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Hydrodynamics and the germination of oil-seed rape pollen

Published online by Cambridge University Press:  27 March 2009

Sarah A. Corbet  and
Janette R. Plumridge
Sarah A. Corbet
Affiliation:
Department of Applied Biology, Pembroke Street, Cambridge, CB2 3DX
Janette R. Plumridge
Affiliation:
Department of Applied Biology, Pembroke Street, Cambridge, CB2 3DX
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Summary

Pollen grains of oil-seed rape, Brassica napus, were germinated in vitro in conditions in which water activity could be closely controlled. Germination success depended on the humidity experienced by the grains before contact with the germination medium, as well as on the osmolarity of that medium. On contact with the germination medium the grains swelled and then shrank. Each suffered one of three fates: it burst, or it germinated without bursting, or it did neither, remaining unchanged. Grains that were to remain unchanged swelled least. Those that were to burst swelled most. Those that were to germinate were intermediate.

Some implications for in vitro germination studies are discussed.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 104 , Issue 2 , April 1985 , pp. 445 - 451
Copyright
Copyright © Cambridge University Press 1985

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