Published online by Cambridge University Press: 27 March 2009
A quantitative evaluation of the pigment in Murrah buffaloes of both sexes ranging in age from 1½ to 7 years revealed that dorsal areas had most pigment, while the lateral body areas and the extremities were intermediate and the ventral and axillary regions revealed the least pigment. Maximum concentration of melanin granules occurred in stratum cylindricum, which gradually declined towards stratum corneum which was fully non-pigmented in axilla, groin, inter-digital and inter-dewclaw areas.
Pigment granules blackened in silver preparations and were seen concentrated as protective ‘nuclear caps’ in the supranuclear zone of the cells of stratum germinativum. The infranuclear light-staining cytoplasm contained PAS reactive glycogen granules.