application which included continuous fallow, fallow–millet rotation, groundnut–millet rotation, cowpea–millet rotation, and continuous millet were analysed for soil pH, organic carbon, total nitrogen and exchangeable bases. Fertilizer N significantly increased yield of pearl millet, cowpea and groundnut. Continuous monocropping of pearl millet resulted in lower yields across N levels compared to legume–millet rotations. Legume yields were also consistently lower in monoculture than when rotated with millet. There was a decline in organic matter under continuous millet, cowpea–millet rotation and groundnut–millet rotation. The fallow–millet rotation supplied more mineral N than the legume–millet rotations. Nitrogen availability was greater in cowpea–millet rotation than continuous millet. Crop rotation was more productive than the continuous monoculture but did not differ in maintaining soil organic matter. The legume–millet rotation at 30 kg/ha N appears to be the most viable for millet production. Research should focus on understanding the effect of legume/cereal intercrops and rotations on soil productivity.

Field investigations were conducted during the winter seasons of 1992/93 and 1993/94 to find the optimum sowing time and time of last fodder cut for the fodder and seed production of Egyptian clover (Trifolium alexandrinum L.). The delay in sowing from 1 October to 15 November decreased the green and dry fodder yields but increased the seed yields. Total green and dry fodder yields (of all cuttings) for 1 or 15 October sowings were significantly higher than the 15 November sowing in both years and 1 November sowing in 1992/93. Increase in seed yield from the 15 November sowing was greater than from the 1 and 15 October sowings. Straw yields followed similar trends. Delay in the last cut for fodder, however, increased green and dry fodder yields but decreased the seed yield. The percentage increase in dry fodder yield with each successive delay in last cut for fodder was higher than that of green fodder yield. The 11 April cutting date of fodder produced the highest green and dry fodder yields but the lowest seed yield. Two years mean data showed that the 2 March cutting date of last fodder gave the highest seed yield. It is concluded that higher seed yield of Egyptian clover can be produced by sowing on 15 November and giving the last fodder cutting on 2 March.

A collection of 64 accessions of the South American pasture legume Aeschynomene histrix Poiret was characterized in 1995 at Ibadan in south-west Nigeria in order to test the potential of the accessions as forage plants in subhumid areas and as a trap crop for the parasitic plant Striga hermonthica (Del.) Benth. All accessions had a low biomass production during the first 8 weeks after sowing but soon variations in the collection became apparent. Morphologically, accessions could be classified as having prostrate, decumbent, semi-erect and erect growth habit. The semi-erect to erect accessions grew vigorously and had high dry matter yields. The most frequent disease was Rhizoctonia foliar blight, for which, in general, prostrate accessions had a higher severity. In contrast, plants with semi- erect to erect growth habit were more susceptible to a wilting disease probably caused by Fusarium spp. With an average crude protein content of 16% and 48-h in sacco dry matter and protein digestibilities of c. 50%, fodder quality of 13-week-old plants was similar to that of other tropical forage legumes. As a result of a laboratory experiment to test the potential of A. histrix to act as a trap crop for Striga hermonthica, 13 accessions showing a significant influence on in vitro Striga hermonthica seed germination were identified. Twenty promising accessions were selected for further evaluation. These accessions showed good biomass production and/or high tolerance to diseases and/or significant influence on Striga hermonthica seed germination. Such material has the potential to contribute to both livestock-forage and improved fallow systems with the aim of increasing the sustainability of agricultural production in West Africa.

In situ and in vitro rumen incubations were used to determine the effect of condensed tannins (CT) on the solubilization and degradation of the plant protein from white clover (Trifolium repens) and Lotus corniculatus. These forages contained, respectively 0·3 and 22·1 g CT/kg dry matter (DM). The sheep used for the experiments were also fed either white clover or L. corniculatus. Effects of CT were determined by making measurements in the presence and absence of polyethylene glycol (PEG; molecular weight 3500), which binds and inactivates CT. The loss of DM, neutral detergent fibre (NDF), total nitrogen (N) and Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase; EC 4.1.1.39; fraction I leaf protein) from polyester bags suspended in the rumen of sheep was measured. The loss of these constituents from polyester bags suspended in the rumen was used as a measurement of their solubilization. Degradation was defined as the disappearance of Rubisco from white clover and L. corniculatus added to in vitro incubations with rumen fluid obtained from the same fistulated sheep fed either white clover or L. corniculatus.

