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Effects of dietary deficiencies of energy, protein and calcium on the pregnant ewe: V. Chemical analyses and histological examination of some individual bones

Published online by Cambridge University Press:  27 March 2009

A. R. Sykes ,
D. I. Nisbet  and
A. C. Field
A. R. Sykes
Affiliation:
Moredun Research Institute, Edinburgh EH 17 7JH
D. I. Nisbet
Affiliation:
Moredun Research Institute, Edinburgh EH 17 7JH
A. C. Field
Affiliation:
Moredun Research Institute, Edinburgh EH 17 7JH
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Summary

The effects of low protein and low Ca intakes during pregnancy on the chemical composition and histological appearance of selected bones of ewes and lambs is reported. Twenty-eight 6½-year-old Blackface ewes were used in a 2 x 2 experiment in which semi-purified diets containing 11·8 and 6·0% crude protein and 1·2 and 0·11% Ca in the dry matter were offered in amounts designed to induce a level of undernutrition typical of that experienced by hill sheep in winter. In addition, six sheep were killed in early pregnancy as controls, and a further six were offered, during pregnancy, a conventional diet containing 16% crude protein and 1·9% Ca in the dry matter at levels which prevented energy undernutrition. Ewes and lambs were killed at parturition and the tibias, 3rd ribs and 3rd and 4th lumbar vertebrae removed for chemical and histological examination.

The degree of mineralization of the organic matrix (ash:organic-matter ratio) of the bones at the beginning of pregnancy was poor and it increased in all groups during pregnancy largely irrespective of protein or Ca intake, but especially in the wellnourished sheep. The density of ash in the whole bone (ash:volume ratio) increased in the well-nourished and high-protein groups but did not change in the low-protein groups. These differences were attributable to the effect of protein nutrition on the density of the bone organic matrix (organic matter:volume ratio). Matrix density of the bones of the well-nourished groups increased while that of the low-protein groups decreased.

The histological data essentially confirmed this picture and showed that trabecular or cancellous bone from the tibial head and vertebrae of the low-protein and to a lesser extent the high-protein sheep was porotic, but microscopic assessment conflicted with the chemical findings in that histologically Ca intake appeared to affect bone quality in the vertebrae.

There was considerable variation in the histology of individual lamb bones which was independent of maternal nutritional experiences, but the overall size of the bones was determined by maternal protein nutrition.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 81 , Issue 3 , December 1973 , pp. 433 - 440
Copyright
Copyright © Cambridge University Press 1973

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References

REFERENCES

Agricultural Research Council (1965). The Nutrient Requirements of Farm Livestock, no. 2. Ruminants. London: Agricultural Research Council.Google Scholar
Bell, G. H., Cuthbertson, D. P. & Orr, J. (1941). Strength and size of bone in relation to calcium intake. J. Physiol. 100, 299–317.Google Scholar
Benzie, D., Boyne, A. W., Dalgarno, A. C., Duckworth, J., Hill, R. & Walkeb, D. M. (1950). Studies of the skeleton of the sheep. II. The relationship between calcium intake and resorption and repair of the skeleton in pregnancy and lactation. J. agric. Sci., Camb. 48, 175–86.Google Scholar
Benzie, D., Boyne, A. W., Daloarno, A. C., Duckworth, J. & Hill, R. (1959). Studies of the skeleton of the sheep. III. The relationship between phosphorus intake and resorption and repair of the skeleton in pregnancy and lactation. J. agric. Sci., Camb. 52, 1–12.Google Scholar
Chick, H. & Roscoe, M. H. (1926). The anti-rachitic value of fresh spinach. Biochem. J. 20, 137—51.Google Scholar
Chick, H., Korenchevsky, V. & Roscoe, M. H. (1926). Difference in chemical composition of skeletons of young rats fed on diets deprived of fat soluble vitamins and on low phosphorus rachitic diets compared with those of normally nourished animals of same age. Biochem. J. 20, 622–31.Google Scholar
Clayden, E. C. (1952). A discussion on the preparation of bone sections by the paraffin wax method with special reference to the control of decalcification. J. Med. Lab. Tech. 10, 103–23.Google Scholar
El-Maraghi, N. R. H., Platt, B. S. & Stewart, R. J. C. (1965). The effect of the interaction of dietary protein and calcium on the growth and maintenance of the bones of young, adult and aged rats. Br. J. Nutr. 19, 491–509.Google Scholar
El-Maraghi, N. R. H., Platt, B. S. & Stewakt, R. J. C. (1966). The effect of reproduction on the interaction of dietary protein and calcium. Br. J. Nutr. 20, 733–45.CrossRefGoogle Scholar
Field, A. C., Suttle, N. F. & Gunn, R. G. (1968). Seasonal changes in the composition and mineral content of the body of hill sheep. J. agric. Sci., Camb. 71, 303–10.Google Scholar
Fontaine, R., Mandel, P. & Gries, A. (1950). Nouvelles données sur l'évolution biochimique de l'os au cours de jeune prolonge chez le rat. C. r. Séanc. Soc. Biol. 114, 1397–99.Google Scholar
Frandsen, A. M., Nelson, N. M., Sulon, E., Becks, H. & Evans, H. M. (1954). The effects of various levels of dietary protein on skeletal growth and endochondral ossification in young rats. Anat. Rec. 119, 247–73.CrossRefGoogle Scholar
Harrison, M. & Fraser, R. (1960). Bone structure and metabolism in calcium-deficient rats. J. Endocrin. 21, 197–205.Google Scholar
McCance, R. A. & Widdowson, E. M. (1962). Nutrition and growth. Proc. R. Soc. B 156, 326–37.Google Scholar
Russel, A. J. F., Doney, J. M. & Reid, R. L. (1967). The use of biochemical parameters in controlling nutritional state in pregnant ewes, and the effect of undernourishment during pregnancy on lamb birth weight. J. agric. Sci., Camb. 68, 351—58.Google Scholar
Russel, A. J. F., Gunn, R. G. & Doney, J. M. (1968). Components of weight loss in pregnant hill ewes in winter. Anim. Prod. 10, 43–51.Google Scholar
Snedicor, G. W. (1956). Statistical Methods. Iowa, U.S.A.: Iowa State University Press.Google Scholar
Stewart, R. J. C. (1965). Bone pathology in experimental malnutrition. Wld Rev. Nutr. Diet. 5, 275–337.Google Scholar
Sykes, A. R. & Field, A. C. (1972 a). Effects of dietary deficiencies of energy, protein and calcium on the pregnant ewe. I. Body composition and mineral content of the ewes. J. agric. Sci., Camb. 78, 109–17.Google Scholar
Sykes, A. R. & Field, A. C. (1972 b). Effects of dietary deficiencies of energy, protein and calcium on the pregnant ewe. II. Body composition and mineral content of the lamb. J. agric. Sci., Camb. 78, 119–25.Google Scholar