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Improvement of Cercospora leaf spot and powdery mildew resistance of mungbean variety KING through marker-assisted selection

Published online by Cambridge University Press:  12 January 2022

P. Papan ,
W. Chueakhunthod ,
W. Jinagool ,
A. Tharapreuksapong ,
A. Masari ,
C. Kaewkasi ,
S. Ngampongsai ,
T. Girdthai  and
P. A. Tantasawat Open the ORCID record for P. A. Tantasawat [Opens in a new window]
P. Papan
Affiliation:
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
W. Chueakhunthod
Affiliation:
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
W. Jinagool
Affiliation:
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
A. Tharapreuksapong
Affiliation:
Center for Scientific and Technological Equipment, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
A. Masari
Affiliation:
Field and Renewable Energy Crops Research Institute, 50 Phahonyothin Avenue, Chatuchak District, Bangkok 10900, Thailand
C. Kaewkasi
Affiliation:
School of Computer Engineering, Institute of Engineering, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
S. Ngampongsai
Affiliation:
Field and Renewable Energy Crops Research Institute, 50 Phahonyothin Avenue, Chatuchak District, Bangkok 10900, Thailand
T. Girdthai
Affiliation:
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
P. A. Tantasawat*
Affiliation:
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
*
Author for correspondence: P. A. Tantasawat, E-mail: [email protected]
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Abstract

The development of resistant mungbean varieties is one of the most efficient strategies to control major diseases such as Cercospora leaf spot (CLS) and powdery mildew (PM). The objectives of this study were to pyramid a CLS resistance gene and two PM resistance genes from the donor parent D2 into a susceptible variety KING through marker-assisted backcrossing (MABC) and to evaluate their agronomic traits and disease resistance under field conditions. Five markers linked to the resistance genes were used for foreground selection, while two marker sets [Set A containing 15 polymorphic simple sequence repeat (SSR) and expressed sequence tag-SSR (EST-SSR) markers and Set B containing 34 polymorphic inter-simple sequence repeat (ISSR) loci] were also used for background selection. Two pyramided backcross (BC) lines, namely H3 and H4, were homozygous at all five marker loci when confirmed in BC4F4 and BC4F5 generations. Their recurrent parent genome (RPG) recovery ranged from 96.4 to 100.0%, depending on the marker sets. During field evaluation, a moderate to high level of CLS and PM resistance was observed in both BC lines compared to the susceptible recurrent parent KING. One of these BC lines (H3) had all agronomic traits similar or superior to the recurrent parent KING at all environments, and had a higher yield than KING (18.0–32.0%) under CLS and PM outbreaks. This line can be developed into a new resistant mungbean variety in Thailand in the future. These results substantiate the usefulness of MABC for transferring multiple resistance genes into an elite variety.

