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The identification and genetic diversity of endophytic bacteria isolated from selected crops

Published online by Cambridge University Press:  14 August 2018

M. Woźniak ,
A. Gałązka ,
J. Grządziel  and
M. Głodowska
M. Woźniak*
Affiliation:
Department of Agricultural Microbiology, Institute of Soil Science and Plant Cultivation – State Research Institute, Czartoryskich Street 8, 24-100 Pulawy, Poland
A. Gałązka
Affiliation:
Department of Agricultural Microbiology, Institute of Soil Science and Plant Cultivation – State Research Institute, Czartoryskich Street 8, 24-100 Pulawy, Poland
J. Grządziel
Affiliation:
Department of Agricultural Microbiology, Institute of Soil Science and Plant Cultivation – State Research Institute, Czartoryskich Street 8, 24-100 Pulawy, Poland
M. Głodowska
Affiliation:
Department of Agricultural Microbiology, Institute of Soil Science and Plant Cultivation – State Research Institute, Czartoryskich Street 8, 24-100 Pulawy, Poland
*
Author for correspondence: M. Woźniak, E-mail: [email protected]
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Abstract

A collection of 45 isolates was created based on bacteria isolated from maize, broad bean, wheat, rye and wild plants such as horsetail and burdock. The aim of the current study was to isolate the bacteria, and then identify and assess the degree of genomic diversity. The molecular identification of microsymbionts isolated from the endosphere (root and stem) of plants grown in agricultural soils was performed using 16S rRNA gene sequencing. To evaluate the genomic diversity between strains that occurred in multiple host plants, 18 bacterial isolates representing four species were subjected to denaturing gradient gel electrophoresis. The 16S rDNA analysis assigned all bacterial isolates to ten genera, from which Rhizobium was represented by 19 isolates, Delftia by 11, Agrobacterium by five, Stenotrophomonas by three, Brevundimonas by two and Novosphingobium, Variovorax, Collimonas, Achromobacter and Comamonas by only one isolate. Furthermore, the genomic diversity of the 11 isolates of Delftia sp. was assessed using the BOX – polymerase chain reaction (BOX-PCR) and enterobacterial repetitive intergenic consensus – PCR (ERIC-PCR) methods. Typing patterns and analysis using BOX-PCR and ERIC-PCR data demonstrated similarities among the tested isolates. In general, the results obtained with BOX-PCR and ERIC-PCR were in good agreement. However, a greater degree of differentiation patterns of the genomic DNA was obtained in the ERIC-PCR method.

Keywords

16S rRNA genebiodiversityendophytesPCR
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 156 , Issue 4 , May 2018 , pp. 547 - 556
Copyright
Copyright © Cambridge University Press 2018 

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