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Glucose and fatty acid metabolism in cows producing milk of low fat content

Published online by Cambridge University Press:  27 March 2009

E. F. Annison ,
R. Bickerstaffe  and
J. L. Linzell
E. F. Annison
Affiliation:
Unilever Research Laboratory, Colworth House, Sharnbrook, Bedford
R. Bickerstaffe
Affiliation:
Unilever Research Laboratory, Colworth House, Sharnbrook, Bedford
J. L. Linzell
Affiliation:
A.R.C. Institute of Animal Physiology, Babraham, Cambridge
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Summary

The effects of changing to a high starch: low roughage diet have been studied in two Friesian and two Jersey cows, surgically prepared for the simultaneous study of udder metabolism (arteriovenous difference x udder blood flow) and whole body turnover of milk precursors (isotope dilution).

In the Friesian cows milk fat concentration was lower on the high starch diet but in the Jerseys fell only slightly in one animal. In both Friesians and in the one Jersey these changes were accompanied by an increase in total rumen VFA concentration. Rumen acetate concentration did not change but propionate doubled. Thus this confirms that the usually reported fall in ‘acetate:propionate ratio’ is due to a rise in propionate production rather than due to a fall in acetate production.

There were significant falls in the blood concentrations of acetate and β-hydroxy-butyrate. The rate of extraction by the udder of acetate and β-hydroxybutyrate did not change but triglyceride extraction fell. Therefore since udder blood flow did not alter the uptake of all three fat precursors fell.

The entry rate of glucose into the circulation and its contribution to total body CO2 increased. The entry rate and contribution to CO2 of acetate decreased but this was probably mainly due to a fall in endogenous acetate production by the body tissues. Plasma FFA concentration showed little change but the entry rate of palmitate fell on the high starch diet. There was also an increased proportion of unsaturated and trans fatty acids in the plasma and milk triglycerides.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 82 , Issue 1 , February 1974 , pp. 87 - 95
Copyright
Copyright © Cambridge University Press 1974

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References

REFERENCES

Annison, E. F. (1954). Studies on the volatile fatty acids of sheep blood with special reference to formic acid. Biochemical Journal 58, 670–80.CrossRefGoogle ScholarPubMed
Annison, E. F., Linzell, J. L., Fazakerley, S. & Nichols, B. W. (1967). The oxidation and utilization of palmitate, stearate, oleate and acetate by the mammary gland of the fed goat in relation to their overall metabolism and the role of plasma phospholipids and neutral lipids in milk-fat synthesis. Biochemical Journal 102, 637–47.CrossRefGoogle ScholarPubMed
Annison, E. F., Linzell, J. L. & West, C. E. (1968). Mammary and whole animal metabolism of glucose and fatty acids in fasting lactating goats. Journal of Physiology, London 197, 445–59.CrossRefGoogle ScholarPubMed
Annison, E. F. & White, R. R. (1961). Glucose utilization in sheep. Biochemical Journal 80, 162–9.CrossRefGoogle ScholarPubMed
Annison, E. F. & White, R. R. (1962). Formate metabolism in sheep. Biochemical Journal 84, 552–7.CrossRefGoogle ScholarPubMed
Bergman, E. N. & Wolff, J. E. (1971). Metabolism of volatile fatty acids by liver and portal-drained viscera in sheep. American Journal of Physiology 221, 586–92.CrossRefGoogle ScholarPubMed
Bickerstaffe, R. & Annison, E. F. (1970). Lipid metabolism in the perfused chicken liver; uptake and metabolism of oleic acid, elaidic acid, cis-vaccenic acid, trans-vaccenic acid and stearic acid. Biochemical Journal 118, 433–42.CrossRefGoogle ScholarPubMed
Bickerstaffe, R. & Annison, E. F. (1971). Triglyceride synthesis in goat and sow mammary tissue. International Journal of Biochemistry 2, 153–62.CrossRefGoogle Scholar
Bickerstaffe, R., Annison, E. F. & Linzell, J. L. (1974). The metabolism of glucose, acetate, lipids and amino acids in the lactating dairy cow. Journal of Agricultural Science, Cambridge 82, 71–85.CrossRefGoogle Scholar
Bickerstaffe, R., West, C. E. & Annison, E. F. (1970). Lipid metabolism in the perfused chicken liver: lipogenesis from glucose, acetate and palmitate. Biochemical Journal 118, 427–31.CrossRefGoogle ScholarPubMed
Davis, C. L. (1967). Acetate production in the rumen of cows fed either control or low-fibre, high-grain diets. Journal of Dairy Science 50, 1621–5.CrossRefGoogle ScholarPubMed
Davis, C. L. & Brown, R. E. (1970). Low-fat milk syndrome. In Physiology of Digestion and Metabolism in the Ruminant (ed. Phillipson, A. T.), pp. 545–65. Newcastle upon Tyne: Oriel Press.Google Scholar
Hamosh, M., Clary, T. R., Chernick, S. S. & Scow, R. O. (1970). Lipoprotein lipase activity of adipose and mammary tissue and plasma triglyceride in pregnant and lactating rats. Biochimica et biophysica Acta 210, 473–82.CrossRefGoogle ScholarPubMed
Huggett, A. St G. & Nixon, D. A. (1957). Enzymic determination of blood glucose. Biochemical Journal 66, 12P.Google Scholar
Jones, G. B. (1965). Determination of specific activity of labelled blood glucose by liquid scintillation counting using glucose pentaacetate. Analytical Biochemistry 12, 249–58.CrossRefGoogle Scholar
Leng, R. A. (1970). Formation and production of volatile fatty acids in the rumen. In Physiology of Digestion and Metabolism in the Ruminant (ed. Phillipson, A. T.), pp. 406–21. Newcastle upon Tyne: Oriel Press.Google Scholar
Leng, R. A. & West, C. E. (1969). Contribution of acetate, butyrate, palmitate, stearate and oleate to ketone body synthesis in sheep. Research in Veterinary Science 10, 57–63.CrossRefGoogle ScholarPubMed
McClymont, G. L. & Vallance, S. (1962). Depression of blood glycerides and milk fat synthesis by glucose infusion. Proceedings of the Nutrition Society 21, xli.Google Scholar
Storry, J. E. & Sutton, J. D. (1969). The effect of change from low-roughage to high-roughage diets on rumen fermentation, blood composition and milk fat secretion in the cow. British Journal of Nutrition 23, 511–21.CrossRefGoogle ScholarPubMed
Van Soest, P. J. (1963). Ruminant fat metabolism with particular reference to factors affecting low milk fat and feed efficiency. A review. Journal of Dairy Science 46, 204–16.CrossRefGoogle Scholar
West, C. E., Bickerstaffe, R., Annison, E. F. & Linzell, J. L. (1972). Studies on the mode of uptake of blood triglycerides by the mammary gland of the lactating goat. Biochemical Journal 126, 477–90.CrossRefGoogle ScholarPubMed
West, C. E. & Rowbotham, T. R. (1967). The use of a computer in the determination by gas–liquid chromatography of the concentration and identification of individual fatty acids present as free fatty acids, triglycerides and cholesterol esters. Journal of Chromatography 30, 62–76.CrossRefGoogle Scholar