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Seed treatment with glycine betaine enhances tolerance of cotton to chilling stress

Published online by Cambridge University Press:  23 April 2018

C. Cheng ,
L. M. Pei ,
T. T. Yin  and
K. W. Zhang
C. Cheng
Affiliation:
School of Life Science, Shandong University, Jinan 250100, China Ministry of Education Key Laboratory of Plant Cell Engineering and Germplasm Enhancement, Jinan, 250100, China
L. M. Pei
Affiliation:
School of Biological Science and Technology, University of Jinan, Jinan, 250022, China
T. T. Yin
Affiliation:
School of Food Science and Engineering, Qi Lu University of Technology, Jinan, 250353, China
K. W. Zhang*
Affiliation:
School of Life Science, Shandong University, Jinan 250100, China Ministry of Education Key Laboratory of Plant Cell Engineering and Germplasm Enhancement, Jinan, 250100, China
*
Author for correspondence: K. W. Zhang, E-mail: [email protected]
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Abstract

Chilling injury is an important natural stress that can threaten cotton production, especially at the sowing and seedling stages in early spring. It is therefore important for cotton production to improve chilling tolerance at these stages. The current work examines the potential for glycine betaine (GB) treatment of seeds to increase the chilling tolerance of cotton at the seedling stage. Germination under cold stress was increased significantly by GB treatment. Under low temperature, the leaves of seedlings from treated seeds exhibited a higher net photosynthetic rate (PN), higher antioxidant enzyme activity including superoxide dismutase, ascorbate peroxidase and catalase, lower hydrogen peroxide (H2O2) content and less damage to the cell membrane. Enzyme activity was correlated negatively with H2O2 content and degree of damage to the cell membrane but correlated positively with GB content. The experimental results suggested that although GB was only used to treat cotton seed, the beneficial effect caused by the preliminary treatment of GB could play a significant role during germination that persisted to at least the four-leaf seedling stage. Therefore, it is crucial that this method is employed in agricultural production to improve chilling resistance in the seedling stage by soaking the seeds in GB.

Keywords

Antioxidant enzymeschilling stresscotton seedsglycine betainephotosynthesis
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 156 , Issue 3 , April 2018 , pp. 323 - 332
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Copyright
Copyright © Cambridge University Press 2018 

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