Keywords

Field evaluationgene pyramidingrecurrent parent genome recoveryresistance geneVigna radiata (L.) Wilczek
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 159 , Issue 9-10 , November 2021 , pp. 676 - 687
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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References

Ahmed, F, Rafii, MY, Ismail, MR, Juraimi, AS, Rahim, HA, Tanweer, FA and Latif, MA (2016) Recurrent parent genome recovery in different populations with the introgression of Sub1 gene from a cross between MR219 and Swarna-Sub1. Euphytica 207, 605–618.CrossRefGoogle Scholar
Arsakit, K, Papan, P, Tharapreuksapong, A and Tantasawat, PA (2017) Simple sequence repeat markers associated with Cercospora leaf spot and powdery mildew resistance in mungbean (Vigna radiata L. Wilczek). Proceedings of the 2017 International Forum-Agriculture, Biology, and Life Science, Kyoto, Japan, pp. 7–15.Google Scholar
Arunakumari, K, Durgarani, CV, Satturu, V, Sarikonda, KR, Chittoor, PDR, Vutukuri, B, Laha, GS, Nelli, APK, Gattu, S, Jamal, M, Prasadbabu, A, Hajira, S and Sundaram, RM (2016) Marker-assisted pyramiding of genes conferring resistance against bacterial blight and blast diseases into Indian rice variety MTU1010. Rice Science 23, 306–316.CrossRefGoogle Scholar
AVRDC (1984) AVRDC Progress Report. Shanhua, Taiwan: Asian Vegetable Research and Development Center.Google Scholar
Baliyan, N, Malik, R, Rani, R, Mehta, K, Vashisth, U, Dhillon, S and Boora, KS (2018) Integrating marker-assisted background analysis with foreground selection for pyramiding bacterial blight resistance genes into Basmati rice. Comptes Rendus Biologies 341, 1–8.CrossRefGoogle ScholarPubMed
Carneiro, FF, dos Santos, JB and Leite, ME (2010) Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common bean. Electronic Journal of Biotechnology 13, 1–8.Google Scholar
Chai Nat Field Crops Research Center (2018) Guidelines for Data Record in Mungbean Research. Chai Nat, Thailand: Chai Nat Field Crops Research Center, Field and Renewable Energy Crops Research Institute, Department of Agriculture.Google Scholar
Chand, R, Singh, V, Pal, C, Kumar, P and Kumar, M (2012) First report of a new pathogenic variant of Cercospora canescens on mungbean (Vigna radiata) from India. New Disease Report 26, 6.CrossRefGoogle Scholar
Chand, R, Pal, C, Singh, V, Kumar, M, Singh, VK and Chowdappa, P (2015) Draft genome sequence of Cercospora canescens: a leaf spot causing pathogen. Current Science 109, 2103–2110.CrossRefGoogle Scholar
Chankaew, S, Somta, P, Sorajjapinun, W and Srinives, P (2011) Quantitative trait loci mapping of Cercospora leaf spot resistance in mungbean, Vigna radiata (L.) Wilczek. Molecular Breeding 28, 255–264.CrossRefGoogle Scholar
Chen, H, Wang, L, Wang, S, Liu, C, Blair, MW and Cheng, X (2015) Transcriptome sequencing of mung bean (Vigna radiate L.) genes and the identification of EST-SSR markers. PLOS ONE 10, e0120273.CrossRefGoogle ScholarPubMed
Chueakhunthod, W, Jinagool, W, Meecharoen, K, Khwanman, R, Pattanaram, P, Jantarat, N, Palaphon, P, Ngampongsai, S and Tantasawat, PA (2020) Genetic relationship of mungbean and blackgram genotypes based on agronomic and photosynthetic performance and SRAP markers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 48, 1845–1861.CrossRefGoogle Scholar
Collard, BCY and Mackill, DJ (2008) Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Philosophical Transactions of the Royal Society B 363, 557–572.CrossRefGoogle ScholarPubMed