In the absence of PEG, the in situ loss of Rubisco from L. corniculatus was less rapid than the loss of this protein from white clover when each forage was incubated in the rumen of sheep fed the same diet. Addition of PEG tended to increase the loss of Rubisco from L. corniculatus, suggesting that CT slowed the rates of solubilization of Rubisco from this forage. Effects of rumen fluid were small, but there was some evidence that the rumen fluid in sheep fed L. corniculatus reduced the solubilization of Rubisco from white clover. The action of CT did not inhibit the in situ loss of NDF from either white clover or L. corniculatus.

In the absence of PEG, the in vitro degradation of Rubisco from L. corniculatus was slower when compared to the degradation of this protein from white clover; PEG addition increased the degradation of Rubisco from L. corniculatus, but not from white clover, showing that CT was the causal agent. The addition of CT extracted from L. corniculatus markedly depressed the degradation of Rubisco from white clover, with the effect being completely reversible by PEG. The large subunit (LSU) of Rubisco was consistently degraded at a faster rate than the small subunit (SSU) and added CT had a greater effect in slowing the degradation of the LSU compared to the SSU. There was little difference in the degradation of Rubisco when rumen fluid from sheep fed either white clover or L. corniculatus was used for in vitro incubations.

It was concluded that the action of CT from L. corniculatus reduces the digestion of protein in the rumen of sheep. This effect is predominantly due to the action of CT reducing the degradation of plant protein, although CT also reduced the solubilization of plant protein. The main effects of CT on protein solubilization and degradation seemed to be produced locally by CT present in plant tissue; transfer of these effects through rumen fluid was small in magnitude.

An isotope dilution method using [U-13C]glucose and a glucose clamp approach were applied to determine the effects of supplemental chromium (Cr) and heat exposure on blood glucose metabolism and tissue responsiveness and sensitivity to insulin in sheep. The sheep consumed diets with either 0 or 1 mg of Cr/kg (Control and +Cr diet, respectively) from high-Cr-yeast, and were exposed from a thermoneutral environment (20 °C) to a hot environment (30 °C) for 5 days. Blood glucose turnover rate did not differ between the diets, and was lower (P < 0·05) during heat exposure than in the thermoneutral environment. The maximal glucose infusion rate (tissue responsiveness to insulin) tended to be lower (P = 0·06) for the +Cr diet than for the Control diet, but did not change with heat exposure. The plasma insulin concentration at half maximal glucose infusion rate (tissue sensitivity to insulin) did not differ between the diets, and was greater (P < 0·05) during heat exposure than in the thermoneutral environment. No significant diet × environment interactions were observed. There was no significant evidence that Cr supplementation moderated heat stress in sheep from the measures of blood glucose metabolism and insulin action.

Of the anthropogenic greenhouse gas emission in Denmark animal manure contributes an estimated 40% of methane (CH4) and 20% of nitrous oxide (N2O). Livestock production systems undergo changes for the purpose of increasing animal welfare, and such changes often include increasing the amounts of bedding manure. Emission of greenhouse gases from composting pig deep litter was studied during a 4-month period. Effects of increasing the amount of straw used in deep litter (reducing litter density) were included in the study. Methane was produced at a high rate in the centre of the heap at high density during the thermophilic phase of composting, and CH4 emission was only measured during this phase. In this treatment N2O was also produced in the centre both initially and after the temperature of the compost had dropped to below 45 °C. Emissions of N2O were only significant in the low temperature phases. Production of N2O was probably restricted to the surface layers during the thermophilic phase of composting. Total carbon dioxide (CO2) emissions were 7·37 and 0·09 kg C/t fresh weight from the heaps with bulk densities of 0·44 and 0·23 kg/l, respectively. Methane emission from the high density compost heap was 191 g C/t and N2O emission was 58 g N/t. Emissions of CH4 and N2O from the low density heap were not detected. The greenhouse effect of gas emission from the high density compost heap was calculated by multiplying the climate force efficiencies and the cumulated gas emission. This calculation showed that CH4 contributes almost as much to the global warming potential as CO2, and N2O contributed twice as much as CO2.