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Measurements of the rate of production of bacteria in the rumen of buffalo calves

Published online by Cambridge University Press:  27 March 2009

U. B. Singh
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (Uttar Pradesh), India
D. N. Verma
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (Uttar Pradesh), India
A. Varma
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (Uttar Pradesh), India
S. K. Ranjhan
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (Uttar Pradesh), India

Summary

A technique is described for the in vivo estimation of the rate of production of bacteria in the rumen of buffalo calves. The animals were given their daily ration in 12 equal amounts at 2-h intervals. The bacterial cells from the rumen were labelled either with 14C or 36S by in vitro incubation in the presence of [U-14C]DL-leucine or 35S-sodium sulphate. Labelled bacterial cells were injected in a single dose into the rumen. Samples of the ruminal fluid were drawn at various time intervals for 9 h and the specific radio-activity of the bacteria determined. The dilution in the specific radioactivity was used to calculate the turn-over time and rates of production of bacteria in the rumen. The average turnover time was 308 min. The production rate of bacteria averaged 211 mg/min (20·3 g/mole VFA produced).

Type
Research Article
Copyright
Copyright © Cambridge University Press 1974

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References

REFERENCES

A.O.A.C. (1960). Official Methods of Analysis (9th ed.), Washington, D.C. Association of Official Agricultural Chemists.Google Scholar
Baldwin, R. L., Lucas, H. L. & Cabrera, R. (1970). In Physiology of Digestion and Metabolism in the Ruminant (ed. Phillipson, A. T.), p. 319. Newcastleupon-Tyne, England: Oriel Press.Google Scholar
Chaturvedi, M. L., Singh, U. B. & Ranjhan, S. K. (1973). Measurements of entry rates of volatile fatty acids in Murrah buffaloes by isotope dilution, using single injection technique. Indian Journal of Animal Production (in the Press).Google Scholar
Forrest, W. W. & Walker, D. J. (1971). In Advances in Microbial Physiology (ed. Wilkinson, J. N. and Rose, A. H.), p. 213. New York: Academic Press.Google Scholar
Harrison, D. G., Beever, D. E. & Thomson, D. J. (1972). Estimation of food and microbial protein in duodenal digesta. Proceedings of the Nutrition Society 31, 60 A.Google ScholarPubMed
Henderickx, H. K. (1959). The in vitro investigations of protein synthesis in the rumen of ruminants by 35S. VI. Tijdschrift voor diergeneeskunde 28, 80.Google Scholar
Henderickx, H. K. (1961). Incorporation of sulphate in the ruminal proteins. Archives of International Physiological Biochemistry 69, 449.CrossRefGoogle ScholarPubMed
Hoogenraad, N. J., Hird, F. J. R., White, R. G. & Leng, R. A. (1970). Utilization of 14C-labelled Bacillus subtilis and Escherichia coli by sheep. British Journal of Nutrition 24, 129.CrossRefGoogle ScholarPubMed
Hungate, R. E. (1966). In The Rumen and its Microbes, p. 319. New York: Academic Press.Google Scholar
Leng, R. A. (1972). Salient features of the digestion of pastures by ruminants and other herbivores. In Chemistry and Biochemistry of Herbage (ed. Bailey, R. W. and Butler, G. W.). New York: Academic Press.Google Scholar
Mathison, G. W. & Milligan, L. P. (1971). Nitrogen metabolism in sheep. British Journal of Nutrition 25, 351.CrossRefGoogle ScholarPubMed
McDougall, E. I. (1948). Studies on ruminant saliva. 1. The comparison and output of sheep's saliva. Biochemical Journal 43, 99.CrossRefGoogle Scholar
Moustafa, H. H. & Collins, E. B. (1968). Molar growth yields of certain lactic acid bacteria as influenced by autolysis. Journal of Bacteriology 96, 117.CrossRefGoogle ScholarPubMed
Roberts, S. A. & Miller, E. L. (1969). An estimate of bacterial protein synthesis in sheep on a constant feeding regime. Proceedings of the Nutrition Society 28, 32 A.Google ScholarPubMed
Singh, U. B., Chaturvedi, M. L., Varma, A. & Ranjhan, S. K. (1973). Determination of entry rates of volatile fatty acids in Bos bubalis and Bos indicus by isotope dilution using continuous infusion technique. Indian Journal of Animal Production 4, 37.Google Scholar
Singh, U. B., Varma, A., Verma, D. N., Lal, M. & Ranjhan, S. K. (1973). In vivo measurements of the production rates of bacteria in the rumen. Journal of Agricultural Science, Cambridge 81, 349.CrossRefGoogle Scholar
Singh, U. B., Verma, D. N., Varma, A. & Ranjhan, S. K. (1973). Measurements of the rate of production of protozoa with the help of 35S in the rumen of cattle and buffalo. Indian Journal of Animal Science (in the Press).Google Scholar
Walker, D. J. & Nadar, C. J. (1968). Method for measuring microbial growth in rumen content. Applied Microbiology 16.CrossRefGoogle ScholarPubMed