Hostname: page-component-848d4c4894-8bljj Total loading time: 0 Render date: 2024-07-05T14:48:27.208Z Has data issue: false hasContentIssue false
  • English
  • Français

In vivo measurements of the production rates of bacteria in the rumen

Published online by Cambridge University Press:  27 March 2009

U. B. Singh ,
A. Varma ,
D. N. Verma ,
M. Lal  and
S. K. Ranjhan
U. B. Singh
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (U.P.), India
A. Varma
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (U.P.), India
D. N. Verma
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (U.P.), India
M. Lal
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (U.P.), India
S. K. Ranjhan
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (U.P.), India
Get access Rights & Permissions [Opens in a new window]

Summary

The production rates of bacteria have been measured in the rumen of zebu calves and buffalo calves. The animals were fed green oat continuously at 2 h intervals. [35S]sodium sulphate was fed to the animals for 5 days at 2 h intervals by mixing in the feed. On the sixth day the radioactive feed was stopped and replaced by non-radioactive feed. The decline in the specific radioactivity of bacterial cells in the rumen as a function of time was taken for calculating t/2. Simultaneously rumen volume was determined and pool size of bacterial cells was calculated in the rumen. A mathematical equation was applied to calculate the production rates of bacteria. The production rates of bacteria, on average, were 100·5 and 72·3 mg/min for buffalo calves and zebu calves, respectively.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 81 , Issue 2 , October 1973 , pp. 349 - 351
Copyright
Copyright © Cambridge University Press 1973

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Chaturvedi, M. L., Singh, U. B. & Ranjhan, S. K. (1973 a). Effect of feeding water soaked and dry wheat straw on feed intake, digestibility of nutrients and VFA production in growing zebu and buffalo calves. J. agric. Sci., Gamb. 80, 393–7.CrossRefGoogle Scholar
Chaturvedi, M. L., Singh, U. B. & Banjhan, S. K. (1973 b). A note on correlations between the entry rates of volatile fatty acids and digestible organic matter intake in buffalo calves. Indian J. Anim. Sci. 43 (in the Press).Google Scholar
Henderickx, H. K. (1959). The in vitro investigations of protein synthesis in the rumen of ruminants by 35S. VI. Diergen. Tijdschr. 28, 80.Google Scholar
Henderickx, H. K. (1961). Incorporation of sulphate in the ruminal proteins. Archs. int. Physiol. Biochim. 69, 449.Google ScholarPubMed
I.A.E.A. (1970). Isotope techniques for studying animal protein production, from non-protein nitrogen. Tech. Rep. Series Int. atom. Energy Ag. no. 111. Vienna.Google Scholar
Singh, U. B., Verma, D. N., Varma, A. & Ranjhan, S. K. (1973). Measurements of protozoa production rates in the rumen of cattle and buffalo with the help of 35S. Indian J. Anim. Sci. (in the Press).Google Scholar
Smith, R. H. (1959). The development and function of the rumen with milk fed calves. J. agric. Sci., Camb. 52, 72–8.CrossRefGoogle Scholar
Walker, D. J. & Nader, C. J. (1968). Methods of measuring mierobial growth in rumen contents. Appl. Microbiol. 16, 1124.CrossRefGoogle Scholar