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The potential digestibility of cellulose in grasses and its relationship with chemical and anatomical parameters

Published online by Cambridge University Press:  27 March 2009

R. J. Wilkins
R. J. Wilkins
Affiliation:
Agronomy Department, University of New England, Armidale, Australia
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Summary

Potential cellulose digestibility, measured by incubation in vitrofor 6 days, decreased during floral development in perennial ryegrass, Wimmera ryegrass, cocksfoot, oat and tall fescue. The rate of decline was slower than for cellulose digestibility measured after incubation in vitro for 2 days only. Morphological fractions ranked in order of descending potential cellulose digestibility – leaf blade, inflorescence, leaf sheath and stem.

Lignin content was determined chemically by the method of Van Soest (1963) and lignified tissue was assessed by staining transverse sections of leaf blades and leaf sheaths with safranin and fast green. Both lignin and lignified tissue increased with maturity. Lignified tissue increased mainly through increase in the number of scleren-chyma cells, but was also affected by the formation of lacunae or cavities between the vascular bundles in leaf blades of cocksfoot and in leaf sheaths of all species studied. For 19 samples of leaf blades and leaf sheaths, potential cellulose digestibility had significant negative correlations with both lignin content (r = -0·862) and lignified tissue (r = -0·905). Limitations to the techniques used to assess lignification and further factors which may affect the relationship between lignification and potential cellulose digestibility are discussed.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 78 , Issue 3 , June 1972 , pp. 457 - 464
Copyright
Copyright © Cambridge University Press 1972

