Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-05T00:51:31.824Z Has data issue: false hasContentIssue false
  • English
  • Français

Incorporation of abomasal and intravenous doses of [35S]cystine and [35S]methionine into wool

Published online by Cambridge University Press:  27 March 2009

P. J. Reis ,
D. A. Tunks  and
L. F. Sharry
P. J. Reis
Affiliation:
CSIRO, Division of Animal Production, Ian Clunies Ross Animal Research LaboratoryP.O. Box 239BlacktownNSW 2148Australia
D. A. Tunks
Affiliation:
CSIRO, Division of Animal Production, Ian Clunies Ross Animal Research LaboratoryP.O. Box 239BlacktownNSW 2148Australia
L. F. Sharry
Affiliation:
CSIRO, Division of Animal Production, Ian Clunies Ross Animal Research LaboratoryP.O. Box 239BlacktownNSW 2148Australia
Get access Rights & Permissions [Opens in a new window]

Summary

The fate of abomasal and intravenous doses of [35S]cystine and [35S]methionine was investigated in sheep to determine their relative efficacy as supplements for wool growth.

Administration of L-[35S]cystine and L-, DL- and D-[35S]methionine via the abomasum produced similar patterns of 35S labelling in blood plasma. Only 10–16% of the administered 35S was excreted in urine over 7 days and 22–32% of the dose was incorporated into wool over 21 days. All isomers were utilized by the sheep with similar efficiency and it was concluded that D- and L-methionine and L-cystine are equally effective as supplements for wool growth at optimal dose rates. Abomasal supplementation with a large amount of methionine (10 g/day) resulted in the excretion of a high proportion of the 35S in urine (55% of the dose over 7 days) and low incorporation into wool.

A comparison of the extent of incorporation of 35S into wool from intravenous doses of L-[35S]cystine and L-[35S]methionine in the same sheep, and in sheep from flocks selected for high and low wool production, showed that incorporation from methionine averaged 80% of that from cystine. It was concluded that, at moderate (2–3 g/day) methionine availability, methionine is utilized for synthesis of wool with about 80% of the efficiency of cystine, conversion of methionine to cysteine occurring via the transulphuration pathway.

Type
Review
Information
The Journal of Agricultural Science , Volume 112 , Issue 3 , June 1989 , pp. 313 - 319
Copyright
Copyright © Cambridge University Press 1989

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ahmed, W., Dun, R. B. & Winston, R. J. (1963). The efficiency of conversion of feed to wool in Merino flocks selected for and against fleece weight. Australian Journal of Experimental Agriculture and Animal Husbandry 3, 269–275.CrossRefGoogle Scholar
Benevenga, N. J., Radcliffe, B. C. & Egan, A. R. (1983). Tissue metabolism of methionine in sheep. Australian Journal of Biological Sciences 36, 475–485.CrossRefGoogle ScholarPubMed
Broad, A., Gillespie, J. M. & Reis, P. J. (1970). The influence of sulphur-containing amino acids on the biosynthesis of high-sulphur wool proteins. Australian Journal of Biological Sciences 23, 149–164.CrossRefGoogle Scholar
Coelho Da Silva, J. F., Seeley, R. C., Thomson, D. J., Beever, D. E. & Armstrong, D. G. (1972). The effect in sheep of physical form on the sites of digestion of a dried lucerne diet. 2. Sites of nitrogen digestion. British Journal of Nutrition 28, 43–61.CrossRefGoogle ScholarPubMed
Downes, A. M. (1961). The fate of intravenous doses of free and plasma protein-bound [35S]cystine in the sheep. Australian Journal of Biological Sciences 14, 427–439.CrossRefGoogle Scholar
Downes, A. M., Reis, P. J., Sharry, L. F. & Tunks, D. A. (1970 a). Metabolic fate of parenterally administered sulphur-containing amino acids in sheep and effects on growth and composition of wool. Australian Journal of Biological Sciences 23, 1077–1088.Google ScholarPubMed
Downes, A. M., Reis, P. J., Sharry, L. F. & Tunks, D. A. (1970 b). Evaluation of modified [35S]methionine and [35S]casein preparations as supplements for sheep. British Journal of Nutrition 24, 1083–1089.CrossRefGoogle ScholarPubMed
Downes, A. M., Sharry, L. F. & Till, A. R. (1964). The fate of intradermal doses of labelled amino acids in sheep. Australian Journal of Biological Sciences 17, 945–959.CrossRefGoogle Scholar
Doyle, P. T. (1981). Sulfur and methionine metabolism in sheep. V. Utilization of methionine isomers. Australian Journal of Biological Sciences 34, 47–59.CrossRefGoogle ScholarPubMed
Dun, R. B. (1958). The influence of selection and plane of nutrition on the components of fleece weight in Merino sheep. Australian Journal of Agricultural Research 9, 802–818.CrossRefGoogle Scholar
du Vigneaud, V. (1952). A Trail of Research in Sulfur Chemistry and Metabolism and Related Fields. Ithaca: Cornell University Press.Google Scholar
Egan, A. R., Benevenga, N. J. & Radcliffe, B. C. (1984). The metabolism of S-amino acids in sheep. In Ruminant Physiology: Concepts and Consequences (ed. Baker, S. K., Gawthome, J. M., Mackintosh, J. B. and Purser, D. B.), pp. 241–252. Perth: University of Western Australia.Google Scholar
Finkelstein, J. D. (1970). Control of sulfur metabolism in mammals. In Symposium: Sulfur in Nutrition (ed. Muth, O. H. and Oldfield, J. E.), pp. 46–60. Westport: Avi Publishing Co.Google Scholar
Finkelstein, J. D. & Mudd, S. H. (1967). Trans-sulfuration in mammals: the methionine-sparing effect of cystine. Journal of Biological Chemistry 242, 873–880.CrossRefGoogle ScholarPubMed
Gillespie, J. M. (1983). The structural proteins of hair: isolation, characterization and regulation of biosynthesis. In Biochemistry and Physiology of the Skin (ed. Goldsmith, L. A.), pp. 475–510. New York: Oxford University Press.Google Scholar
Pisulewski, P. M. & Buttery, P. J. (1985). The effect of increasing methionine supply on the methionine conversion to cyst(e)ine in sheep. British Journal of Nutrition 54, 121–129.CrossRefGoogle ScholarPubMed
Reis, P. J. (1967). The growth and composition of wool. IV. The differential response of growth and of sulphur content of wool to the level of sulphur-containing amino acids given per abomasum. Australian Journal of Biological Sciences 20, 809–825.CrossRefGoogle Scholar
Reis, P. J. (1979). Effects of amino acids on the growth and properties of wool. In Physiological and Environmental Limitations to Wool Growth (ed. Black, J. L. and Reis, P. J.), pp. 223–242. Armidale: University of New England Publishing Unit.Google Scholar
Reis, P. J. & Schinckel, P. G. (1963). Some effects of sulphur-containing amino acids on the growth and composition of wool. Australian Journal of Biological Sciences 16, 218–230.CrossRefGoogle Scholar