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Scanning electron microscopy observations of pollen morphology in common vetch (Vicia sativa) subspecies

Published online by Cambridge University Press:  07 January 2021

Rui Dong ,
Zhongjie Lu Open the ORCID record for Zhongjie Lu [Opens in a new window] ,
Zhengyu Yang ,
Yanrong Wang  and
Chao Chen
Rui Dong
Affiliation:
Department of Grassland Science, College of Animal Science, Guizhou University, Guiyang, Guizhou550025, China
Zhongjie Lu
Affiliation:
Department of Grassland Science, College of Animal Science, Guizhou University, Guiyang, Guizhou550025, China
Zhengyu Yang
Affiliation:
Department of Grassland Science, College of Animal Science, Guizhou University, Guiyang, Guizhou550025, China
Yanrong Wang
Affiliation:
State Key Laboratory of Grassland Agro-ecosystems, China, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou730020, China
Chao Chen*
Affiliation:
Department of Grassland Science, College of Animal Science, Guizhou University, Guiyang, Guizhou550025, China
*
Author for correspondence: Chao Chen, E-mail: [email protected]
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Abstract

Common vetch (Vicia sativa) is an important forage and green manure crop that is widely cultivated around the world. However, the large number of subspecies and accessions of common vetch has made taxonomic research on this species difficult. Pollen morphology data can provide important evidence in the study of plant phylogeny. Therefore, in this research, light microscopy and scanning electron microscopy were used to observe seven morphological traits of pollens from 22 common vetch accessions, and residual maximum likelihood and pattern analysis was conducted. The results showed that the pollen grains of the 22 accessions were all monad pollen and the polar view revealed three-lobed circular and triangular shapes, while the equatorial view mainly revealed an oblate shape; only one accession showed an oblate spherical shape. All accessions were 3-zonocolporate and the colpus length extended close to the poles. The polar axis length was (19.39 ± 0.97)–(42.12 ± 0.76) μm and the equatorial axis length was (35.97 ± 1.27)–(45.25 ± 0.81) μm. We found that qualitative traits of pollen shape, aperture polar view and ornamentation were highly stable. Among them, polar axis length, equatorial axis length and colpus length and width had significant differences among the accessions. The ratios of the equatorial and polar axes had significant differences among the subspecies. This trait information could be used for the classification and identification of common vetch accessions and subspecies.

Keywords

Legumemicroscopic morphologypattern analysis
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 158 , Issue 8-9 , November 2020 , pp. 646 - 659
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

Joint first authors.

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