Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-24T01:52:38.040Z Has data issue: false hasContentIssue false
  • English
  • Français

Scanning electron microscopy observations of pollen morphology in common vetch (Vicia sativa) subspecies

Published online by Cambridge University Press:  07 January 2021

Rui Dong ,
Zhongjie Lu Open the ORCID record for Zhongjie Lu [Opens in a new window] ,
Zhengyu Yang ,
Yanrong Wang  and
Chao Chen
Rui Dong
Affiliation:
Department of Grassland Science, College of Animal Science, Guizhou University, Guiyang, Guizhou550025, China
Zhongjie Lu
Affiliation:
Department of Grassland Science, College of Animal Science, Guizhou University, Guiyang, Guizhou550025, China
Zhengyu Yang
Affiliation:
Department of Grassland Science, College of Animal Science, Guizhou University, Guiyang, Guizhou550025, China
Yanrong Wang
Affiliation:
State Key Laboratory of Grassland Agro-ecosystems, China, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou730020, China
Chao Chen*
Affiliation:
Department of Grassland Science, College of Animal Science, Guizhou University, Guiyang, Guizhou550025, China
*
Author for correspondence: Chao Chen, E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Common vetch (Vicia sativa) is an important forage and green manure crop that is widely cultivated around the world. However, the large number of subspecies and accessions of common vetch has made taxonomic research on this species difficult. Pollen morphology data can provide important evidence in the study of plant phylogeny. Therefore, in this research, light microscopy and scanning electron microscopy were used to observe seven morphological traits of pollens from 22 common vetch accessions, and residual maximum likelihood and pattern analysis was conducted. The results showed that the pollen grains of the 22 accessions were all monad pollen and the polar view revealed three-lobed circular and triangular shapes, while the equatorial view mainly revealed an oblate shape; only one accession showed an oblate spherical shape. All accessions were 3-zonocolporate and the colpus length extended close to the poles. The polar axis length was (19.39 ± 0.97)–(42.12 ± 0.76) μm and the equatorial axis length was (35.97 ± 1.27)–(45.25 ± 0.81) μm. We found that qualitative traits of pollen shape, aperture polar view and ornamentation were highly stable. Among them, polar axis length, equatorial axis length and colpus length and width had significant differences among the accessions. The ratios of the equatorial and polar axes had significant differences among the subspecies. This trait information could be used for the classification and identification of common vetch accessions and subspecies.

Keywords

Legumemicroscopic morphologypattern analysis
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 158 , Issue 8-9 , November 2020 , pp. 646 - 659
Check for updates
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

Joint first authors.

