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Influence of sucrose supplementation on nitrogen kinetics and energy metabolism in sheep fed with lucerne hay cubes

Published online by Cambridge University Press:  27 March 2009

Y. Obara ,
H. Fuse ,
F. Terada ,
M. Shibata ,
A. Kawabata ,
M. Sutoh ,
K. Hodate  and
M. Matsumoto
Y. Obara
Affiliation:
National Institute of Animal Industry, Tsukuba Norindanchi, PO Box 5, Ibaraki 305, Japan
H. Fuse
Affiliation:
National Institute of Animal Industry, Tsukuba Norindanchi, PO Box 5, Ibaraki 305, Japan
F. Terada
Affiliation:
National Institute of Animal Industry, Tsukuba Norindanchi, PO Box 5, Ibaraki 305, Japan
M. Shibata
Affiliation:
National Institute of Animal Industry, Tsukuba Norindanchi, PO Box 5, Ibaraki 305, Japan
A. Kawabata
Affiliation:
National Institute of Animal Industry, Tsukuba Norindanchi, PO Box 5, Ibaraki 305, Japan
M. Sutoh
Affiliation:
National Institute of Animal Industry, Tsukuba Norindanchi, PO Box 5, Ibaraki 305, Japan
K. Hodate
Affiliation:
National Institute of Animal Industry, Tsukuba Norindanchi, PO Box 5, Ibaraki 305, Japan
M. Matsumoto
Affiliation:
National Institute of Animal Industry, Tsukuba Norindanchi, PO Box 5, Ibaraki 305, Japan
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Summary

The effects of sucrose supplementation on nitrogen kinetics and energy metabolism were examined in sheep fed lucerne hay cubes using a 15N isotope dilution method and balance and respiration trials in Tsukuba, Japan in 1988. Sheep were fed lucerne hay cubes (1183 g DM/day), with or without 204 g/day sucrose, at 2 h intervals from continuous feeders. Supplementation with sucrose decreased urinary N excretion (P < 0·01), resulting in an increase in N retention from – 1·1 to + 2·0 g N/day (P < 0·01). Supplementation with sucrose resulted in no change in faeces and methane energy, a decrease in urinary energy (P < 0·01) and an increase in heat production and energy balance (P < 0·01). Sucrose supplementation also resulted in lower rumen ammonia (P < 0·05) and plasma urea concentrations (P < 0·05) and reduced urinary urea excretion (P < 0·01). The fermentation of sucrose in the rumen resulted in a decrease in rumen pH (P < 0·01) and in the acetate: propionate ratio (P < 0·05). Sucrose supplementation increased the proportion of urea transferred to the rumen (P < 0·05), non-ammonia N (NAN) concentration in the rumen (P < 0·001) and NAN flow from the rumen to the lower digestive tract (P < 0·001). Urinary allantoin excretion rate increased with sucrose supplementation (P < 0·05). The plasma glucose concentration was unchanged but plasma insulin concentration was increased with sucrose supplementation (P < 0·05). The influence of energy-rich supplements, such as sucrose, on N kinetics and the mechanism of the increase in N retention with sucrose supplementation are discussed.

Type
Animals
Information
The Journal of Agricultural Science , Volume 123 , Issue 1 , August 1994 , pp. 121 - 127
Copyright
Copyright © Cambridge University Press 1994