Divya, B, Robin, S, Rabindran, R, Senthil, S, Raveendran, M and Joel, AJ (2014) Marker-assisted backcross breeding approach to improve blast resistance in Indian rice (Oryza sativa) variety ADT43. Euphytica 200, 61–77.CrossRefGoogle Scholar
Garzón, LN, Ligarreto, GA and Blair, MW (2008) Molecular marker-assisted backcrossing of anthracnose resistance into Andean climbing beans (Phaseolus vulgaris L.). Crop Science 48, 562–570.CrossRefGoogle Scholar
Gopalakrishnan, S, Sharma, RK, Anand Rajkumar, K, Joseph, M, Singh, VP, Bhat, KV, Singh, NK and Mohapatra, T (2008) Integrating marker-assisted background analysis with foreground selection for identification of superior bacterial blight resistant recombinants in Basmati rice. Plant Breeding 127, 131–139.CrossRefGoogle Scholar
Iqbal, SM, Ghafoor, A, Basak, M and Malik, BA (1995) Estimation of losses in yield components of mungbean due to Cercospora leaf spot. Pakistan Journal of Phytopathology 7, 80–81.Google Scholar
Isemura, T, Kaga, A, Tabata, S, Somta, P, Srinives, P, Shimizu, T, Jo, U, Vaughan, DA and Tomooka, N (2012) Construction of a genetic linkage map and genetic analysis of domestication related traits in mungbean (Vigna radiata). PLOS ONE 7, e41304.CrossRefGoogle Scholar
Jamaloddin, Md, Durga Rani, ChV, Swathi, G, Anuradha, Ch, Vanisri, S, Rajan, CPD, Krishnam Raju, S, Bhuvaneshwari, V, Jagadeeswar, R, Laha, GS, Prasad, MS, Satyanarayana, PV, Cheralu, C, Rajani, G, Ramprasad, E, Sravanthi, P, Arun Prem Kumar, N, Aruna Kumari, K, Yamini, KN, Mahesh, D, Sanjeev Rao, D, Sundaram, RM and Sheshu Madhav, M (2020) Marker-assisted gene pyramiding (MAGP) for bacterial blight and blast resistance into mega rice variety “Tellahamsa”. PLOS ONE 15, e0234088.CrossRefGoogle ScholarPubMed
Khajudparn, P, Wongkaew, S and Tantasawat, P (2010) Identification of genes for powdery mildew resistance in mungbean. Journal of Life Sciences 4, 25–29.Google Scholar
Krishna, MSR, Surender, M and Reddy, SS (2017) Marker-assisted breeding for introgression of opaque-2 allele into elite maize inbred line BML-6. Acta Ecologica Sinica 37, 340–345.CrossRefGoogle Scholar
Kumar, V, Rani, A, Anshu, AK and Tayalkar, T (2021) Marker-assisted stacking of null Kunitz trypsin inhibitor and off-flavour generating lipoxygenase-2 in soybean. The Journal of Agricultural Science 159, 272–280.CrossRefGoogle Scholar
Lodhi, MA, Ye, GN, Weeden, NF and Reisch, BI (1994) A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Molecular Biology Reporter 12, 6–13.CrossRefGoogle Scholar
Maranna, S, Verma, K, Talukdar, A, Lal, SK, Kumar, A and Mukherjee, K (2016) Introgression of null allele of Kunitz trypsin inhibitor through marker-assisted backcross breeding in soybean (Glycine max L. Merr.). BMC Genetics 17, 106–114.CrossRefGoogle Scholar
Miah, G, Rafii, MY, Ismail, MR, Puteh, AB, Rahim, HA and Latif, MA (2015) Recurrent parent genome recovery analysis in a marker-assisted backcrossing program of rice (Oryza sativa L.). Comptes Rendus Biologies 338, 83–94.CrossRefGoogle Scholar
Nair, R and Schreinemachers, P (2020) Global status and economic importance of mungbean. In Nair, R, Schafleitner, R and Lee, SH (eds), The Mungbean Genome. Cham: Springer International Publishing, pp. 1–8.CrossRefGoogle Scholar
Nair, RM, Pandey, AK, War, AR, Hanumantharao, B, Shwe, T, Alam, AKMM, Pratap, A, Malik, SR, Karimi, R, Mbeyyagala, EK, Douglas, CA, Rane, J and Schafleitner, R (2019) Biotic and abiotic constraints in mungbean production–progress in genetic improvement. Frontiers in Plant Science 10, 1340–1363.CrossRefGoogle ScholarPubMed