References

Abozeid, A, Liu, Y, Liu, J and Tang, ZH (2017) Comparative foliar structure of Vicia L. Species from China. Journal of Biosciences and Medicines 5, 170–175.CrossRefGoogle Scholar
Bahadur, S, Ahmad, M, Mir, S, Zafar, M, Sultana, S, Ashfaq, S and Arfan, M (2018) Identification of monocot flora using pollen features through scanning electron microscopy. Microscopy Research and Technique 81, 599–613.CrossRefGoogle ScholarPubMed
Ball, PW (1968) Flora Europaea, vol. 2. Cambridge: The University Press, 129–136.Google Scholar
Banks, H and Lewis, G (2018) Phylogenetically informative pollen structures of ‘caesalpinioid’ pollen (Caesalpinioideae, Cercidoideae, Detarioideae, Dialioideae and Duparquetioideae: Fabaceae). Botanical Journal of the Linnean Society 187, 59–86.CrossRefGoogle Scholar
Beck, CB (1981) The Origin of Angiosperms and Early Evolution. Beijing: Science Press, 190–194.Google Scholar
Cakmakci, S, Aydinoglu, B, Karaca, M and Bilgen, M (2006) Heritability of yield components in common vetch (Vicia sativa L.). Acta Agriculturae Scandinavica, Section B – Soil & Plant Science 56, 54–59.Google Scholar
Chen, MJ and Jia, SX (2002) Chinese Forage Plants. Beijing: China Agriculture Press, 673–675. (in Chinese).Google Scholar
Chung, JW, Kim, TS, Suresh, S, Lee, SY and Cho, GT (2013) Development of 65 novel polymorphic cDNA-SSR markers in common vetch (Vicia sativa Subsp. sativa) using next generation sequencing. Molecules 18, 8376–8392.CrossRefGoogle ScholarPubMed
Dong, R, Jahufer, MZZ, Dong, DK, Wang, YR and Liu, ZP (2016) Characterisation of the morphological variation for seed traits among 537 germplasm accessions of common vetch (Vicia sativa L.) using digital image analysis. New Zealand Journal of Agricultural Research 59, 1–14.CrossRefGoogle Scholar
Endo, Y and Ohashi, H (1996) The Pollen Morphology of Vicia (Leguminosae). American Journal of Botany 83, 955–960.CrossRefGoogle Scholar
Fehr, WR (1987) Principles of Cultivar Development, vol. 1. New York: Collier Macmillan Publishers.Google Scholar
Firincioglu, HK (2014) A comparison of six vetches (Vicia Spp.) for developmental rate, herbage yield and seed yield in semi-arid central Turkey. Grass Forage Sci 69, 303–314.10.1111/gfs.12021CrossRefGoogle Scholar
Gabriel, KR (1971) The biplot graphical display of matrices with application to principal component analysis. Biometrika 58, 453–467.CrossRefGoogle Scholar
Jaaska, V (1997) Isoenzyme diversity and phylogenetic affinities in Vicia Subgenus Vicia (Fabaceae). Genetic Resources and Crop Evolution 44, 557–574.CrossRefGoogle Scholar
Jaaska, V (2005) Isozyme variation and phylogenetic relationships in Vicia Subgenus Cracca (Fabaceae). Annals of Botany 96, 1085–1096.CrossRefGoogle Scholar
Jafar, E and Karm, A (2007) Polynological study of some visited medicinal plants by honey bee in Fars province. Iranian Journal of Medicinal and Aromatic Plants 22, 420–430.Google Scholar
Jahufer, MZZ, Da, C, Nichols, S, Crush, J, Li, O and Dunn, A (2006) Phenotyping and pattern analysis of key root morphological traits in a white clover mapping population. Advances in pasture plant breeding: papers from the 13th Australasian Plant Breeding Conference, 8–21.10.33584/rps.12.2006.3031CrossRefGoogle Scholar
Kahraman, A, Binzat, OK and Doğan, M (2013) Pollen morphology of some taxa of Vicia L. subgenus Vicia (Fabaceae) from Turkey. Plant Systematics And Evolution 299, 1749–1760.CrossRefGoogle Scholar
Kroonenberg, PM (1994) The TUCKALS line: a suite of programs for three-way data analysis. Computational Statistics & Data Analysis 18, 73–96.CrossRefGoogle Scholar
Kupicha, FK (1976) The infrageneric structure of Vicia. L. Notes Royal Botanical Garden Edinburgh 34, 287–326.Google Scholar
Lee, S, Heo, KI, Cho, J, Lee, C, Chen, W and Kim, SC (2011) New insights into pollen morphology and its implications in the phylogeny of Sanguisorba L. (Rosaceae; Sanguisorbeae). Plant Systematics And Evolution 291, 227–242.CrossRefGoogle Scholar
Leht, M and Jaaska, V (2002) Cladistic and phenetic analysis of relationships in Vicia subgenus Vicia (Fabaceae) by morphology and isozymes. Plant Systematics and Evolution 232, 237–260.CrossRefGoogle Scholar
Liu, ZP, Ma, LC, Nan, ZB and Wang, YR (2013) Comparative transcriptional profiling provides insights into the evolution and development of the zygomorphic flower of Vicia sativa (Papilionoideae). Plos One 8, e57338.CrossRefGoogle Scholar
Liu, ZP, Liu, P, Luo, D, Liu, WX and Wang, YR (2014) Exploiting lllumina sequencing for the development of 95 novel polymorphic EST – SSR markers in common vetch (Vicia sativa subsp. sativa). Molecules 19, 5777–5789.CrossRefGoogle Scholar
Liu, P, Ma L, C, Wang, YR and Liu, ZP (2015) Observation pollen morphology of 16 Vicia Accessions using scanning electron microscopy. Acta Prataculturae Sinica 24, 107–114.Google Scholar
LPWG (Lgume Phylogeny Working Group) (2017) A new subfamily classification of the Leguminosae based on a taxonomically comprehensive phylogeny. Taxon 66, 44–77.CrossRefGoogle Scholar
Maxted, N (1993) A phenetic investigation of Vicia L. subgenus Vicia (Leguminosae, Vicieae). Botanical Journal of the Linnean Society 111(2), 155–182.10.1006/bojl.1993.1013CrossRefGoogle Scholar
Maxted, N and Hawkes, JG (1997) Selection of target taxa. Plant Genetic Conservation: The in Situ Approach, 43–68.Google Scholar
Potokina, E, Tomooka, N, Vaughan, DA, Alexandrova, T and Qiang, XR (1999) Phylogeny of Vicia Subgenus Vicia (Fabaceae) based on analysis of RAPDs and RFLP of PCR-amplified chloroplast genes. Genetic Resources and Crop Evolution 46, 149–161.CrossRefGoogle Scholar
Punt, W, Hoen, PP, Blackmore, S and Nilssont, S (2007) Glossary of pollen and spore terminology. Review of Palaeobotany and Palynology 143, 1–81.CrossRefGoogle Scholar
Quamar, MF, Ali, SN, Pandita, SK and Singh, Y (2017) Modern pollen rain from Udhampur (Jammu and Kashmir), India: insights into pollen production, dispersal, transport and preservation. Palynology 42, 1–11.Google Scholar
Watson, SL, De Lacy, IH, Podlich, DW and Basford, KE (1995) GEBEIL: an analysis package using agglomerative hierarchical classificatory and SVD ordination procedures for genotype 9 environment data. Centre for Statistics Research Report, Department of Agriculture, University of Queensland, Brisbane, Australia.Google Scholar
White, TL and Hodge, GR (1989) Predicting Breeding Values with Applications in Forest Tree Improvement, vol. 33. Boston, MA: Kluwer Academic.CrossRefGoogle Scholar
Wodehouse, RP (1935) Pollen Grains: Their structure, identification and significance in science and medicine. Journal of Nervous & Mental Disease 86, 104.10.1097/00005053-193707000-00047CrossRefGoogle Scholar