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References

Abe, A., Horii, S. & Kameoka, K. (1979). Application of enzymatic analysis with glucoamylase, pronase and cellulase to various feeds for cattle. Journal of Animal Science 48, 1483–1490.Google Scholar
Broderick, G. A. & Wallace, R. J. (1988). Effects of dietary nitrogen source on concentrations of ammonia, free amino acids and fluorescamine-reactive peptides in the sheep rumen. Journal of Animal Science 66, 2233–2238.CrossRefGoogle Scholar
Burroughs, W., Gerlaugh, P., Edgington, B. H. & Bethke, R. M. (1949). The influence of corn starch upon roughage digestion in cattle. Journal of Animal Science 8, 271–278.Google Scholar
Chappell, G. L. M. & Fontenot, J. P. (1968). Effect of level of readily-available carbohydrates in purified sheep rations on cellulose digestibility and nitrogen utilization. Journal of Animal Science 27, 1709–1715.CrossRefGoogle Scholar
Chen, X. B., Hovell, F. D. DeB., Ørskov, E. R. & Brown, D. S. (1990). Excretion ofpurinederivatives by ruminants: effect of exogenous nucleic acid supply on purine derivative excretion by sheep. British Journal of Nutrition 63, 131–142.Google Scholar
Coulombe, J. J. & Favreav, L. (1963). A new simple semimicro method for colorimetric determination of urea. Clinical Chemistry 9, 102–108.Google Scholar
Dellow, D. W., Obara, Y., Kelly, K. E. & Sinclair, B. R. (1988). Improving the efficiency of utilisation of pasture protein by sheep. Proceedings of the New Zealand Society of Animal Production 48, 253–255.Google Scholar
Fujihara, T., Ørskov, E. R., Reeds, P. J. & Kyle, D. J. (1987). The effect of protein infusion on urinary excretion of purine derivatives in ruminants nourished by intragastric nutrition. Journal of Agricultural Science, Cambridge 109, 7–12.Google Scholar
Kennedy, P. M. (1980). The effects of dietary sucrose and the concentrations of plasma urea and rumen ammonia on the degradation of urea in the gastrointestinal tract of cattle. British Journal of Nutrition 43, 125–140.CrossRefGoogle ScholarPubMed
Kennedy, P. M., Clarke, R. T. J. & Milligan, L. P. (1981). Influences of dietary sucrose and urea on transfer of endogenous urea to the rumen of sheep and numbers of epithelial bacteria. British Journal of Nutrition 46, 533–541.Google Scholar
Mathers, J. C. & Miller, E. L. (1981). Quantitative studies of food protein degradation and the energetic efficiency of microbial protein synthesis in the rumen of sheep given chopped lucerne and rolled barley. British Journal of Nutrition 45, 587–604.Google Scholar
McAllan, A. B. (1982). The fate of nucleic acids in ruminants. Proceedings of the Nutrition Society 41, 309–317.Google Scholar
McAllan, A. B. & Smith, R. H. (1973). Degradation of nucleic acid derivatives by rumen bacteria in vitro. British Journal of Nutrition 29, 467–474.Google Scholar
McGinn, R. & McLellan, A. R. (1986). Energy metabolism in lambs fed silage diets with sucrose or a growth promoter. Animal Feed Science and Technology 16, 203–213.Google Scholar
Mould, F. L., Ørskov, E. R. & Mann, S. O. (1983). Associative effects of mixed feeds. I. Effects of type and level of supplementation and the influence of the rumen fluid pH on cellulolysis in vivo and dry matter digestion of various roughages. Animal Feed Science and Technology 10, 15–30.CrossRefGoogle Scholar
Nolan, J. V. & Leng, R. A. (1972). Dynamic aspects of ammonia and urea metabolism in sheep. British Journal of Nutrition 27, 177–194.Google Scholar
Nolan, J. V. & Leng, R. A. (1974). Isotope techniques for studying the dynamics of nitrogen metabolism in ruminants. Proceedings of the Nutrition Society 33, 1–8.Google Scholar
Nolan, J. V., Norton, B. W. & Leng, R. A. (1976). Further studies of the dynamics of nitrogen metabolism in sheep. British Journal of Nutrition 35, 127–147.Google Scholar
Obara, Y. & Dellow, D. W. (1993). Effects of intraruminal infusion of urea, sucrose or urea plus sucrose on plasma urea and glucose kinetics in sheep fed chopped lucerne hay. Journal of Agricultural Science, Cambridge 121, 125–130.Google Scholar
Obara, Y., Dellow, D. W. & Nolan, J. V. (1991). The influence of energy-rich supplements on nitrogen kinetics in ruminants. In Physiological Aspects of Digestion and Metabolism in Ruminants (Eds Tsuda, T., Sasaki, Y. & Kawashima, R.), pp. 515–539. San Diego: Academic Press.CrossRefGoogle Scholar
Sas Institute (1989). SAS/STAT User's Guide, Version 6, 4th edn., Vol 2. Cary, NC: SAS Institute Inc.Google Scholar
Shimbayashi, K., Obara, Y., Yonemura, T., Deguchi, N. & Nakanishi, M. (1973). Effect of capylohydroxamic acid on ruminal urease. Japanese Journal of Veterinary Science 35, 327–334Google Scholar
Sutoh, M., Obara, Y. & Miyamoto, S. (1991). The effect of feeding frequency and dietary sucrose on rumen fermentation, plasma metabolites and insulin in sheep. Animal Science and Technology 62, 1120–1128.Google Scholar
Sutoh, M., Obara, Y. & Yoneyama, T. (1993). The effects of feeding regimen and dietary sucrose supplementation on natural abundance of 15N in some components of ruminal fluid and plasma of sheep. Journal of Animal Science 71, 226–231.Google Scholar
Syrjala, L. (1972). Effect of different sucrose, starch and cellulose supplements on the utilization of grass silages by ruminants. Annales Agriculture Fenniae 11, 199–276.Google Scholar
Udén, P., Colucci, P. E. & Van Soest, P. J. (1980). Investigation of chromium, cerium and cobalt as markers in digesta. Rate of passage studies. Journal of the Science of Food and Agriculture 31, 625–632.Google Scholar
Weatherburn, M. W. (1967). Phenol-hypochloride reaction for determination of ammonia. Analytical Chemistry 39, 971–974.Google Scholar
Young, E. G. & Conway, C. F. (1942). On the estimation of allantoin by the Rimini-Schryver reaction. Journal of Biological Chemistry 144, 839–853.Google Scholar