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References

REFERENCES

Armstrong, D. G., Blaxter, K. L. & Waite, R. (1964). The evaluation of artificially dried grass as a source of energy for sheep. III. The prediction of nutritive value from chemical and biological measurements. J. agric. Sci., Camb. 62, 417–24.CrossRefGoogle Scholar
Barnett, A. G. (1957). Studies on the digestibility of the cellulose fraction of grassland products. J. agric. Sci., Camb. 49, 467–74.CrossRefGoogle Scholar
Bondi, A. H. & Meyer, H. (1943). On the chemical nature and digestibility of roughage carbohydrates. J. agric. Sci., Camb. 33, 123–8.CrossRefGoogle Scholar
Bukdick, D. & Sullivan, J. T. (1963). Ease of hydrolysis of the hemicelluloses of forage plants in relation to digestibility. J. Anim. Sci. 22, 444–7.CrossRefGoogle Scholar
Connor, H. E. (1960). Variation in leaf anatomy in Festuca novae-zelandiae (Hack). Cockayne and Festuca mathewsii (Hack) Cheeseman. N. Z. Jl Sci. 3, 468–509.Google Scholar
Crampton, E. W. & Maynard, L. A. (1938). The relation of cellulose and lignin content to the nutritive value of animal feeds. J. Nutr. 15, 383–95.CrossRefGoogle Scholar
Deinum, B. & Van Soest, P. J. (1969). Prediction of forage digestibility from some laboratory procedures. Neth. J. agric. Sci. 17, 119–27.Google Scholar
Forbes, R. M. (1950). Protein as an indicator of pasture forage digestibility. J. Anim. Sci. 9, 231–7.CrossRefGoogle ScholarPubMed
Forbes, R. M. & Garrigus, W. P. (1950). Some effects of forage consumption on its nutritive value when cut and fed green to steers and wethers as determined conventionally and by lignin ratio. J. Anim. Sci. 9, 531–9.CrossRefGoogle Scholar
Gaillard, B. D. E. (1962). The relationship between cell wall constituents of roughage and the digestibility of the organic matter. J. agric. Sci., Camb. 59, 369–73.CrossRefGoogle Scholar
Hellstrom, N. & Aamisepp, M. (1965). Digestion. III. Faecal analyses and digestibility. J. Sci. Fd Agric. 16, 27–33.CrossRefGoogle Scholar
Jarrige, R. & Minson, D. J. (1964). Digestibility des constituents du raygrass anglais S. 24 et du dactyle S. 37, plus specialement des constituents glucidique. Annls Zootech. 13, 117–50.CrossRefGoogle Scholar
Johansen, D. A. (1940). Plant Micro-Technique. New York: McGraw-Hill.Google Scholar
Johnston, M. J. & Waite, R. (1965). Studies in the lignification of grasses. I. Perennial ryegrass (S. 24) and cocksfoot (S. 37). J. agric. Sci., Camb. 64, 211–19.CrossRefGoogle Scholar
Kaufman, P. B. (1959). Development of the shoot of Oryza sativa. III. Early stages in the ihistogenesis of the stem and ontogeny of the adventitious root. Phytomorphology 9, 382–404.Google Scholar
Kefford, N. P. (1958). Lignification of plants in relation to ruminant nutrition. J. Aust. Inst. agric. Sci. 24, 297–302.Google Scholar
Kneebone, W. R. (1962). A simple rapid precise staining procedure for identification of lignified tissue in grasses. Crop Sci. 2, 268.CrossRefGoogle Scholar
Lewton-Brain, L. (1903). On the anatomy of theleaves of British grasses. Trans. Linn. Soc. Lond. (2nd Ser., Botany) 6, 315–59.Google Scholar
McKenzie, H. A. & Wallace, H. S. (1954). The Kjeldahl determination of nitrogen: a critical study of digestion conditions-temperature, catalyst and oxidizing agent. Aust. J. Chem. 7, 55–70.CrossRefGoogle Scholar
Metcalfe, C. R. (1960). Anatomy of the Mono-Cotyledons. I. Qramineae. Oxford: Clarendon Press.Google Scholar
Osbourn, D. F., Terry, R. A., Outen, G. E., Cammell, S. B. & Lansley, P. R. (1971). Chemical and in vitro digestion procedures for the prediction of the digestibility of forage by sheep. Proc. Nutr. Soc. 31, 85A–86A.Google Scholar
Regal, V. (1960). The evaluation of quality of pasture grasses by the microscopic method. Proc. 8th Int. Orassld Congr., Reading, pp. 522–4.Google Scholar
Roelofsen, P. A. (1959). The plant cell-wall. Hand. Pfl Anat. 3, no. 4.Google Scholar
Stafford, H. A. (1962). Histochemical and biochemical differences between lignin-like materials in Phleum pratense L. Pl. Physiol., Lancaster 37, 643–9.CrossRefGoogle ScholarPubMed
Terry, R. A. & Tilley, J. M. A. (1964). The digestibility of the leaves and stems of perennial ryegrass, cocksfoot, timothy, tall fescue, lucerne and sainfoin as measured by an in vitro procedure. J. Br. Grassld Soc., 19, 363–72.CrossRefGoogle Scholar
Van Soest, P. J. (1963). Use of detergents in the analysis of fibrous feeds. II. A rapid method for the determination of fibre and lignin. J. Ass. off. agric. Chem. 46, 829–35.Google Scholar
Van Soest, P. J. (1964). Symposium on nutrition and forage and pastures: new chemical procedures for evaluating forages. J. Anim. Sci. 23, 838–45.CrossRefGoogle Scholar
Van Soest, P. J. (1967). Development of a comprehensive system of feed analysis and its application to forages. J. Anim. Sci. 26, 119–28.CrossRefGoogle Scholar
Van Soest, P. J. (1969). Chemical properties of fiber in concentrate feedstuffs. Proc. 1969 Cornell Nutrition Conference for Feed Manufacturers, pp. 17–21.Google Scholar
Van Soest, P. J. & Jones, L. H. P. (1968). Effect of silica in forages upon digestibility. J. Dairy Sci. 51, 1644–9.CrossRefGoogle Scholar
Waite, R., Johnston, M. J. & Armstrong, D. G. (1962). The digestibility of the carbohydrate constituents of S. 23 ryegrass cut at four stages of growth. Proc. Nutr. Soc. 21, xxi–xxii.Google Scholar
Waite, R., Johnston, M. J. & Armstrong, D. G. (1964). The evaluation of artificially dried grass as a source of energy for sheep. I. The effect of stage of maturity on the apparent digestibility of ryegrass, cocksfoot and timothy. J. agric. Sci., Camb. 62, 192–7.CrossRefGoogle Scholar
Walker, D. M. & Hepburn, W. R. (1955). The analysis of roughage by the normal acid fibre method and its use for predicting the digestibility of roughages by sheep. Agric. Prog. 30, 118–19.Google Scholar
Whitehead, D. L. & Quicke, G. V. (1964). A comparison of six methods of estimating lignin in grass hay. J. Sci. Fd Agric. 15, 417–22.CrossRefGoogle Scholar
Wilkins, R. J. (1966). The digestibility of forage and its relationship to lignification. Ph.D. Thesis. University of New England.Google Scholar
Wilkins, R. J. (1969). The potential digestibility of cellulose in forage and faeces. J. agric. Sci., Camb. 73, 57–64.CrossRefGoogle Scholar
Wilkins, R. J. (1972). The in vitro digestion of grass stems. Paper submitted to J. agric. Sci., Camb.Google Scholar