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Protein synthesis in muscle of mature sheep

Published online by Cambridge University Press:  27 March 2009

D. T. W. Bryant  and
R. W. Smith
D. T. W. Bryant
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
R. W. Smith
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
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Summary

Protein synthesis was measured in wether sheep by constant intravenous infusion of [3H]tyrosine. The specific radioactivity of plasma tyrosine at plateau was used to calculate tyrosine flux and the rate of protein synthesis in the whole body was estimated. For wethers fed hay and concentrates tyrosine flux was 2·46 mmol/h and protein synthesized was 5·29 g/kg body weight per day. Corresponding values for wethers fed barley straw were 30–35% lower.

Fractional rates of protein synthesis in individual muscles were between 2 and 3% per day for diaphragm, longissimus dorsi, gastrocnemius, semitendinosus and vastus lateralis muscles from wethers fed hay and concentrates, but the value for vastus intermedius was higher. Corresponding rates for wethers fed barley straw were 18–36% lower.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 98 , Issue 3 , June 1982 , pp. 639 - 643
Copyright
Copyright © Cambridge University Press 1982

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