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Some properties of thiaminases associated with cerebrocortical necrosis

Published online by Cambridge University Press:  27 March 2009

E. E. Edwin ,
R. Jackman  and
P. Jones
E. E. Edwin
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey, KT15 3NB
R. Jackman
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey, KT15 3NB
P. Jones
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey, KT15 3NB
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Summary

Thiaminases from different sources exhibit varied responses to change in pH and to differing activators, cosubstrates and inhibitors. These properties are used in an attempt to characterize the extracellular thiaminases of 17 strains of Bacillus spp. isolated from the rumen contents of animals affected with cerebrocortical necrosis and to compare these with the endogenous thiaminase present in the rumen content of such animals and with other known thiaminase-producing bacteria. Statistical analysis of the results obtained using different activators classified the isolates into two main groups corresponding to the presence of thiaminase I or thiaminase II. The pH characteristics of the rumen content enzyme differed significantly from any of the bacterial isolates examined.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 99 , Issue 2 , October 1982 , pp. 271 - 275
Copyright
Copyright © Cambridge University Press 1982

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References

Boyd, J. W. & Walton, J. R. (1977). Cerebrocortical necrosis in ruminants: an attempt to identify the source of thiaminase in affected animals. Journal of Comparative Pathology 87, 581–589.CrossRefGoogle ScholarPubMed
Cowan, S. T. & Steel, K. J. (1974). Manual for the Identification of Medical Bacteria, 2nd edn.Cambridge University Press.Google Scholar
Edwin, E. E. & Jackman, R. (1973). Ruminal thiaminase and tissue thiamine in cerebrocortical necrosis. Veterinary Record 92, 640–641.CrossRefGoogle ScholarPubMed
Edwin, E. E. & Jackman, R. (1974). A rapid radioactive method for determination of thiaminase activity and its use in the diagnosis of cerebrocortical necrosis in sheep and cattle. Journal of the Science of Food and Agriculture 25, 357–368.CrossRefGoogle ScholarPubMed
Edwin, E. E., Jackman, R., Machin, A. F. & Quick, M. P. (1976). The importance of Δ1-pyrroline in the aetiology of cerebrocortical necrosis. Biochemical and Biophysical Research Communications 70, 1190–1197.CrossRefGoogle ScholarPubMed
Edwin, E. E., Markson, L. M., Shreeve, J., Jackman, R. & Carroll, P. J. (1979). Diagnostic aspects of cerebrocortical necrosis. Veterinary Record 104, 4–8.CrossRefGoogle ScholarPubMed
Kobayashi, S. (1975). Studies on the thiaminase from Clostridium sporogenes. Vitamins (Japan) 49, 111–119.Google Scholar
Morgan, K. T. & Lawson, G. H. K. (1974). Thiaminase type I producing bacilli and ovine polioencephalomalaoia. Veterinary Record 95, 361–363.CrossRefGoogle Scholar
Roberts, G. W. & Boyd, J. W. (1974): Cerebrocortical necrosis in ruminants. Occurrence of thiaminase in the gut of normal and affected animals and its effect on thiamine status. Journal of Comparative Pathology 84, 365–374.CrossRefGoogle ScholarPubMed
Rowett Research Institute (1978). Annual Report, p. 52.Google Scholar
Shreeve, J. & Edwin, E. E. (1974). Thiaminaseproducing strains of Clostridium sporogenes associated with outbreaks of cerebrocortical necrosis. Veterinary Record 94, 330.CrossRefGoogle Scholar