Office of Agricultural Economics (2019) Information of agricultural economics 2019 database. Available at http://www.oae.go.th/assets/portals/1/files/ebook/2563/commodity-2562.pdf (Retrieved 6 June 2021).Google Scholar
Papan, P, Chueakhunthod, W, Poolsawat, O, Arsakit, K, Tharapreuksapong, A and Tantasawat, PA (2021) Validation of molecular markers linked to Cercospora leaf spot disease resistance in mungbean [Vigna Radiata (L.) Wilczek]. Sabrao Journal of Breeding and Genetics 53, 749–757.CrossRefGoogle Scholar
Poolsawat, O, Kativat, C, Arsakit, K and Tantasawat, PA (2017) Identification of quantitative trait loci associated with powdery mildew resistance in mungbean using ISSR and ISSR-RGA markers. Molecular Breeding 37, 150–161.CrossRefGoogle Scholar
Pradhan, SK, Nayak, DK, Mohanty, S, Behera, L, Barik, SR, Pandit, E, Lenka, S and Anandan, A (2015) Pyramiding of three bacterial blight resistance genes for broad-spectrum resistance in deepwater rice variety, Jalmagna. Rice 8, 19–32.CrossRefGoogle ScholarPubMed
Rohlf, FJ (2000) NTSYS Numerical Taxonomy and Multivariate Analysis System Version 2.1 User Guide. New York, NY: Applied Biostatistics, Inc.Google Scholar
Sundaram, RM, Vishnupriya, MR, Biradar, SK, Laha, GS, Reddy, GA, Rani, NS, Sharma, NP and Sonti, RV (2008) Marker-assisted introgression of bacterial blight resistance in Samba Mahsuri, an elite indica rice variety. Euphytica 160, 411–422.CrossRefGoogle Scholar
Tantasawat, PA, Poolsawat, O, Arsakit, K and Papan, P (2020) Identification of ISSR, ISSR-RGA and SSR markers associated with Cercospora leaf spot resistance gene in mungbean. International Journal of Agriculture and Biology 23, 447–453.Google Scholar
Tantasawat, PA, Poolsawat, O, Kativat, C, Arsakit, K, Papan, P, Chueakhunthod, W and Pookhamsak, P (2021) Association of ISSR and ISSR-RGA markers with powdery mildew resistance gene in mungbean. Legume Research 44, 1164–1171.Google Scholar
Tembo, B, Sibiya, J, Tongoona, P and Tembo, L (2017) Validation of microsatellite molecular markers linked with resistance to Bipolaris sorokiniana in wheat (Triticum aestivum L.). The Journal of Agricultural Science 155, 1061–1068.CrossRefGoogle Scholar
Varshney, RK, Mohan, SM, Gaur, PM, Chamarthi, SK, Singh, VK, Srinivasan, S, Swapna, N, Sharma, M, Singh, S, Kaur, L and Pande, S (2014) Marker-assisted backcrossing to introgress resistance to Fusarium wilt race 1 and Ascochyta blight in C 214, an elite cultivar of chickpea. The Plant Genome 7, 1–11.CrossRefGoogle Scholar
Wongpiyasatid, A, Chotechuen, S, Hormchan, P and Srihuttagum, M (1999) Evaluation of yield and resistance to powdery mildew, Cercospora leaf spot and cowpea weevil in mungbean mutant lines. Kasetsart Journal (Natural Science) 33, 204–215.Google Scholar
Xiao, WM, Luo, LX, Wang, H, Guo, T, Liu, YZ, Zhou, JY, Zhu, XY, Yang, QY and Chen, ZQ (2016) Pyramiding of Pi46 and Pita to improve blast resistance and to evaluate the resistance effect of the two R genes. Journal of Integrative Agriculture 15, 2290–2298.CrossRefGoogle Scholar
Yadawad, A, Gadpale, A, Hanchinal, RR, Nadaf, HL, Desai, SA, Biradar, S and Naik, VR (2017) Pyramiding of leaf rust resistance genes in bread wheat variety DWR 162 through marker-assisted backcrossing. Indian Journal of Genetics and Plant Breeding 77, 251–257.CrossRefGoogle Scholar
Yundaeng, C, Somta, P, Chen, J, Yuan, X, Chankaew, S and Chen, X (2020) Fine mapping of QTL conferring Cercospora leaf spot disease resistance in mungbean revealed TAF5 as candidate gene for the resistance. Theoretical and Applied Genetics 134, 701–714.CrossRefGoogle ScholarPubMed