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Variation in PLIN2 and its association with milk traits and milk fat composition in dairy cows

Published online by Cambridge University Press:  30 March 2021

Y. H. Li ,
H. Zhou Open the ORCID record for H. Zhou [Opens in a new window] ,
L. Cheng ,
J. Zhao  and
J. G. H. Hickford
Y. H. Li
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
H. Zhou
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
L. Cheng
Affiliation:
Faculty of Veterinary and Agricultural Sciences, Dookie College, The University of Melbourne, Victoria3647, Australia
J. Zhao
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
J. G. H. Hickford*
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
*
Author for correspondence: J. G. H. Hickford, E-mail: [email protected]
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Abstract

The current study investigated associations between variation in the bovine perilipin-2 gene (PLIN2) and milk traits (milk fat content, milk protein content, milk yield and milk fatty acid (FA) component levels) in 409 New Zealand pasture-grazed Holstein-Friesian × Jersey-cross (HF × J-cross or Kiwicross™) cows. Five nucleotide sequence variants were found in three regions of the gene, including c.17C>T in exon 2, c.53A>G in exon 3, c.595+23G>A and c.595+104_595+108del in intron 5, and c.*302T>C in the 3′-untranslated region. The c.*302T>C substitution produces two nucleotide sequence variants (A5 and B5), and this variation was associated with variation in milk protein content and milkfat composition for C10:0, C11:0, C12:0, C13:0 and C16:0 FA and medium-chain fatty acid (MCFA) and long-chain fatty acid (LCFA) groups. After correcting for the effect of variation in the diacylglycerol acyl-CoA acyltransferase 1 gene (DGAT1) that results in the amino acid substitution p.K232A, variation in the FA binding protein 4 gene (FABP4) and variation in the stearoyl-CoA desaturase (Δ-9-desaturase) gene (SCD) that results in the amino acids substitution p.A293V, significant differences between A5A5 and B5B5 cows were found for C10:0, C11:0, C12:0, C13:0, C16:0, and the MCFA, LCFA, total saturated FA and C10:1 index groups. This suggests that nucleotide sequence variation in PLIN2 may be affecting milk FA component levels.

Keywords

Genetic variationmilk fatty acidPLIN2
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 158 , Issue 8-9 , November 2020 , pp. 774 - 780
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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