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The effect on conception rates of semen diluents containing citrate or phosphate buffer, with all combinations of sulphanilamide, streptomycin and penicillin

Published online by Cambridge University Press:  27 March 2009

R. C. Campbell  and
J. Edwards
R. C. Campbell
Affiliation:
A.R.C. Unit of Animal Reproduction, University of Cambridge
J. Edwards
Affiliation:
M.M.B., Thames Ditton, Surrey
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Extract

1. A 24 factorial experiment involving 68,713 first inseminations was carried out at four artificial insemination centres, to compare semen diluents containing citrate or phosphate buffers and all combinations of sulphanilamide, streptomycin and penicillin.

2. The analysis of the results was carried out on punched card machines and on an automatic calculator (EDSAC), as well as on bench machines.

3. The main results obtained were:

(i) Unsupplemented phosphate buffer gave a conception rate 5·5% above that obtained with unsupplemented citrate buffer.

(ii) Citrate buffer with sulphanilamide, streptomycin and penicillin gave a conception rate 6·8% above that obtained with unsupplemented citrate buffer; and

(iii) Phosphate buffer with penicillin gave a conception rate 9·3% above that obtained with unsupplemented citrate buffer. This effect was not, however, significantly greater than that referred to in (ii).

4. Phosphate buffer depressed conception rate when used with sulphanilamide or streptomycin.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 46 , Issue 1 , June 1955 , pp. 44 - 55
Copyright
Copyright © Cambridge University Press 1955

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