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A simple and rapid method of quantitatively measuring the glucosinolate concentration of rapeseed

Published online by Cambridge University Press:  27 March 2009

D. B. Smith ,
D. G. Parsons  and
Carol Starr
D. B. Smith
Affiliation:
Plant Breeding Institute, Maris Lane, Trumpington, Cambridge, GB2 2LQ
D. G. Parsons
Affiliation:
Plant Breeding Institute, Maris Lane, Trumpington, Cambridge, GB2 2LQ
Carol Starr
Affiliation:
Plant Breeding Institute, Maris Lane, Trumpington, Cambridge, GB2 2LQ
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Summary

A method is described in which glucosinolates are simultaneously extracted from crushed seeds and degraded by myrosinase. Glucose released by myrosinase is measured by an autoanalyser using a single reagent.

Using conditions optimized for samples of 200 mg, yields were slightly greater than those obtained from corresponding samples of defatted meal. Coefficients of variation of about 4% were obtained over a range of more than 100 μmol of glucosinolate/g seed. It is suggested that the method will be of value to plant breeders selecting from populations having low and intermediate concentrations of glucosinolate and to others wishing to obtain a rapid but precise measurement of these important compounds.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 105 , Issue 3 , December 1985 , pp. 597 - 603
Copyright
Copyright © Cambridge University Press 1985

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References

REFERENCES

Adams, H., Vaughan, J. G. & Fenwick, G. R. (1985). The limited chemotaxonomic value of glucosinolates for cultivar identification in Brassica napus L. var. oleifera (Metzg.) Zeitschrift für Pflanzenzüchtung (in the Press).Google Scholar
Appelqvist, L. A. (1967). Further studies on a multisequential method for determination of oil content in oilseeds. Journal of the American Oil Chemists Society 44, 209–214.CrossRefGoogle Scholar
Craig, E. A. & Morgan, A. G. (1980). A modified method for the quantitative determination of total glucosinolate in rapeseed. Analytical Chemistry of Rapeseed and its Products (ed. Daun, J. K., McGregor, D. I. and McGregor, E. E.), pp. 81–85. Winnipeg: The Canola Council of Canada.Google Scholar
Fenwick, G. R., Heaney, R. K. & Mullin, W. J. (1983). Glucosinolates and their breakdown products in food and food plants. CRC Critical Reviews of Food Science and Nutrition 18, 123–201.CrossRefGoogle ScholarPubMed
McGregor, D. I. & Downey, R. K. (1975). A rapid and simple assay for identifying low glucosinolate rapeseed. Canadian Journal of Plant Science 55, 191–196.CrossRefGoogle Scholar
McGregor, D. I., Mullin, W. J. & Fenwick, G. R. (1983). Analytical methodology for determining glucosinolate compositions and content. Journal of the Association of Official Analytical Chemists 66, 825–849.Google Scholar
Martland, M. F., Butler, E. J. & Fenwick, G. R. (1984). Rapeseed induced liver haemorrhage, reticulolysia and biochemical changes in laying hens: the effects of feeding high and low glucosinolate meals. Research in Veterinary Science 36, 298–309.CrossRefGoogle ScholarPubMed
Sang, J. P. & Truscott, R. J. W. (1984). Liquid chromatographic determination of glucosinolates in rapeseed as desulfoglucosinolates. Journal of the Association of Official Analytical Chemists 67, 829–833.Google Scholar
Thompson, K. F. (1983). Breeding winter oilseed rape (Brassica napus). Advances in Applied Biology 7, 1–104.Google Scholar
Van Etten, C. H., Gagne, W. E., Robbins, D. J., Booth, A. N., Daxenbichler, M. E. & Woltt, I. A. (1969). Biological evaluation of Crambe seed meals and derived products by rat feeding. Cereal Chemistry 46, 145–155Google Scholar
Wilkinson, A. P., Rhodes, M. J. C. & Fenwick, G. R. (1984). Myrosinase activity of cruciferous vegetables. Journal of the Science of Food and Agriculture 35, 543–552.CrossRefGoogle Scholar
Worthington, C. C. & Teller, J. D. (1959). U.S. Patent 2, 850,359.Google Scholar