Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-26T02:39:06.438Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification of phyto-oestrogens in berseem clover (Trifolium alexandrinum)

Published online by Cambridge University Press:  27 March 2009

M. Shemesh ,
N. Ayalon  and
H. R. Lindner
M. Shemesh
Affiliation:
Departments of Biochemistry and Reproduction, Kimron Veterinary Institute, Beit Dagan
N. Ayalon
Affiliation:
Departments of Biochemistry and Reproduction, Kimron Veterinary Institute, Beit Dagan
H. R. Lindner
Affiliation:
Department of Hormone Research, The Weizmann Institute of Science, Rehovot, Israel
Get access Rights & Permissions [Opens in a new window]

Summary

The oestrogenic isoflavones genistein, biochanin-A and formononetin, as well as the coumestan derivative coumestrol, were identified by thin-layer, paper and gas chromatography in berseem clover (Trifolium alexandrinum) grown in Israel. Under the conditions studied, the concentration of these oestrogens in the berseem clover (total < 0·01% in the dry matter) was considerably lower than those found in clover species known to be associated with infertility in ruminants.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 87 , Issue 3 , December 1976 , pp. 467 - 469
Copyright
Copyright © Cambridge University Press 1976

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Beck, A. B. (1964). The oestrogenio isoflavones of subterranean clover. Australian Journal of Agricultural Research 15, 223–30.Google Scholar
Bennetts, H. W., Underwood, E. J. & Shier, F. L. (1946). A specific breeding problem of sheep on subterranean clover pastures in Western Australia. Australian Veterinary Journal 22, 2–12.CrossRefGoogle ScholarPubMed
Bickoff, E. M. (1968). Estrogenic constituents of forage plants. Commonwealth Agricultural Review Serial No. 1, 1–53.Google Scholar
Guggolz, J., Livingston, A. L. & Bickoff, E. M. (1961). Detection of daidzein, formononetin, genistein and biochanin A in forages. Journal of Agricultural and Food Chemistry 9, 330–2.Google Scholar
Kallela, K. K. (1964). On the oestrogenio effects of red clover fodder on sheep. Nordisk Veterinaermedicin 16, 731–43.Google Scholar
Kapoor, P. D. & Pal, A. K. (1964). Estrogenic activity of berseem (Trifolium alexandrinum). Indian Veterinary Journal 41, 598–604.Google Scholar
Lindner, H. R. (1967). Study of the fate of phytooestrogens in the sheep by determination of isoflavones and coumestrol in the plasma and adipose tissue. Australian Journal of Agricultural Research 18, 305–33.CrossRefGoogle Scholar
Linstedt, G. (1950). Constituents of pine heartwood. Acta Chemica Scandinavica 4, 448–55.Google Scholar
Loper, G. M. & Hanson, C. H. (1967). Influence of controlled coumostrol environmental factors and two foliar pathogens in alfalfa. Crop Science 7, 189–92.Google Scholar
Nilsson, A. (1961). On the in vitro metabolism of the plant oestrogen Biochanin A in rumen fluid. Ark. Kemi 17, 305.Google Scholar
Pope, G. S., McNaughton, M. J. & Jones, H. E. H. (1959). Oestrogen in British pasture plants. Journal of Dairy Research 26, 196–202.CrossRefGoogle Scholar
Saba, N., Drane, H. M., Hebert, C. N., Newton, J. E. & Betts, J. E. (1972). Effect of disease on the oestrogenic activity and coumestrol content of white clover and lucerne. Journal of Agricultural Science, Cambridge 78, 471–5.CrossRefGoogle Scholar
Sharaf, A. (1968). Oestrogenicity of food plants and their possible effects on fertility in animals. Proceedings VI International Congress on Animal Reproduction and I.A., Paris 1, 805–7.Google Scholar
Shemesh, M., Lindner, H. R. & Ayalon, N. (1969). Coumestrol and 4'-O-methyl-coumestrol in alfalfa grown in northern Israel: possible effect of a foliar pathogen (Pseudopeziza medicaginis Lib.). Refuah Veterinarit 26, 1–7.Google Scholar
Shutt, D. A. & Braden, A. W. H. (1967). The significance of equol in relation to oestrogenic responses in sheep ingesting clover with high formononetin content. Australian Journal of Agricultural Research 19, 545–53.Google Scholar
Wong, E. & Flux, D. S. (1962). The oestrogenio activity of red clover isoflavones and some of their degradation products. Journal of Endocrinology 24, 341–8.CrossRefGoogle ScholarPubMed