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Foxtail millet WRKY genes and drought stress

Part of: AGS Millet Collection

Published online by Cambridge University Press:  02 November 2016

L. ZHANG ,
H. SHU ,
A. Y. ZHANG ,
B. L. LIU ,
G. F. XING ,
J. A. XUE ,
L. X. YUAN ,
C. Y. GAO  and
R. Z. LI
L. ZHANG
Affiliation:
Institute of Molecular Agriculture & Bioenergy, Shanxi Agricultural University, Taigu 030801, China
H. SHU
Affiliation:
Program of Molecular Medicine, University of Massachusetts Medical School, Massachusetts 01605, USA
A. Y. ZHANG
Affiliation:
Institute of Millet, Shanxi Academy of Agricultural Sciences, Changzhi 046011, China
B. L. LIU
Affiliation:
Institute of Molecular Agriculture & Bioenergy, Shanxi Agricultural University, Taigu 030801, China
G. F. XING
Affiliation:
Institute of Molecular Agriculture & Bioenergy, Shanxi Agricultural University, Taigu 030801, China
J. A. XUE
Affiliation:
Institute of Molecular Agriculture & Bioenergy, Shanxi Agricultural University, Taigu 030801, China
L. X. YUAN
Affiliation:
Institute of Molecular Agriculture & Bioenergy, Shanxi Agricultural University, Taigu 030801, China
C. Y. GAO
Affiliation:
Institute of Molecular Agriculture & Bioenergy, Shanxi Agricultural University, Taigu 030801, China
R. Z. LI*
Affiliation:
Institute of Molecular Agriculture & Bioenergy, Shanxi Agricultural University, Taigu 030801, China
*
*To whom all correspondence should be addressed. Email: [email protected]
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Summary

Foxtail millet (Setaria italica (L.) P. Beauv.) is a naturally stress-tolerant plant, a major reserve crop and a model for panicoid grasses. The recent completion of the S. italica genome facilitates identification and characterization of WRKY transcription factor family proteins that are important regulators of major plant processes, including growth, development and stress response. The present study identified 103 WRKY transcription factor-encoding genes in the S. italica genome. The genes were named SiWRKY1–SiWRKY103 according to their order on the chromosomes. A comprehensive expression analysis of SiWRKY genes among four different tissues was performed using publicly available RNA sequencing data. Eighty-four SiWRKY genes were more highly expressed in root tissue than in other tissues and nine genes were only expressed in roots. Additionally, real-time quantitative polymerase chain reaction was performed to comprehensively analyse the expression of all SiWRKY genes in response to dehydration. Results indicated that most SiWRKY genes (over 0.8) were up-regulated by drought stress. In conclusion, genome-wide identification and expression profiling of SiWRKY genes provided a set of candidates for cloning and functional analyses in plants’ response to drought stress.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 155 , Issue 5 , July 2017 , pp. 777 - 790
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Copyright
Copyright © Cambridge University Press 2016 

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Footnotes

†

The first two authors contributed equally to this paper.

References

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