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Phosphorus affects high-molecular-weight glutenin subunits and glutenin macropolymer size distribution in wheat grains

Published online by Cambridge University Press:  23 July 2013

Y. NI ,
D. YANG ,
Z. WANG ,
Y. YIN ,
T. CAI ,
Z. DAI ,
S. YAN  and
W. LI
Y. NI
Affiliation:
National Key Laboratory of Crop Biology, Agronomy College of Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China Agricultural Bureau of Rencheng District, Jining City of Shandong Province, Jining 272100, Shandong, People's Republic of China
D. YANG
Affiliation:
National Key Laboratory of Crop Biology, Agronomy College of Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China
Z. WANG*
Affiliation:
National Key Laboratory of Crop Biology, Agronomy College of Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China
Y. YIN
Affiliation:
National Key Laboratory of Crop Biology, Agronomy College of Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China
T. CAI
Affiliation:
National Key Laboratory of Crop Biology, Agronomy College of Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China
Z. DAI
Affiliation:
Biology Department, Dezhou University, Dezhou 253023, Shandong, People's Republic of China
S. YAN
Affiliation:
College of Plant Science, Anhui Science and Technology University, Fengyang 233100, Anhui, People's Republic of China
W. LI
Affiliation:
College of Plant Science, Anhui Science and Technology University, Fengyang 233100, Anhui, People's Republic of China
*
*To whom all correspondence should be addressed. Email: [email protected]
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Summary

Two winter wheat (Triticum aestivum L.) cultivars, Jimai20 and Shannong12, differing in phosphorus (P) utilization efficiency, were selected to study the effect of P application rate on changes in glutenin macropolymer (GMP) size distribution and the content of high-molecular-weight glutenin subunits (HMW-GS) in wheat grain. Four P levels (0, 40, 100 and 160 kg/ha) were applied under N1 (112·5 kg/ha) and N2 (225 kg/ha) conditions in the field, in 2008/09 and 2009/10. The results showed that increased P levels favoured HMW-GS synthesis under N1 conditions, but had a less pronounced effect under N2. When compared with the control, the volume proportions of <10 μm GMP particles in grains of both cultivars were significantly decreased, and those of >100 μm were increased in response to P application. The volume proportions of 10–100 μm GMP particles in the cultivars Jimai20 and Shannong12 were respectively lower and higher in response to P application than with no P fertilizer. At maturity, for both cultivars, total HMW-GS content was negatively correlated with GMP particle volume of <10 μm, but positively correlated with that of >100 μm. These observations suggest that both P and N affect protein synthesis in wheat grains and there exists a relationship between HMW-GS content and the synthesis of large GMP particles (>100 μm). The N×P interaction was the most important factor to regulate the HMW-GS and GMP contents.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 152 , Issue 5 , October 2014 , pp. 759 - 769
Copyright
Copyright © Cambridge University Press 2013 

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