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Fermentation profile, microbial populations, taxonomic diversity and aerobic stability of total mixed ration silages based on Cactus and Gliricidia

Published online by Cambridge University Press:  14 October 2020

F. N. S. Santos Open the ORCID record for F. N. S. Santos [Opens in a new window] ,
E. M. Santos ,
J. S. Oliveira ,
G. R. Medeiros ,
A. M. Zanine Open the ORCID record for A. M. Zanine [Opens in a new window] ,
G. G. L. Araújo ,
A. F. Perazzo Open the ORCID record for A. F. Perazzo [Opens in a new window] ,
M. L. P. Lemos ,
D. M. Pereira  and
G. F. L. Cruz Open the ORCID record for G. F. L. Cruz [Opens in a new window]
...Show all authors
F. N. S. Santos*
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia/PB 58397-000, Brazil
E. M. Santos
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia/PB 58397-000, Brazil
J. S. Oliveira
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia/PB 58397-000, Brazil
G. R. Medeiros
Affiliation:
National Semi-Arid Institute, Campina Grande/PB 58.434-700, Brazil
A. M. Zanine
Affiliation:
Department of Animal Science, Federal University of Maranhão, Chapadinha/MA 65500-000, Brazil
G. G. L. Araújo
Affiliation:
Brazilian Agricultural Research Corporation, Petrolina/PE 56302-970, Brazil
A. F. Perazzo
Affiliation:
Department of Animal Science, Federal University of Maranhão, Chapadinha/MA 65500-000, Brazil
M. L. P. Lemos
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia/PB 58397-000, Brazil
D. M. Pereira
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia/PB 58397-000, Brazil
G. F. L. Cruz
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia/PB 58397-000, Brazil
R. S. Paulino
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia/PB 58397-000, Brazil
C. J. B. Oliveira
Affiliation:
Department of Animal Science, Federal University of Paraíba, Areia/PB 58397-000, Brazil
*
Author for correspondence: F. N. S Santos, E-mail: [email protected]
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Abstract

The aim of this study was to investigate the effects of Cactus (Opuntia spp) levels in total mixed ration silages based on Cactus and Gliricidia (Gliricidia sepium (Jacq.) Steud) on the fermentation profile, microbial populations, aerobic stability and taxonomic diversity. The completely randomized design was used in a 4 × 4 factorial design with four replications, being four rations with different levels of Cactus (15, 30, 45, 60% based on the dry matter) and four opening periods (0, 15, 30 and 60 days of fermentation). An interaction effect (P < 0.050) was observed among the diets and opening times for mould and yeast populations. An interaction effect for the levels of acetic acid was observed, where the diets 15, 30, 45 and 60% showed higher values at 60 days (0.44, 0.41, 0.35 and 0.40 g/kg DM, respectively). A significant difference was observed for the richness and diversity index (Chao1 and Shannon). The most abundant bacterial phyla were Proteobacteria and Firmicutes and the genera Lactobacillus and Weissella. Cactus can be added in total mixed ration silages up to the level of 60% in a way that it positively affects the qualitative indicators of the silages, modulating the taxonomic communities and allowing the predominance of important groups for preservation of the ensiled mass.

Keywords

aerobic stabilitylactic acidlactic bacteriasequencing
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 158 , Issue 5 , July 2020 , pp. 396 - 405
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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Footnotes

*

Part of the doctoral thesis of the first author

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