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Sperm production of a pony stallion and the treatment of spermatozoa in vitro with special reference to artificial insemination of mares

Published online by Cambridge University Press:  27 March 2009

Min Chueh Chang
Min Chueh Chang
Affiliation:
School of Agriculture, University of Cambridge
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Extract

1. The sperm production of a pony stallion kept a constant high level when the collection of sperms was performed regularly three times a week. There was no adverse effect on the stallion or on the quantity and quality of the sperms. There is a negative correlation between the volume of semen and the concentration. The total number ejaculated remains relatively constant; the volume of accessory fluids is more variable.

2. The motility of horse spermatozoa after dilution with eight different kinds of chemical media and stored at different temperatures was studied: Glucose-yolk-phosphate dilutor devised by Lamhert & McKenzie and glucose-yolk-tartrate dilutor devised by the author were found to be the best for the preservation of horse sperms at low temperature. There was not much difference between those diluted samples stored for 24 hr. at 10° C. and those slowly cooled to 1° C. Concentration of the semen by centrifuge is definitely beneficial for the preservation of horse sperms.

3. Pregnancies were obtained by the insemination of sperms centrifuged and kept at 1° C. for 24 hr. The sperms of one stallion can be used for a great number of mares if artificial insemination is practised. The adoption of new techniques for the determination of the time of ovulation and for the induction of ovulation is suggested for successful artificial insemination of mares.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 33 , Issue 2 , April 1943 , pp. 67 - 73
Copyright
Copyright © Cambridge University Press 1943

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References

REFERENCES

Berliner, V. R., Cowart, F. E., Means, R. H. & Wright, J. B. (1938). Proc. Amer. Soc. Anim. Prod. 31, 233.Google Scholar
Chang, M. C. (1941). A study of the physiology of ram spermatozoa. Ph.D. Thesis, Cambridge University, unpublished.Google Scholar
Chang, M. C. & Walton, A. (1940). Proc. Roy. Soc. B, 129, 517.Google Scholar
Davis, G. K. & Cole, C. L. (1939). Proc. Amer. Soc. Anim. Prod. 32, 81.Google Scholar
Day, F. T. (1940 a). Vet. Rec. 52, 597.Google Scholar
Day, F. T. (1940 b). J. Agric. Sci. 30, 244.CrossRefGoogle Scholar
Day, F. T. (1942). J. Agric. Sci. 32, 108.Google Scholar
Hartman, G. G. (1939). Sex and Internal Secretions, ed. Allen, E., 2nd ed. pp. 630. Baltimore: Williams and Wilkins.Google Scholar
Laing, J. A. (1943). J. Agric. Sci. 33, 64.Google Scholar
Lambert, W. V. & McKenzie, F. F. (1940). Circ. U.S. Dep. Agric. no. 567.Google Scholar
Lardy, H. A. & Phillips, P. H. (1939). Proc. Amer. Soc. Anim. Prod. 32, 219.Google Scholar
MoKenzie, F. F. (1940). Proc. Amer. Soc. Anim. Prod. 33, 98.Google Scholar
McKenzie, F. F., Lasley, J. F. & Phillips, R. W. (1939). Proc. Amer. Soc. Anim. Prod. 32, 222.Google Scholar
Milovanov, V. K., Lihacev, A. N. & Zevanova, T. A. (1939). Sovetsk. Zooteh. 4, 31. (Anim. Breed. Abs. 8, 110, 1940.)Google Scholar
Polowzow, W. (1928). Pflüg. Arch. ges. Physiol. 218, 374.Google Scholar
Skatein, P. N. (1940). C.R. Acod. Sci. U.R.S.S. 8, 29. (Anim. Breed. Abs. 8, 344, 1940.)Google Scholar
Walton, A. (1938). Proc. Amer. Soc. Anim. Prod. 31, 238.Google Scholar
Walton, A. (1942). Notes on Artificial Insemination of Sheep, Cattle and Horses, 3rd ed. London: Holborn Instrument Co.Google Scholar
Woloskow, P. A. (1936). Probl. Anim. Husb. 2, 69. (Anim. Breed. Abs. 4, 285, 1936.)Google Scholar
Yamane, J. (1938). On the Status of Studies on the Artificial Insemination of the Domestic Animal in Japan. Kyoto, Japan.Google Scholar