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In vitro hydrolysis of urea-formaldehyde complexes made with or without molasses in the presence of phosphoric acid
Published online by Cambridge University Press: 27 March 2009
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The high activity of rumen urease is a major limiting factor in the usefulness of urea as a protein replacement supplement in ruminant diets (Bloomfield, Garner & Muhrer, 1960). The efficiency of urea utilization can be increased by decreasing the activity of rumen urease or by modification of urea in the products which are slow ammoniareleasing (Helmer & Bartley, 1971). An arbitrary formaldehyde treatment of urea was observed to lower the hydrolysis in the intact rumen (Pal & Negi, 1977). In a systematic study carried out in our laboratory a series of urea formaldehyde complexes (UFC) was prepared and their in vitro hydrolysis studied (Lall et al.1980; Makkar et al. 1981). Phosphoric acid is known to catalyse the reaction between aldehyde and urea. In the present study the complexes of urea and formaldehyde (UFC) and urea, formaldehyde and molasses (UFMC) in the presence of a catalyst (phosphoric acid) were prepared and their in vitro ammonia production is presented.
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