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Isolation of anatomically defined cell walls from fodder kale, and their contributions to determining the in vitro cellulase digestibility of the whole plant

Published online by Cambridge University Press:  27 March 2009

J. G. McCluskey ,
M. J. Allison ,
H. J. Duncan  and
M. C. Jarvis
J. G. McCluskey
Affiliation:
Scottish Crop Research Institute, Pentlandfield, Midlothian
M. J. Allison
Affiliation:
Scottish Crop Research Institute, Pentlandfield, Midlothian
H. J. Duncan
Affiliation:
Agricultural Chemistry, Glasgow University, Glasgow, G12 8QQ
M. C. Jarvis
Affiliation:
Agricultural Chemistry, Glasgow University, Glasgow, G12 8QQ
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Summary

Vascular and non-vascular cell walls were isolated separately from leaves, upper stems and lower stems of 12 kale (Brassica oleracea L.) cultivars, by a sieving technique. The digestible organic matter in the dry matter (DOMD) of the cell walls and of the whole plants was determined by pepsin-cellulase digestion. The measured whole-plant DOMD correlated closely with the DOMD predicted by adding together the amounts of non-digested material derived from all the plant's component cell-wall fractions. Differences in DOMD between cultivars were determined primarily by the amount of vascular cell walls in the stems, particularly the lower stems; that is, by the stem anatomy. The vascular cell walls of the upper stems had a wider range of DOMD values and a higher mean DOMD than the vascular cell walls of the lower stems. Thus cell-wall composition made some contribution to determining the whole-plant DOMD, although it contributed less than the anatomy of the stem.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 103 , Issue 2 , October 1984 , pp. 347 - 352
Copyright
Copyright © Cambridge University Press 1984

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References

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